Increased blastomere number is associated with higher live birth rate in day 3 embryo transfer

Tian, Lifeng; Xia, Leizhen; Liu, Hongbo; Kou, Yan; Huang, Zhihui; Wu, Xingwu; Fan, Lu; Huang, Jialyu; Wu, Qiongfang

doi:10.1186/s12884-022-04521-5

Cited by 10 publications

(4 citation statements)

References 34 publications

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“…Their results showed that for embryos with normal cleavage patterns, developmental potential, implantation, and LBRs increased with cell number. Moreover, Tian et al [15] found that the LBRs were signi cantly higher in patients transplanted with 10-cell embryos (OR 1.62, 95% CI 1.03-2.53; P = 0.035) and ≥ 11-cell embryos (OR 2.14, 95% CI 1.47-3.11; P < 0.001) than in those transplanted with 8-cell embryos. These results are consistent with those of our study.…”

Section: Discussionmentioning

confidence: 98%

Impact of Day 3 Cell Number on the Live Birth Rate of Vitrified-Warmed Single Blastocyst Transfer

Qiu

et al. 2023

Preprint

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Background: Previous studies have reported inconsistent results regarding blastocyst selection with high cell number of day 3 (D3) and the eventual pregnancy outcomes. Thus, this study investigated the relationship between the D3 cell number and clinical outcomes of single blastocyst transfer (SBT) in vitrified-warmed transfer cycles. Methods: A total of 1144 vitrified-warmed cycles of SBT were retrospectively analyzed. Based on the D3 cell number, the cycles were divided into four groups: group A (3–7 cells, n = 130); group B (8–9 cells, n = 621); group C (10–12 cells, n = 328); and group D (13–16 cells, n = 65). The differences in live birth rate (LBR), clinicalpregnancy rate, and miscarriage rate were examined among the four groups. Results: The LBR and clinical pregnancy rate increased with the D3 cell number (P < 0.01). No significant difference was found in the miscarriage rate among the groups (P= 0.055). After adjusting for confounding factors, the LBR was significantly higher in groups C (odds ratio [OR] = 1.506, 95% confidence interval [CI]: 1.148–1.976, P < 0.01) and D (OR = 2.081, 95% CI: 1.218–3.556, P< 0.01) than in group B. Conclusions: A high D3 cell number (>9 cells) was associated with a high LBR in the vitrified-warmed SBT cycle. The cell number of D3 embryos can be an important reference indicator for blastocyst selection. Among blastocysts with the same morphological score, those with >9 cells on D3 can be preferentially selected for transplantation.

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Section: Discussionmentioning

confidence: 98%

Impact of Day 3 Cell Number on the Live Birth Rate of Vitrified-Warmed Single Blastocyst Transfer

Qiu

et al. 2023

Preprint

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“…A low blastomere number in D3 embryos commonly indicates poor prognosis. Studies have con rmed that the transfer of slow-developing embryos results in low implantation rates and LBR [21,22]. In this regard, several possible reasons have been proposed.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, some questions regarding the current consensus on embryo assessment have been raised. Some studies have hypothesized that fast-cleaving embryos have a similar or even higher developmental competence than 8cell embryos [13,21,23,24]. Luna et al [24]demonstrated that fast-cleaving embryos (> 10 cells) not only reach the blastocyst stage at a rate similar to that of intermediate-cleaving embryos (8 cells) but also have a signi cantly greater likelihood of reaching better blastocysts.…”

Section: Discussionmentioning

confidence: 99%

Increased blastomere number in D3 embryos is associated with higher live birth rates in vitrified– thawed single blastocyst transfer cycles

Zheng,

Lin,

et al. 2024

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Background The number of D3 embryo blastomeres affects pregnancy outcomes in patients undergoing cleavage-stage embryo transfer. However, the association between blastomere number in D3 embryos and pregnancy outcomes after vitrified–thawed single blastocyst transfer remains unknown.Methods This retrospective cohort follow-up study included 2,274 cases of vitrified–thawed single blastocyst transfer,all patients were divided into six groups according to blastomere number of D3 embryos: ≤5, 6, 7, 8, 9, and ≥ 10 cells. The primary outcome was the live birth rate (LBR). The secondary outcomes were the clinical pregnancy rate, miscarriage rate, and neonatal outcomes. Statistical analyses were performed using a multivariate logistic regression model to explore the association between blastomere number in D3 embryos and LBR.Results The LBR significantly increased with the number of blastomeres in D3 embryos (28.4%, 36.4%, 42.5%, 46.1%, 45.2%, and 58.1%; p < 0.001). Furthermore, the results of the high- and low-quality blastocyst subgroup analyses showed significant differences in the LBR among the groups (p < 0.01). As the number of blastomeres in D3 embryos increased, the miscarriage rate significantly decreased (23.3%, 18.6%, 14.0%, 15.9%, 13.6%, and 8.9%; p < 0.05). However, the number of blastomeres did not affect perinatal outcomes. Multivariate logistic regression analysis after adjusting for confounding factors revealed significantly decreased LBR in the ≤ 5-cell group (adjusted odds ratio [aOR]: 0.627, 95% confidence interval [CI]: 0.442–0.891; p < 0.01) and significantly increased LBR in the ≥ 10-cell group (aOR: 1.612, 95% CI: 1.230–2.112; p < 0.01) compared with that in the 8-cell group.Conclusions The number of blastomeres in D3 embryos may be an important factor in selecting blastocysts during vitrified–thawed single blastocyst transfer cycles. The transfer of a single blastocyst arising from ≥ 10-blastomere D3 embryo may reduce the miscarriage rate and improve LBR.

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“…Pons MC believed that D3 embryos with more than 11 cell blastomeres had the equivalent developmental potential to those with eight-cell blastomeres (Pons et al, 2019). Tian L used the 8-cell embryo group as a model, and found that patients who received 10-cell and 11-cell embryo transplants had a much higher chance of having a live delivery (Tian et al, 2022). However, some studies have suggested that the pregnancy outcome of fasterdeveloping embryos is lower than that of normal-developing embryos(Martinez-Granados et al, 2018, Racowsky et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Associations between the average blastomere number of all embryos on day 3 and the live birth rate in the transfer cycle: a single-centre, retrospective study

Tian

et al. 2023

Preprint

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Research question: What are the influencing factors of the average blastomere number of all embryos in D3 patients and the association with a live birth rate in the transfer cycle? Methos: A total of 7420 fresh embryo transferred cycles of patients who underwent IVF-ET in the Reproductive Medicine Center, Renmin Hospital, Hubei University of Medicine from January 2016 to December 2021 were selected retrospective observed. Results: According to the average blastomeres number in all embryos on day 3 following oocyte retrieval, the patients were split into five groups: 5.00 group (747 cases), 5.01-6.00 group (1763 cases), 6.01-7.00 group (3052 patients), 7.01-8.00 group (1646 cases), and >8 groups (212 patients). The average number of blastomere on D3 was negatively associated (P <0.05) with infertility, duration infertility, male factors infertility, E2 value on hCG day, and ICSI insemination method. The live birth rate(LBR) of the groups 5.00 and 5.01-6.00 was considerably lower than that of the other groups, according to multivariate regression analysis（P＜0.001). Further additional curve fitting was utilized to demonstrate that the LBR increased significantly as the number of blastomeres grew, increasing a value of 20% for every different blastomere number when the average number of blastomeres on D3＜5.7 (P<0.001). On the other hand, when the number≥5.7 (P=0.258), the live birth rate no longer increases. Conclusion: An average blastomere number between 7.01 and 8.00 is associated with higher LBR in D3 fresh embryo transfer cycle, when the average blastomeres in D3 embryos are less than 5.7 to reduce the live birth rate of the transfer cycle.

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Increased blastomere number is associated with higher live birth rate in day 3 embryo transfer

Cited by 10 publications

References 34 publications

Impact of Day 3 Cell Number on the Live Birth Rate of Vitrified-Warmed Single Blastocyst Transfer

Impact of Day 3 Cell Number on the Live Birth Rate of Vitrified-Warmed Single Blastocyst Transfer

Increased blastomere number in D3 embryos is associated with higher live birth rates in vitrified– thawed single blastocyst transfer cycles

Associations between the average blastomere number of all embryos on day 3 and the live birth rate in the transfer cycle: a single-centre, retrospective study

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