Increased apoptosis and abnormal visual behavior by histone modifications with exposure to para-xylene in developing Xenopus

Gao, Jun; Ruan, Hangze; Qi, Xianjie; Guo, Xia; Zheng, Jingna; Liu, Cong; Fang, Yanxiao; Huang, Minjiao; Xu, Miao; Shen, Wanhua

doi:10.1016/j.neuroscience.2016.06.027

Cited by 14 publications

(7 citation statements)

References 40 publications

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“…(C)]. To test HDAC1‐GFP or HDAC1‐MO transfection will result in cell death, we performed TUNEL experiments as described before (Gao et al, ). We found that either knockdown or overexpression of HDAC1 did not induce cell apoptosis in the tectal cells (Supporting Information Fig.…”

Section: Resultsmentioning

confidence: 99%

Visual experience dependent regulation of neuronal structure and function by histone deacetylase 1 in developing Xenopus tectum in vivo

Ruan

Gao

et al. 2017

Developmental Neurobiology

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Histone deacetylase 1 (HDAC1) is thought to play pivotal roles in neurogenesis and neurodegeneration. However, the role of HDAC1 in neuronal growth and structural plasticity in the developing brain in vivo remains unclear. Here, we show that in the optic tectum of Xenopus laevis, HDAC1 knockdown dramatically decreased the frequency of AMPAR-mediated synaptic currents and increased the frequency of GABAAR-mediated currents, whereas HDAC1 overexpression significantly decreased the frequency of GABAAR-mediated synaptic currents. Both HDAC1 knockdown and overexpression adversely affected dendritic arbor growth and visual experience-dependent structural plasticity. Furthermore, HDAC1 knockdown decreased BDNF expression via a mechanism that involves acetylation of specific histone H4 residues at lysine K5. In particular, the deficits in dendritic growth and visually guided avoidance behavior in HDAC1-knockdown tadpoles could be rescued by acute tectal infusion of BDNF. These results establish a relationship between HDAC1 expression, histone H4 modification and BDNF signaling in the visual-experience dependent regulation of dendritic growth, structural plasticity and function in intact animals in vivo. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 77: 947-962, 2017.

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Section: Resultsmentioning

confidence: 99%

Visual experience dependent regulation of neuronal structure and function by histone deacetylase 1 in developing Xenopus tectum in vivo

Ruan

Gao

et al. 2017

Developmental Neurobiology

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“…In D. melanogaster in particular, exposure to benzene, xylene and toluene induced a delay in the number of flies that emerged into adult (Singh et al, 2009). In Xenopus laevis, treatment of tadpoles with p-xylene resulted in a significantly higher mortality, malformed tadpoles and developmental delay, in embryonic toxicity studies (Gao et al, 2016). In humans, there is also a relationship between high concentration of BTEX and neural tube defects in pregnant women (Lupo et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Toxicological Study and Genetic Basis of BTEX Susceptibility in Drosophila melanogaster

2020

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Benzene, toluene, ethylbenzene and xylene, also known as BTEX, are released into environmental media by petroleum product exploratory and exploitative activities and are harmful to humans and animals. Testing the effects of these chemicals on a significantly large scale requires an inexpensive, rapidly developing model organism such as Drosophila melanogaster. In this study, the toxicological profile of benzene, toluene, ethylbenzene, p-xylene, m-xylene, and o-xylene in D. melanogaster was evaluated. Adult animals were monitored for acute toxicity effects. Similarly, first instar larvae reared separately on the same compounds were monitored for the ability to develop into adult flies (eclosion). Further, the impact of fixed concentrations of benzene and xylene on apoptosis and mitosis were investigated in adult progenitor tissues found in third instar larvae. Toluene is the most toxic to adult flies with an LC 50 of 0.166 mM, while a significant and dose-dependent decrease in fly eclosion was observed with benzene, p-xylene, and o-xylene. An increase in apoptosis and mitosis was also observed in animals exposed to benzene and p-xylene. Through Genome Wide Association Screening (GWAS), 38 regions of the D. melanogaster genome were identified as critical for responses to p-xylene. This study reveals the strength of D. Melanogaster genetics as an accessible approach to study BTEX compounds.

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“…Neurotoxicity and hepatotoxicity of xylene is a result of the induction of apoptosis and the generation of reactive oxygen species [14]. It was shown that antioxidants prevent the oxidative effects of xylene in the central nervous system in animals [15]. Revilla et al [16] demonstrated that xylene cell toxicity is a result of mitochondrial uncoupling via ATP depletion and mitochondrial reactive oxygen species generation.…”

Section: Discussionmentioning

confidence: 99%

Toxicity of Insecticide Carrier Solvent: Effect of Xylene on Hemolymph Biochemical Parameters in Blaberus giganteus L.

Maliszewska¹,

Tęgowska²

2018

Pol. J. Environ. Stud.

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Xylene is an aromatic hydrocarbon occurring naturally in petroleum, coal, and wood tar. It is one of the top 30 chemicals produced in the United States in terms of volume. The annual production of xylene is estimated to be 16 million tons. Xylene is widely used as a solvent for paints, lacquers, glues, and pesticides, as well as in histological laboratories. Xylene is also found in airplane fuel, gasoline, and cigarette smoke [1-2]. In Poland, the threshold limit value for workers is 100 mg/m 3. It was estimated that hospital laboratory workers may be exposed to xylene in concentrations reaching 1,740 mg/m 3 , while printing house workers may be exposed to xylene in concentrations of about 130 mg/m 3 [3]. One can also come into contact with xylene through cigarette smoke, paints, automobile exhaust, and a variety of other consumer products [2]. Commercial products contain non-insecticidal ingredients that act as adjuvants, solvents, emulsifiers, or preservatives and which are not needed to be identified on the product label unless classified as highly toxic [4]. Many insecticide formulations contain 25 to 75 percent of solvent mixtures, and xylene and other aromatic hydrocarbons are frequently employed as solvents in pesticide concentrates [5]. Brattsten and Wilkinson [6] in experiments performed on southern armyworm demonstrated that, paradoxically, the carrier solvent in which the insecticide is formulated may aid in the

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Increased apoptosis and abnormal visual behavior by histone modifications with exposure to para-xylene in developing Xenopus

Cited by 14 publications

References 40 publications

Visual experience dependent regulation of neuronal structure and function by histone deacetylase 1 in developing Xenopus tectum in vivo

Visual experience dependent regulation of neuronal structure and function by histone deacetylase 1 in developing Xenopus tectum in vivo

Toxicological Study and Genetic Basis of BTEX Susceptibility in Drosophila melanogaster

Toxicity of Insecticide Carrier Solvent: Effect of Xylene on Hemolymph Biochemical Parameters in Blaberus giganteus L.

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