Increased angiogenic factors in exhaled breath condensate of children with severe asthma – New markers of disease progression?

Grzela, Katarzyna; Litwiniuk, Małgorzata; Krejner, Alicja; Zagórska, Wioletta; Grzela, Tomasz

doi:10.1016/j.rmed.2016.08.005

Cited by 9 publications

(15 citation statements)

References 11 publications

(12 reference statements)

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“…Hypoxia as well as inflammation are also relevant to asthma -therefore the biological properties of VEGF have led to interest of this molecule within the lung, suggesting that the higher expression of VEGF may play a potential role in the pathogenesis of asthma and lung remodeling. [13][14][15] Vascular endothelial growth factor can promote proliferation of airway smooth muscle (ASM) cells. It upregulates disintegrin and metalloproteinase (ADAM-33) mRNA and protein levels in a doseand time-dependent manner as well as phosphorylation of phospho-extracellular signal-regulated kinase 1/2 (ERK1/2) and phospho-Akt (Akt).…”

Section: Introductionmentioning

confidence: 99%

VEGF serum concentration and irreversible bronchoconstriction in adult asthmatics

Gomułka¹,

Liebhart²,

Gładysz³

et al. 2019

Adv Clin Exp Med

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Background. Vascular endothelial growth factor (VEGF) is an angiogenic, heparin-binding glycoprotein playing an important role in the pathogenesis of many diseases and disorders, including asthma. It has been reported that increased VEGF serum concentration is a biomarker of neovascularization, which could suggest that higher VEGF expression may be relevant to asthmatics with airway remodeling and irreversible bronchoconstriction. Objectives. The aim of this study was to assess the possible association between VEGF serum concentration and irreversible bronchoconstriction in adult patients with a diagnosis of asthma. Material and methods. This study involved 82 adult patients with asthma (42 persons with and 40 persons without irreversible bronchoconstriction) and 40 healthy adult controls. Vascular endothelial growth factor serum concentration was analyzed using enzyme-linked immunosorbent assay (ELISA). Results. Vascular endothelial growth factor serum concentration in patients with asthma was higher than in healthy controls (p = 0.0131), particularly in those from the subgroup of irreversible bronchoconstriction (p = 0.0133). The rising tendency was confirmed using the Kruskal-Wallis rank sum test that showed a significant difference (p = 0.0374) in VEGF values among the 3 groups examined: healthy controls (Me = 246.6 pg/mL), asthmatics with reversible bronchoconstriction (Me = 288.6 pg/mL) and asthmatics with irreversible bronchoconstriction (Me = 340.6 pg/mL). However, the direct comparison between the 2 asthmatics groups (reversible vs irreversible bronchoconstriction) did not show a statistically significant difference (p = 0.5521). Conclusions. Increased VEGF serum concentration is characteristic of patients with asthma, especially those with irreversible bronchoconstriction.

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Section: Introductionmentioning

confidence: 99%

VEGF serum concentration and irreversible bronchoconstriction in adult asthmatics

Gomułka¹,

Liebhart²,

Gładysz³

et al. 2019

Adv Clin Exp Med

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“…Asthma severity has been highly correlated with airway remodeling in the airway tract; these changes include extracellular matrix enhancement, smooth muscle cell hyperplasia, and neovascularization (47). Proangiogenic factors play important roles in asthma progression.…”

Section: Angiogenic Factorsmentioning

confidence: 99%

“…Elevated PDGF-AA levels are observed in the EBC of asthmatic children with severe airflow limitation. PDGF plays an important role in bronchial fibrosis (47,48).…”

Section: Angiogenic Factorsmentioning

confidence: 99%

Role of Cytokines and Chemokines in the Outcome of Children With Severe Asthma

Hatami

Ghaffari

et al. 2018

JPR

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Context: Asthma, characterized by airway inflammation, is a common chronic disease of childhood. Cytokines and chemokines could be used in the diagnosis, treatment, and management of asthma severity in children. In this review, we have explained the application of cytokines and chemokines as biomarkers in pediatric asthma. Evidence Acquisition: All related articles were separately searched by two researchers using the following keywords in PubMed, Scopus, and Embase databases: Cytokine biomarkers, chemokines biomarkers, and children asthma. Articles published from 2000 to 2017 were investigated in the research, and 28 articles were included in the final analysis for this review. Results: About cytokines, serum Interleukin 4 (IL-4) level is a marker of the presence of asthma, and IL-13 is a key cytokine involved in the manifestation of asthma symptoms. High IL-13 concentration and number of IL-13+ cells in the bronchial submucosa specimens are characteristic of severe asthma. Serum IL-5 concentration 3.1 times in children with severe asthma. IL-17 is involved in airway obstruction. IFN-γ gene polymorphism (+874A/T) in children elevates susceptibility to asthma. TGFB1 polymorphisms are considered as indicators of asthma severity. IL-26 plays an important role in asthma severity. IP-10 may be a useful inflammatory marker of asthma severity. High periostin level has been identified in pediatric asthma. PDGF level, which is high in asthma patients, plays an important role in bronchial fibrosis. About chemokines, plasma TARC concentration may be a useful biomarker of airway inflammation and asthma severity in children. Studies have supported the association between high serum RANTES levels and severe airway obstruction in children. CXCR4 levels are high in pediatric asthma and are associated with disease severity. Conclusions: A wide range of cytokines and chemokines may play important roles in asthma severity in pediatric patients. Therefore, several studies have recommended the use of multiple molecular biomarkers, such as cytokines, for determining asthma severity in children.

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“…In a recent study we have demonstrated that EBC samples contained significant quantities of several angiogenesis regulators [4]. Moreover, it was suggested that the amount of angiogenesis-related factors in EBC as well as vascular density of the airway wall may correlate with asthma severity [1,4].…”

Section: Introductionmentioning

confidence: 99%

“…Elevated levels of angiogenic factors in the bronchial wall of asthmatic patients have been shown in several studies [1,3]. The presence of the angiogenesis-related factors was investigated also in exhaled breath condensates (EBCs) of children with asthma [4].…”

Section: Introductionmentioning

confidence: 99%

Influence of exhaled breath condensates from children with asthma on endothelial cells cultured in vitro. Do we really know everything about our breath condensate?

Krejner‐Bienias

Grzela

Zagórska

et al. 2020

pdia

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Introduction: Asthma-associated remodelling involves subepithelial fibrosis and increased vascularization of the bronchial wall. The latter may be associated with excessive production of several angiogenesis regulators which may be found in exhaled breath condensates (EBCs) collected from children with asthma. Aim: To assess the influence of EBC samples of asthmatic children and healthy controls on in vitro cultures of normal human lung microvascular endothelial cells (HLMVEC) and murine endothelial cell line (C-166). Moreover, the proteomic profile of cytokines in EBC samples was analysed. Material and methods: Breath condensates collected from children with mild asthma (n = 10) and from healthy controls (n = 10) were used for experiments. Colorimetric tetrazolium salt reduction assay was used to evaluate the effect of EBCs on HLMVEC and C-166 cell lines. Furthermore, influence of EBCs on C-166 cell line was assessed using Annexin V-binding assay. The cytokine screening of EBC samples was performed using a proteome microarray system. Results: The EBCs from patients with asthma revealed a weak inhibitory influence on human and murine endothelial cells. Surprisingly, EBCs from healthy children led to cell death, mainly by the induction of apoptosis. There were no statistically significant differences in the cytokine profile between EBC samples from children with asthma and healthy controls. Conclusions: Our preliminary report shows for the first time that the incubation of EBCs from healthy controls induced apoptosis in endothelial cells. The detailed mechanism responsible for this action remains unknown and requires further research.

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Increased angiogenic factors in exhaled breath condensate of children with severe asthma – New markers of disease progression?

Cited by 9 publications

References 11 publications

VEGF serum concentration and irreversible bronchoconstriction in adult asthmatics

VEGF serum concentration and irreversible bronchoconstriction in adult asthmatics

Role of Cytokines and Chemokines in the Outcome of Children With Severe Asthma

Influence of exhaled breath condensates from children with asthma on endothelial cells cultured in vitro. Do we really know everything about our breath condensate?

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