Increased Angiogenesis and Lymphangiogenesis in the Placental Villi of Women with Chronic Venous Disease during Pregnancy

Ortega, Miguel Á.; Sáez, Miguel A.; Fraile-Martínez, Óscar; Asúnsolo, Ángel; Pekarek, Leonel; Bravo, Coral; Coca, Santiago; Sáinz, Felipe; Álvarez-Mon, Melchor; Buján, Julia; García‐Honduvilla, Natalio

doi:10.3390/ijms21072487

Cited by 26 publications

(34 citation statements)

References 41 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CVD is a frequent complication of pregnancy, that can alter placental homeostasis and have important repercussions on the health of the mother and the fetus ( 3 , 19 ). The ECM is a dynamic network of macromolecules, that is constantly being remodeled ( 29 , 30 ).…”

Section: Discussionmentioning

confidence: 99%

“…Alterations in the composition of the ECM are present in a wide variety of pathologies associated with pregnancy, such as preeclampsia and gestational trophoblastic diseases ( 16 ). Similarly, the placenta of patients with CVD during pregnancy was found to have significant alterations in the composition of the ECM, such as in the collagen fibers or in the calcifications of the placental villi ( 17 – 19 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level

et al. 2021

Self Cite

View full text Add to dashboard Cite

Chronic venous disease (CVD) is the response to a series of hemodynamic changes in the venous system and the onset of this disease is often triggered by pregnancy. Placental tissue is particularly sensitive to the characteristic changes which occurs in venous hypertension. In this regard, changes in the extracellular matrix (ECM), that occur to adapt to this situation, are fundamental to controlling elastogenesis. Therefore, the aim of the present study was to analyze the changes that occur in the mRNA and protein expression level of proteins related to elastogenesis in the placental villi of women diagnosed with CVD, in the third trimester of pregnancy. An observational, analytical and prospective cohort study was conducted, in which the placenta from 62 women with CVD were compared with that in placenta from 52 women without a diagnosis of CVD. Gene and protein expression levels were analyzed using reverse transcription-quantitative PCR and immunohistochemistry, respectively. The results showed a significant decrease in the gene and protein expression level of EGFL7 in the placental villi of women with CVD. By contrast, significant increases in the gene and protein expression level of ECM-related proteins, such as tropoelastin, fibulin 4, fibrillin 1 and members of the lysyl oxidase family (LOX and LOXL-1) were also found in the placental villi of women with CVD. To the best of our knowledge, the results from the present study showed for the first time that CVD during pregnancy was associated with changes in the mRNA and protein expression level in essential components of the EGFL7-modulated elastogenesis process in placental villi.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Antigen-antibody reactions were detected by the ABC method 45 , 46 (avidin-biotin complex) using peroxidase or alkaline phosphatase as a chromogen according to the following protocol. 1.…”

Section: Methodsmentioning

confidence: 99%

Histopathological study of JNK in venous wall of patients with chronic venous insufficiency related to osteogenesis process

Ortega¹,

Asúnsolo²,

Pekarek³

et al. 2021

Int. J. Med. Sci.

Self Cite

View full text Add to dashboard Cite

Chronic venous insufficiency (CVI) is one of the most common vascular pathologies worldwide. One of the risk factors for the development of CVI is aging, which is why it is related to senile changes. The main trigger of the changes that occur in the venous walls in CVI is blood flow reflux, which produces increased hydrostatic pressure, leading to valve incompetence. The cellular response is one of the fundamental processes in vascular diseases, causing the activation of cell signalling pathways such as c-Jun N-terminal kinase (JNK). Metabolic changes and calcifications occur in vascular pathology as a result of pathophysiological processes. The aim of this study was to determine the expression of JNK in venous disease and its relationship with the role played by the molecules involved in the osteogenic processes in venous tissue calcification. This was a cross-sectional study that analyzed the greater saphenous vein wall in 110 patients with (R) and without venous reflux (NR), classified according to age. Histopathological techniques were used and protein expression was analysed using immunohistochemistry techniques for JNK and markers of osteogenesis (RUNX2, osteocalcin (OCN), osteopontin (OPN)). Significantly increased JNK, RUNX2, OCN, OPN and pigment epithelium-derived factor (PEDF) protein expression and the presence of osseous metaplasia and amorphous calcification were observed in younger patients (<50 years) with venous reflux. This study shows for the first time the existence of an osteogenesis process related to the expression of JNK in the venous wall.

show abstract

“…It is a condition of great prevalence, more common amongst women and people aged 40-80 years, with an estimated annual incidence of 2% [ 2 ]. Amongst the risk factors associated with chronic venous disease, family history, aging, obesity, sedentarism, pregnancy, hormonal factors, and greater height are of great significance [ 3 , 4 ]. Furthermore, CVeD implies a great cost to the health system, especially in occidental societies [ 3 ]; consequently, it is necessary to keep expanding the knowledge of this disease towards a more appropriate management.…”

Section: Introductionmentioning

confidence: 99%

Chronic Venous Disease Patients Showed Altered Expression of IGF-1/PAPP-A/STC-2 Axis in the Vein Wall

Ortega

Fraile-Martínez

Asúnsolo

et al. 2020

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Chronic venous disease (CVeD) has a remarkable prevalence, with an estimated annual incidence of 2%. It has been demonstrated how the loss of homeostatic mechanisms in the vein wall can take part in the physiopathology of CVeD. In this regard, it has been described how different axis, such as IGF-1/PAPP-A/STC-2 axis, may play an essential role in tissue homeostasis. The aim of this research is to study both genetic and protein expressions of the IGF-1/PAPP-A/STC-2 axis in CVeD patients. It is a cross-sectional study in which genetic (RT-qPCR) and protein (immunohistochemistry) expression analysis techniques were accomplished in saphenous veins from CVeD patients ( n = 35 ) in comparison to individuals without vascular pathology (HV). Results show a significant increase in both genetic and protein expressions of PAPP-A and IGF-1, and a decrement STC-2 expression at the same time in CVeD patients. Our study is a pioneer for demonstrating that the expression of the different components of the IGF-1/PAPP-A/STC-2 axis is altered in CVeD patients. This fact can be a part of the loss of homeostatic mechanisms of the venous tissue. Further research should be destined to deepen into alterations of this axis, as well as to evaluate the usage of these components as therapeutic targets for CVeD.

show abstract

Increased Angiogenesis and Lymphangiogenesis in the Placental Villi of Women with Chronic Venous Disease during Pregnancy

Cited by 26 publications

References 41 publications

An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level

An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level

Histopathological study of JNK in venous wall of patients with chronic venous insufficiency related to osteogenesis process

Chronic Venous Disease Patients Showed Altered Expression of IGF-1/PAPP-A/STC-2 Axis in the Vein Wall

Contact Info

Product

Resources

About