Increased abundance of ADAM9 transcripts in the blood is associated with tissue damage

Rinchai, Darawan; Kewcharoenwong, Chidchamai; Kessler, Bianca; Lertmemongkolchai, Ganjana; Chaussabel, Damien

doi:10.12688/f1000research.6241.2

Cited by 12 publications

(12 citation statements)

References 40 publications

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“…It has been proposed as a biomarker for tissue damage and shown to be of particular use in excluding a confounding effect of an infection, as in vitro exposure to pathogen-associated molecular patterns (PAMPs) leads to a very limited change in the expression of ADAM9, in contrast to many other biomarkers. [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Hemorrhagic Shock Induces a Rapid Transcriptomic Shift of the Immune Balance in Leukocytes after Experimental Multiple Injury

Debler

Palmer

Braumüller

et al. 2021

Mediators of Inflammation

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The immune response following trauma represents a major driving force of organ dysfunction and poor outcome. Therefore, we investigated the influence of an additional hemorrhagic shock (HS) on the early posttraumatic immune dysbalance in the whole population of blood leukocytes. A well-established murine polytrauma (PT) model with or without an additional pressure-controlled HS (mean arterial pressure of 30 mmHg (±5 mmHg) for 60 mins, afterwards fluid resuscitation with balanced electrolyte solution four times the volume of blood drawn) was used. C57BL/6 mice were randomized into a control, PT, and PT + HS group with three animals in each group. Four hours after trauma, corresponding to three hours after induction of hemorrhage, RNA was isolated from all peripheral blood leukocytes, and a microarray analysis was performed. Enrichment analysis was conducted on selected genes strongly modulated by the HS. After additional HS in PT mice, the gene expression of pathways related to the innate immunity, such as IL-6 production, neutrophil chemotaxis, cell adhesion, and toll-like receptor signaling was upregulated, whereas pathways of the adaptive immune system, such as B- and T-cell activation as well as the MHC class II protein complex, were downregulated. These results demonstrate that an additional HS plays an important role in the immune dysregulation early after PT by shifting the balance to increased innate and reduced adaptive immune responses.

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Section: Discussionmentioning

confidence: 99%

Hemorrhagic Shock Induces a Rapid Transcriptomic Shift of the Immune Balance in Leukocytes after Experimental Multiple Injury

Debler

Palmer

Braumüller

et al. 2021

Mediators of Inflammation

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“…In May of 2017, 2004 papers about TKT were returned after running the query: TKT OR transketolase OR HEL107 OR TK1 . Candidates for follow on investigation among the 24 NET-associated proteins were selected based on under-representation among the NET literature (exposing the existence of a knowledge gap) [24]. No overlap was found between the TKT and NET literature: TKT OR transketolase OR HEL107 OR TK1 AND “Neutrophil extracellular traps” (S1 Table).…”

Section: Methodsmentioning

confidence: 99%

Transketolase and vitamin B1 influence on ROS-dependent neutrophil extracellular traps (NETs) formation

et al. 2019

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Neutrophil extracellular traps (NETs) are a recently identified, web-like, extracellular structure composed of decondensed nuclear DNA and associated antimicrobial granules. NETs are extruded into the extracellular environment via the reactive oxygen species (ROS)-dependent cell death pathway participating in inflammation and autoimmune diseases. Transketolase (TKT) is a thiamine pyrophosphate (vitamin B1)-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway by feeding excess sugar phosphates into the main carbohydrate metabolic pathways to generate biosynthetic reducing capacity in the form of NADPH as a substrate for ROS generation. In this work, TKT was selected as a lead candidate from 24 NET-associated proteins obtained by literature screening and knowledge gap assessment. Consequently, we determined whether TKT influenced NET formation in vitro . We firstly established that the release of ROS-dependent NETs was significantly decreased after purified human PMNs were pretreated with oxythiamine, a TKT inhibitor, and in a concentration dependent manner. As a cofactor for TKT reaction, we evaluated the release of NET formation either in vitamin B1 treatment or in combined use of oxythiamine and vitamin B1, and found that those treatments also exerted a significant suppressive effect on the amount of NET-DNA and ROS production. The regulation of TKT by oxythiamine and/or vitamin B1 may therefore be associated with response to the modulation of NET formation by preventing generation of excessive NETs in inflammatory diseases.

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“…It consists of determining whether the knowledge conveyed by the data being examined is novel, and if the answer is yes, then to gain a measure of its potential impact or significance. A proof of principle study was published and can be consulted to illustrate the steps described below …”

Section: Cod1: Reductionist Investigations Using Collective Omics Datamentioning

confidence: 99%

“…A proof of principle was recently obtained with one trainee completing all three modules . Five other scientists having completed at least one training module …”

Section: Introductionmentioning

confidence: 99%

Using ‘collective omics data’ for biomedical research training

Chaussabel

Rinchai

2018

Immunology

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Summary Systems‐scale molecular profiling data accumulating in public repositories may constitute a useful resource for immunologists. It is for instance likely that information relevant to their chosen line of research be found among the more than 90,000 data series available in the NCBI Gene Expression Omnibus. Such ‘collective omics data’ may also be employed as source material for training purposes. This is the case when training curricula aim at the development of bioinformatics skills necessary for the analysis, interpretation or visualization of data generated on global scales. But ‘collective omics data’ may also be reused for training purposes to foster the development of the skills and ‘mental habits’ underpinning traditional reductionist science approaches. This review describes a small‐scale initiative involving investigators, for the most part immunologists, having engaged in a range of training activities relying on ‘collective omics data’.

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Increased abundance of ADAM9 transcripts in the blood is associated with tissue damage

Cited by 12 publications

References 40 publications

Hemorrhagic Shock Induces a Rapid Transcriptomic Shift of the Immune Balance in Leukocytes after Experimental Multiple Injury

Hemorrhagic Shock Induces a Rapid Transcriptomic Shift of the Immune Balance in Leukocytes after Experimental Multiple Injury

Transketolase and vitamin B1 influence on ROS-dependent neutrophil extracellular traps (NETs) formation

Using ‘collective omics data’ for biomedical research training

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