Increase of the phytase production by Aspergillus japonicus and its biocatalyst potential on chicken feed treatment

Abstract

Phytase hydrolyzes phytic acid from the plant components of animal feed, releasing inorganic phosphorus. The phytase production by Aspergillus japonicus was optimized using Plackett-Burman designs (PBD), composite central rotational designs (CCRD), and response surface methodology from standard Czapek medium. The enzyme was applied in broiler chicken and laying hen foods. Analysis from PBD showed that KH2 PO2, MgSO4 · 7H2O, and yeast extract had significant influences on phytase secretion (p < 0.05). The best results from the CCRD experiments were obtained using (A) 0.040% KH2 PO4, (B) 0.050% MgSO4 · 7H2O, and (C) 0.040% yeast extract, enhancing in 49-53 U mg(-1) protein. The determination coefficient (R(2)) was 0.92 and Fcalc was 7.48 times greater than Flisted . Thus, the reduced coded model: Y (U mg-1) = 50.29 + 4.30A - 3.35(A)2 - 4.80(B)2 + 5.62C - 4.26(C)2 was considered predictive and statistically significant (p < 0.05). The optimized culture medium increased the phytase yield in 250%. A. japonicus phytase released high levels of Pi from broiler chicken and laying hen food. A. japonicus is an excellent phytase producer in a culture medium using inexpensive components and agricultural wastes. Therefore, these results provide sound arguments for the formulation of a low cost culture medium for phytase production.

Mixed Substrate Fermentation for Enhanced Phytase Production by Thermophilic Mould Sporotrichum thermophile and Its Application in Beneficiation of Poultry Feed

“…However, there was more inorganic phosphorus liberation from laying hens' feed as compared to broiler chicks' feed. This is due to the requirement of higher phosphorus diet by the laying hens [13]. The phytase has an immense biotechnological application in improving the availability of inorganic phosphorus, soluble protein and reducing sugars which would be efficiently utilized by the poultry birds.…”

“…The phytase has an immense biotechnological application in improving the availability of inorganic phosphorus, soluble protein and reducing sugars which would be efficiently utilized by the poultry birds. Similarly, A. japonicus phytase released inorganic phosphate from broiler chick and laying hen feeds [13]. The use of S. thermophile phytase in poultry feed will reduce the requirement of external phosphorus supplementation in the diets of poultry birds and other monogastrics, thus making the bioavailability of other nutritional components.…”

High levels of β-xylosidase in Thermomyces lanuginosus : potential use for saccharification

“…Successful strategies for the production of hemicellulases with biotech traits not only depend on the selection of new microorganisms but also on the improvement of experimental strategies that take maximum advantage of its potential. 18 , 19 …”

Biochemical effect of a histidine phosphatase acid (phytase) of Aspergillus japonicus var. Saito on performance and bony characteristics of broiler

“…Phytase activity was determined using ammonium molybdate method (Yin et al 2007) modified by Maller et al (2014), using as substrate 10 mg/mL phytic acid dodecasodium (Sigma-Aldrich, St. Louis, MO). A standard curve was generated using 100–1000 µM KH 2 PO 4 solutions.…”

“…These conditions are found in digestive tract of broiler and indicate a good performance of this enzyme in supplementation of animal feed. In this way, previous works showed positive effects in vitro of the supplementation of this enzyme on broiler food (Promdonkoy et al 2009; Maller et al 2014), but no works were found in the literature about effects in vivo.…”