Increase of serum phosphatidylcholine hydroperoxide dependent on glycemic control in type 2 diabetic patients

Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetes

“…As mentioned in the introduction, we previously confirmed that plasma PCOOH increases in type 2 diabetes patients and that the concentration is proportional to Hb A1c (4). Our subsequent study demonstrated that Amadori-PE is capable of reactive oxygen species generation and thereby triggers lipid peroxidation (7).…”

“…To determine lipid hydroperoxides as a primary oxidation product, we established the chemiluminescence detection-liquid chromatography method (2). Using this method, it was confirmed that plasma phosphatidylcholine hydroperoxide (PCOOH) increases in hyperlipidemic patients (3) and in type 2 diabetic patients (4). Hence, we hypothesized that plasma PCOOH formation is closely involved in the pathophysiology of these diseases.…”

High-fat Diet Increases Phospholipid Peroxidation in the Liver of Mature Fischer 344 Rats

“…1 , and thereby, it was confirmed that phospholipid peroxidation can be accelerated under the conditions used in this study, even without the administration of chemicals such as carbon tetrachloride. The PCOOH level in mature rats was approximately one and a half times higher than that in the juvenile group; this increase in PCOOH was almost equivalent to that found in the plasma PCOOH of hyperlipidemic patients 2 and diabetic patients 5 .…”

Fructose Ingestion Enhances Atherosclerosis and Deposition of Advanced Glycated End-products in Cholesterol-fed Rabbits

“…It has been shown that fructose administration increased glycation and produced AGEs (17)(18)(19)(20), and the process could trigger lipid peroxidation (26,27). In our previous study, serum PCOOH levels increased in diabetic patients and this increase correlated with the level of HbA1c, a marker of glycation (13). The increase in PCOOH levels in cholesterol-fed rabbits (CN group) would be exaggerated by glycation induced by fructose ingestion as shown in the CF group.…”

“…Phospholipids coat the surface of lipoproteins and may be the initial target of oxidative stress reactions causing lipid peroxidation and subsequent hyperlipidemia. High plasma PCOOH levels were found in diabetic patients even in those without hyperlipidemia and vascular complications (13). It is thought that hyperglycemia is related to oxidative stress (14) via glycation (15,16).…”