Increase of organic solvent tolerance by overexpression of manXYZ in Escherichia coli

Okochi, Mina; Kurimoto, Masaki; Shimizu, Kazunori; Honda, Hiroyuki

doi:10.1007/s00253-006-0624-y

Cited by 44 publications

(32 citation statements)

References 23 publications

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“…The manXYZ operon, encoding a phosphotransferase system (PTS) transporter, was downregulated Ͼ2-fold under chlorine treatment in both S. enterica strains. The expression of this operon in E. coli was found to enhance bacterial tolerance to organic solvents (47). Meanwhile, genes encoding the PTS in S. enterica, such as ptsG and ptsHI, were also significantly downregulated.…”

Section: Resultsmentioning

confidence: 96%

Transcriptomic Responses of Salmonella enterica Serovars Enteritidis and Typhimurium to Chlorine-Based Oxidative Stress

Wang

Phillippy

Deng

et al. 2010

Appl Environ Microbiol

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Salmonella enterica serovars Enteritidis and Typhimurium are the leading causative agents of salmonellosis in the United States. S. Enteritidis is predominantly associated with contamination of shell eggs and egg products, whereas S. Typhimurium is frequently linked to tainted poultry meats, fresh produce, and recently, peanut-based products. Chlorine is an oxidative disinfectant commonly used in the food industry to sanitize the surfaces of foods and food processing facilities (e.g., shell eggs and poultry meats). However, chlorine disinfection is not always effective, as some S. enterica strains may resist and survive the disinfection process. To date, little is known about the underlying mechanisms of how S. enterica responds to chlorine-based oxidative stress. In this study, we designed a custom bigenome microarray that consists of 385,000 60-mer oligonucleotide probes and targets 4,793 unique gene features in the genomes of S. Enteritidis strain PT4 and S. Typhimurium strain LT2. We explored the transcriptomic responses of both strains to two different chlorine treatments (130 ppm of chlorine for 30 min and 390 ppm of chlorine for 10 min) in brain heart infusion broth. We identified 209 S. enterica core genes associated with Fe-S cluster assembly, cysteine biosynthesis, stress response, ribosome formation, biofilm formation, and energy metabolism that were differentially expressed (>1.5-fold; P < 0.05). In addition, we found that serovars Enteriditis and Typhimurium differed in the responses of 33 stress-related genes and 19 virulence-associated genes to the chlorine stress. Findings from this study suggest that the oxidative-stress response may render S. enterica resistant or susceptible to certain types of environmental stresses, which in turn promotes the development of more effective hurdle interventions to reduce the risk of S. enterica contamination in the food supply.

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Section: Resultsmentioning

confidence: 96%

Transcriptomic Responses of Salmonella enterica Serovars Enteritidis and Typhimurium to Chlorine-Based Oxidative Stress

Wang

Phillippy

Deng

et al. 2010

Appl Environ Microbiol

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“…and releases the phosphate esters into the cytoplasm (36). The manXYZ system has also been implicated in solvent tolerance in E. coli (33). Interestingly, another upregulated protein, MgsA (methylglyoxal synthase), limits the accumulation of phosphorylated sugars and has been postulated to provide a bypass mechanism for triose phosphate metabolism during growth in inadequate inorganic phosphate levels (47).…”

Section: Resultsmentioning

confidence: 99%

Functional Genomic Study of Exogenous n -Butanol Stress in Escherichia coli

Rutherford

Dahl

Price

et al. 2010

Appl Environ Microbiol

214

258

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n-Butanol has been proposed as an alternative biofuel to ethanol, and several industrially used microbes, including Escherichia coli, have been engineered to produce it. Unfortunately, n-butanol is more toxic than ethanol to these organisms. To understand the basis for its toxicity, cell-wide studies were conducted at the transcript, protein, and metabolite levels to obtain a global view of the n-butanol stress response. Analysis of the data indicates that n-butanol stress has components common to other stress responses, including perturbation of respiratory functions (nuo and cyo operons), oxidative stress (sodA, sodC, and yqhD), heat shock and cell envelope stress (rpoE, clpB, htpG, cpxR, and cpxP), and metabolite transport and biosynthesis (malE and opp operon). Assays using fluorescent dyes indicated a large increase in reactive oxygen species during n-butanol stress, confirming observations from the microarray and proteomics measurements. Mutant strains with mutations in several genes whose products changed most dramatically during n-butanol stress were examined for increased sensitivity to n-butanol. Results from these analyses allowed identification of key genes that were recruited to alleviate oxidative stress, protein misfolding, and other causes of growth defects. Cellular engineering based on these cues may assist in developing a high-titer, n-butanol-producing host.

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“…The PTS system is responsible for the uptake of carbohydrates and includes the mannose-specific IIC component, which can catalyse mannose to mannose 6-phosphate, during uptake (Okochi et al, 2007). Sucrose was also metabolized by strain R201…”

mentioning

confidence: 99%

Description of Trichococcus ilyis sp. nov. by combined physiological and in silico genome hybridization analyses

Strepis

Sánchez‐Andrea

Gelder

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Increase of organic solvent tolerance by overexpression of manXYZ in Escherichia coli

Cited by 44 publications

References 23 publications

Transcriptomic Responses of Salmonella enterica Serovars Enteritidis and Typhimurium to Chlorine-Based Oxidative Stress

Transcriptomic Responses of Salmonella enterica Serovars Enteritidis and Typhimurium to Chlorine-Based Oxidative Stress

Functional Genomic Study of Exogenous n -Butanol Stress in Escherichia coli

Description of Trichococcus ilyis sp. nov. by combined physiological and in silico genome hybridization analyses

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