Increase of E3 ubiquitin ligase NEDD4 expression leads to degradation of its target proteins PTEN/IGF1R during the formation of goose fatty liver

Yan, Chunchi; Zhao, Minmeng; Li, Shuo; Liu, Tongjun; Xu, Cheng; Liu, Long; Geng, Tuoyu; Gong, Daoqing

doi:10.1093/jas/skaa270

Cited by 6 publications

(6 citation statements)

References 22 publications

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“…The seed node of module 2 is FBXO30 , which acts as an ubiquitin ligase. A recent study indicated that expression of protein ubiquitination in goose fatty liver was higher than in the normal liver ( Yan et al, 2020 ). This result appears to support other results regarding fatty liver development.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of the liver in aged laying hens with different eggshell strength

Han¹,

Kim²,

Kim³

et al. 2023

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of the liver in aged laying hens with different eggshell strength

Han¹,

Kim²,

Kim³

et al. 2023

Poultry Science

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“…No. P0010, Beyotime Biotechnology, Shanghai, China) as previously described (Yan et al, 2020). 2.6 | RNA isolation, cDNA synthesis and qPCR…”

Section: Oil Red O Stainingmentioning

confidence: 99%

“…Protein concentration of each tissue or cell sample was determined as previously described (Yan et al, 2020). The protocol for immunoblot analysis was also previously described (Geng, Xia, Russo, et al, 2015).…”

Section: Protein Assay and Immunoblot Analysismentioning

confidence: 99%

Glucose participates in the formation of goose fatty liver by regulating the expression of miRNA‐33/CROT

Lin

Xing

Zhang

et al. 2021

Animal Science Journal

Self Cite

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Glucose oversupply promotes formation of fatty liver, and fatty liver is usually accompanied with hyperglycemia. However, the mechanism by which glucose promotes formation of fatty liver is not very clear. In this study, fatty liver was successfully induced in Landes goose by 19 days of overfeeding with corn-based feed, the overfed geese had a significantly higher level of blood glucose than the normally fed geese (control group). In goose primary liver cells, high level of glucose promoted fat deposition and induced the expression of SREBF2(or SREBP2), a key regulator of lipid metabolism, and its intronic gene, miR-33. Moreover, overexpression of miRNA-33(miR-33) promotes lipid accumulation in goose primary liver cells. Consistently, miR-33 inhibitor suppressed glucose induced lipid accumulation in liver cells. Interestingly, the relative abundance of miR-33 in goose fatty liver was significantly higher than that in normal liver, while the relative mRNA and protein abundances of CROT, the target gene of miR-33, in goose fatty liver were significantly lower than those in goose normal liver. Taken together, these findings suggest that miR-33 mediates glucose promotion of lipid accumulation in goose primary liver cells, and that glucose participates in formation of goose fatty liver by regulating the expression of miR-33/CROT.

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“…Previous studies found that the expression level of circDock6 was downregulated in AML-12 cells, with the most signi cant increase in the mmu-let-7 g-5p expression level. Moreover, as the target of mmu-let-7 g-5p, IGF1R has been reported to be involved in metabolic homeostasis [13] and severe steatosis-associated injury [14].…”

Section: Introductionmentioning

confidence: 99%

CircDock6 promotes NAFLD by regulating IGF1R through targeting mmu-let-7 g-5p

Li,

Yu,

Ding

et al. 2023

Preprint

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Circular RNAs (circRNAs) are a novel kind of noncoding RNA with a closed ring structure. An increasing number of studies have shown that it plays an important role in NAFLD. In this study, we used circDock6 to explore its role and molecular mechanism in the progression of NAFLD. qRT‒PCR was used to detect the circDock6 expression level in SD liver tissues and HFD tissues. The regulatory effects of circDock6 in NAFLD were evaluated by Oil Red “O” staining, triglyceride detection, and western blot (WB) analysis. CircDock6 was significantly upregulated in HFD liver tissues compared to SD tissues. Additionally, Oil Red “O” staining and triglyceride detection revealed that decreased expression levels of circDock6 inhibited lipid droplet formation and reduced triglyceride content. CircDock6 acts as a mmu-let-7 g-5p sponge to promote the progression of NAFLD by regulating IGF1R. Our data demonstrated that circDock6 was expressed at high levels in HFD tissues. Knockdown of circDock6 expression inhibited NAFLD progression by regulating IGF1R through targeting mmu-let-7 g-5p.

show abstract

Increase of E3 ubiquitin ligase NEDD4 expression leads to degradation of its target proteins PTEN/IGF1R during the formation of goose fatty liver

Cited by 6 publications

References 22 publications

Transcriptomic analysis of the liver in aged laying hens with different eggshell strength

Transcriptomic analysis of the liver in aged laying hens with different eggshell strength

Glucose participates in the formation of goose fatty liver by regulating the expression of miRNA‐33/CROT

CircDock6 promotes NAFLD by regulating IGF1R through targeting mmu-let-7 g-5p

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