Increase of 5‐hydroxyindoleacetic acid and homovanillic acid in cisternal fluid of cats subjected to stress

Griauzde, Mindaugas Leon.; Radulovački, Miodrag

doi:10.1111/j.1471-4159.1976.tb07024.x

Cited by 14 publications

(4 citation statements)

References 19 publications

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“…Methods involving cerebrospinal fluid (CSF) sampling have been used extensively in humans (e.g. Garelis et al, 1974) and repetitive sampling of CSF has been used in studying transmitter metabolism in dogs (Guldberg and Yates, 1968), cats (Griauzde and Radulovacki, 1976) and primates (Baker and Ridley, 1979). The advent of high pressure liquid chromatography permits the application of similar methods to the much smaller volumes of CSF available from rats.…”

Section: Determination Of Brain 5-hydroxytryptamine Turnover In Freelmentioning

confidence: 99%

Determination of Brain 5‐Hydroxytryptamine Turnover in Freely Moving Rats Using Repeated Sampling of Cerebrospinal Fluid

Sarna¹,

Hutson²,

Tricklebank³

et al. 1983

Journal of Neurochemistry

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A simple technique is described for repeated sampling of cerebrospinal fluid (CSF) from the freely moving rat and its use in the determinations of 5-hydroxytryptamine (5-HT) turnover validated. A catheter, constructed from polyethylene tubing (PP10) was implanted via a cranial approach into the cisterna magna and x-ray studies confirmed that the catheter avoided the cerebellum. 5-HT turnover was determined from the rate of rise of 5-hydroxyindoleacetic acid (5-HIAA) in both CSF and brain following an injection of probenecid (200 mg/kg i.p.). Concentrations of 5-HIAA, 5-HT and tryptophan were determined by high pressure liquid chromatography. Turnover values for individual rats were obtained using CSF samples. After p-chlorophenylalanine treatment (when brain 5-HT was depleted by 43%) 5-HT turnover values obtained were comparably reduced whether determined from CSF (-67%) or brain (-74%). Thus differences of rat brain 5-HT turnover are proportionately reflected by CSF measurements. The method for sampling of CSF should be applicable in a wide range of pharmacological and physiological situations.

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Section: Determination Of Brain 5-hydroxytryptamine Turnover In Freelmentioning

confidence: 99%

Determination of Brain 5‐Hydroxytryptamine Turnover in Freely Moving Rats Using Repeated Sampling of Cerebrospinal Fluid

Sarna¹,

Hutson²,

Tricklebank³

et al. 1983

Journal of Neurochemistry

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“…Post et al (13) observed significant increases in concentrations of these metabolites when hospitalized, severely depressed patients were forced to exercise for 4 h prior to lumbar puncture (LP). Animal studies have documented increases in both brain and CSF concentrations of these metabolites following the induction of stress (14,15).…”

mentioning

confidence: 99%

Different neurotransmitter metabolite concentrations in CSF samples from inpatient and outpatient normal volunteers

Guthrie¹,

Berrettini²,

Rubinow³

et al. 1986

Acta Psychiatr Scand

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Cerebrospinal fluid concentrations of the neurotransmitter metabolites 5-hydroxyindoleacetic acid (5HIAA), homovanillic acid (HVA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylglycol (MHPG) were compared in two groups of healthy volunteer subjects. One group (outpatient) was composed of 27 subjects who were transported to the outpatient clinic on the day of the lumbar puncture (LP). The other group (inpatient) was composed of 10 subjects who were admitted to the NIMH Research Ward on the evening prior to the LP. After statistical adjustment for age, height, sex and season in which LP was performed the inpatient group had significantly higher concentrations of both 5HIAA and HVA (P less than 0.005 and P less than 0.05, respectively) than the outpatient group. The difference in DOPAC concentration approached significance (P = 0.056), but there was no difference in MHPG concentration between the groups. This result indicates the need for strict control of environment in studies of CSF monoamines and their metabolites.

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“…Four levels of time factor were present representing the time of day at which the samples were taken (8:30 a.m. until 2:30 p.m.). In addition we also tested the logarithmic transformation of each measure ment (10). Partial results of the ANOVA test are presented in terms of p-values in table I.…”

Section: Resultsmentioning

confidence: 99%

Effects of Diazepam on Sleep, Temperature, 5-Hydroxyindoleacetic and Homovanillic Acids in Cisternal Cerebrospinal Fluid of Cats

Griauzde¹,

Chen²,

Radulovački³

1979

Pharmacology

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The effects of diazepam (DZ) (0.3–1.5 mg/kg, i.p.) on sleep, cisternal cerebrospinal fluid (CSF) concentrations of 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA), and rectal temperature of cats were examined. The results showed that administration of DZ produced a significant increase (p = 0.02) in slow-wave sleep (SWS) with a peak occurring at a dose of 0.9 mg/kg. Further increase in doses of DZ decreased SWS. DZ administration produced no change in paradoxical sleep, rectal temperature, 5-HIAA or HVA CSF levels. Lack of correlation between various doses of DZ, and 5-HIAA or HVA concentrations in the presence of an increased percentage of SWS suggests a possible mode of DZ action mediated through a mechanism independent of monoamines. Usually monoamines are associated with normal sleep.

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Increase of 5‐hydroxyindoleacetic acid and homovanillic acid in cisternal fluid of cats subjected to stress

Cited by 14 publications

References 19 publications

Determination of Brain 5‐Hydroxytryptamine Turnover in Freely Moving Rats Using Repeated Sampling of Cerebrospinal Fluid

Determination of Brain 5‐Hydroxytryptamine Turnover in Freely Moving Rats Using Repeated Sampling of Cerebrospinal Fluid

Different neurotransmitter metabolite concentrations in CSF samples from inpatient and outpatient normal volunteers

Effects of Diazepam on Sleep, Temperature, 5-Hydroxyindoleacetic and Homovanillic Acids in Cisternal Cerebrospinal Fluid of Cats

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