Increase in the Solubility of Recombinant Mink Growth Hormone at Low Cultivation Temperature ofE. Coli

Optimization of Human Interferon α2b Soluble Protein Overproduction and Primary Recovery of Its Inclusion Bodies

“…Our result is comparable with previous work showing the overproduction of gluthatione S transferase hIFN2b fusion protein at 25 C with 0.5 mM IPTG and GFP CheY fusion protein at 25 C with 8 μM IPTG (Rabhi-Essafi 2007;Sevastsyanovich 2009). However, Cirkovas and Serekaite (2010) reported that overproduction conducted under low temperature 20 C improved the solubility of mink growth hormone. In this study, it was demonstrated that simultaneous decrease IPTG concentration into micromolar range did not reinforce the effect of temperature.…”

Growth hormone and growth hormone gene of the American mink (Neovison vison) – the current state of knowledge of one of the key hormones in one of the most intensively economically exploited species

“…It should be noted that a few studies on the mink somatotropin polypeptide are limited substantially to the development and improvement of methods for the obtaining the recombinant mGH in bacterial cells (Harada et al, 1994;Sereikaite et al, 2006;Sereikaite et al, 2007), as well as by optimizing the conditions of its storage and processing (Bajorunaite et al, 2007;Borromeo et al, 2008;Cirkovas, Sereikaite, 2010;Cirkovas, Sereikaite, 2011a,b;Zilinskas, Sereikaite 2011). This fact explains the scarcity of the available literature data on the specificity of the growth hormone molecule of N. vison (Sereikaite et al, 2006).…”