Increase in Phytoextraction Potential by Genome Editing and Transformation: A Review

Venegas-Rioseco, Javiera; Ginocchio, Rosanna; Ortiz-Calderón, Claudia

doi:10.3390/plants11010086

Cited by 24 publications

(12 citation statements)

References 129 publications

(153 reference statements)

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“…The widely used gene editing approaches such zinc finger nucleases (ZFNs) and transcript activators like effector nucleases (TALENs) is limited due to the frequent mutations at non-targeted sites (Sarma et al, 2021). The CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9) system has emerged as an innovative gene editing tool in plant systems (Venegas-Rioseco et al, 2021). It is a quick, cost friendly tool that can be used for improving crop traits against abiotic and biotic stress tolerance (Pandita, 2021).…”

Section: Genetic Engineering Approachesmentioning

confidence: 99%

“…The CRISPR–Cas9 (clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9) system has emerged as an innovative gene editing tool in plant systems ( Venegas-Rioseco et al., 2021 ). It is a quick, cost friendly tool that can be used for improving crop traits against abiotic and biotic stress tolerance ( Pandita, 2021 ).…”

Section: Various Approaches To Ameliorate Heavy Metal Stress In Medic...mentioning

confidence: 99%

“…It consists of spacer sequences placed between short palindromic repeats which transcribe to CRISPR RNA (crRNA), which combines with transactivating crRNA (tracrRNA) to form a mature crRNA/tracrRNA complex (guide RNA/gRNA). The gRNA directs the Cas nuclease, which creates a DNA double-strand break (DSB) in the desired DNA sequence, thereby causing gene deletion via the repair mechanisms of cells ( Rai et al., 2021 ; Venegas-Rioseco et al., 2021 ). Thus, gRNA-guided–Cas9 systems can be used for gene knockout, regulation of gene expression, and transcription factors, thereby enhancing heavy metal stress tolerance and phytoremediation in a diverse range of plants ( Bao et al., 2019 ).…”

Section: Various Approaches To Ameliorate Heavy Metal Stress In Medic...mentioning

confidence: 99%

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Cadmium toxicity in medicinal plants: An overview of the tolerance strategies, biotechnological and omics approaches to alleviate metal stress

et al. 2023

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Medicinal plants, an important source of herbal medicine, are gaining more demand with the growing human needs in recent times. However, these medicinal plants have been recognized as one of the possible sources of heavy metal toxicity in humans as these medicinal plants are exposed to cadmium-rich soil and water because of extensive industrial and agricultural operations. Cadmium (Cd) is an extremely hazardous metal that has a deleterious impact on plant development and productivity. These plants uptake Cd by symplastic, apoplastic, or via specialized transporters such as HMA, MTPs, NRAMP, ZIP, and ZRT-IRT-like proteins. Cd exerts its effect by producing reactive oxygen species (ROS) and interfere with a range of metabolic and physiological pathways. Studies have shown that it has detrimental effects on various plant growth stages like germination, vegetative and reproductive stages by analyzing the anatomical, morphological and biochemical changes (changes in photosynthetic machinery and membrane permeability). Also, plants respond to Cd toxicity by using various enzymatic and non-enzymatic antioxidant systems. Furthermore, the ROS generated due to the heavy metal stress alters the genes that are actively involved in signal transduction. Thus, the biosynthetic pathway of the important secondary metabolite is altered thereby affecting the synthesis of secondary metabolites either by enhancing or suppressing the metabolite production. The present review discusses the abundance of Cd and its incorporation, accumulation and translocation by plants, phytotoxic implications, and morphological, physiological, biochemical and molecular responses of medicinal plants to Cd toxicity. It explains the Cd detoxification mechanisms exhibited by the medicinal plants and further discusses the omics and biotechnological strategies such as genetic engineering and gene editing CRISPR- Cas 9 approach to ameliorate the Cd stress.

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Section: Genetic Engineering Approachesmentioning

confidence: 99%

Section: Various Approaches To Ameliorate Heavy Metal Stress In Medic...mentioning

confidence: 99%

Section: Various Approaches To Ameliorate Heavy Metal Stress In Medic...mentioning

confidence: 99%

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Cadmium toxicity in medicinal plants: An overview of the tolerance strategies, biotechnological and omics approaches to alleviate metal stress

et al. 2023

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“…Transgenic methods are not only used to convert functional genes but also to elevate particularly recognized promoters to existing gene functions connected with metal accumulation/translocation/detoxification mechanisms and introduce them to target bacteria ( Pratush et al, 2018 ). As a low molecular weight, cysteine-rich protein, metallothioneins (MTs) found in many bacteria have the ability to bind metals and form complex biochemical structures ( Venegas-Rioseco et al, 2022 ). Some gene transformation experiments have convincingly demonstrated that MT S produced by PGPB (e.g., P. putida and Mycobacterium tuberculosis ) can improve plant tolerance to metals ( Mierek-Adamska et al, 2017 ; Nanda et al, 2019 ).…”

Section: Genetically Modified Organisms For Bioremediationmentioning

confidence: 99%

Plant growth-promoting bacteria in metal-contaminated soil: Current perspectives on remediation mechanisms

et al. 2022

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Heavy metal contamination in soils endangers humans and the biosphere by reducing agricultural yield and negatively impacting ecosystem health. In recent decades, this issue has been addressed and partially remedied through the use of “green technology,” which employs metal-tolerant plants to clean up polluted soils. Furthermore, the global climate change enhances the negative effects of climatic stressors (particularly drought, salinity, and extreme temperatures), thus reducing the growth and metal accumulation capacity of remediating plants. Plant growth-promoting bacteria (PGPB) have been widely introduced into plants to improve agricultural productivity or the efficiency of phytoremediation of metal-contaminated soils via various mechanisms, including nitrogen fixation, phosphate solubilization, phytohormone production, and biological control. The use of metal-tolerant plants, as well as PGPB inoculants, should hasten the process of moving this technology from the laboratory to the field. Hence, it is critical to understand how PGPB ameliorate environmental stress and metal toxicity while also inducing plant tolerance, as well as the mechanisms involved in such actions. This review attempts to compile the scientific evidence on this topic, with a special emphasis on the mechanism of PGPB involved in the metal bioremediation process [plant growth promotion and metal detoxification/(im)mobilization/bioaccumulation/transformation/translocation] and deciphering combined stress (metal and climatic stresses) tolerance.

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“…These modifications facilitate to control and stabilize the harmful effects of environmental pollutants on various crop plants. CRISPR-Cas9-based gene editing offers exciting options for photo technologies such as phytoremediation [ 193 ]. Several phytoremediators have been sequenced such as Thlaspi caerulescens (hyper-accumulator for Ni, Zn, and Cd), Arabidopsis halleri (hyper-accumulator for Zn and Cd), Hirschfeldia incana (for controlling Pb), Brassica juncea , and Pteris vittata [ 194 , 195 , 196 , 197 ].…”

Section: Crispr For Improving Plant Tolerance To Climate Changementioning

confidence: 99%

Engineering Abiotic Stress Tolerance in Crop Plants through CRISPR Genome Editing

Rahman

Zulfiqar

Raza

et al. 2022

Cells

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Environmental abiotic stresses challenge food security by depressing crop yields often exceeding 50% of their annual production. Different methods, including conventional as well as genomic-assisted breeding, mutagenesis, and genetic engineering have been utilized to enhance stress resilience in several crop species. Plant breeding has been partly successful in developing crop varieties against abiotic stresses owning to the complex genetics of the traits as well as the narrow genetic base in the germplasm. Irrespective of the fact that genetic engineering can transfer gene(s) from any organism(s), transgenic crops have become controversial mainly due to the potential risk of transgene-outcrossing. Consequently, the cultivation of transgenic crops is banned in certain countries, particularly in European countries. In this scenario, the discovery of the CRISPR tool provides a platform for producing transgene-free genetically edited plants—similar to the mutagenized crops that are not extensively regulated such as genetically modified organisms (GMOs). Thus, the genome-edited plants without a transgene would likely go into the field without any restriction. Here, we focused on the deployment of CRISPR for the successful development of abiotic stress-tolerant crop plants for sustaining crop productivity under changing environments.

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Increase in Phytoextraction Potential by Genome Editing and Transformation: A Review

Cited by 24 publications

References 129 publications

Cadmium toxicity in medicinal plants: An overview of the tolerance strategies, biotechnological and omics approaches to alleviate metal stress

Cadmium toxicity in medicinal plants: An overview of the tolerance strategies, biotechnological and omics approaches to alleviate metal stress

Plant growth-promoting bacteria in metal-contaminated soil: Current perspectives on remediation mechanisms

Engineering Abiotic Stress Tolerance in Crop Plants through CRISPR Genome Editing

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