Increase in phenolic compounds of Coriandrum sativum L. after the application of a Bacillus halotolerans biofertilizer

Abstract

BACKGROUND There is an urgent need for a new sustainable way of satisfying the increasing demand for food worldwide. One of the main challenges is replacing chemical fertilizers with biofertilizers, which include plant root‐associated beneficial microorganisms. The present study reports, for the first time, the effects of SCCPVE07 bacterial strain with respect to improving not only plant development, but also the nutritional content and bioactive compounds content of Coriandrum sativum L., one of the most economically important crops, even for plant growth under salinity stress. RESULTS Innoculated coriander plants (C. sativum L.) showed an increase in potassium, carbon, calcium and iron content. A significant improvement in phenolic compounds contents was also observed. The contents of 5‐O‐caffeoylquinic acid, cinnamic acid, 4‐methoxy‐cinnamic acid hexoside, K‐3‐O rutinoside, Q‐3‐O‐rutinoside, Q‐3‐O‐glucoside and Q‐3‐O‐glucuronide were significantly enhanced. Moreover, an efficient bacterial root colonization and a noted growth promotion were demonstrated. Bacterial genome was sequenced and analysed. Gene coding related to Plant growth promotion (PGP) mechanisms and proteins involved in plant defence from salinity or in the metabolism of phenolic compounds, such as quercetin 2,3‐dioxygenase and phenolic acid decarboxylase, were identified. CONCLUSION The results obtained in the present study show, for the first time, the beneficial effects of the inoculation of a bacterial Bacillus halotolerans biofertilizer on coriander crops with respect to increasing the content in bioactive compounds and plant development. © 2020 Society of Chemical Industry

Rhizobium laguerreae Improves Productivity and Phenolic Compound Content of Lettuce (Lactuca sativa L.) under Saline Stress Conditions

“…The phenolic composition was determined as previously reported [20]. Briefly, plant samples were freeze-dried and, then, 5 mg were extracted using MeOH:H 2 O 80:20 (8 mL) in a bath of ultrasounds (30 min, three times).…”

“…Analyses by high performance liquid chromatography coupled to diode array detection and mass spectrometry (HPLC-DAD-MS) were performed to determine the phenolic composition of plant extracts. Samples were injected in a Hewlett-Packard 1200 series liquid chromatograph (Agilent Technologies, Waldbronn, Germany) using a Spherisorb ® S3 ODS-2 C18 reversed phase, 3 µm, 150 × 4.6 mm column (Waters Corporation, Milford, MA, USA) thermostatted at 35 • C. An aqueous solution of formic acid (1 mL L −1 ) (A) and acetonitrile (B) were used as solvents following the gradient previously reported [20]. Detection was carried out at 280, 330, and 370 nm as preferred wavelengths and spectra were recorded from 220 to 600 nm.…”

“…The development of genomic sequencing and mining has revolutionized the field of microbiology becoming a fundamental tool for the analysis and improvement of knowledge related to microbiological processes, such as microorganism-plant interactions [21]. Genome analysis allows the identification of genetic characteristics, as well as the relationships among its properties as bacterial biofertilizers [20]. The HUTR05 draft genome is composed by 156 contigs with a genome length of~7 Mbp, 7374 predicted coding sequences, and G + C content of 60.7%.…”

“…One of the alternatives that poses more potential to ensure productivity and sustainability within the field of agriculture is based on the use of plant growth promoting rhizobacteria (PGPR) capable of being used as biofertilizers, which, in turn, are naturally found in the soil. These bacteria favor crop yields by increasing the productivity and nutritional content of the plants with which they are associated, even in adverse environmental conditions [17][18][19][20].…”

Is the Application of Plant Probiotic Bacterial Consortia Always Beneficial for Plants? Exploring Synergies between Rhizobial and Non-Rhizobial Bacteria and Their Effects on Agro-Economically Valuable Crops

“…There are many reviews offering an overview of the vast information about microorganisms, and specially bacteria, that are plant growth promoters and biocontrol agents, and also reviewing the mechanisms associated with these particular functions, as well as more publications showing and/or reviewing the benefits to plants, such as the enhancement of bioactive compounds in the edible parts, among others [3,[12][13][14][15][76][77][78][79][80][81][82][83].…”