Increase in p21 expression independent of the p53 pathway in bleomycin-induced lung fibrosis

Abstract

Although a number of animal models have been used to study the pathogenesis of lung disease, to date few studies have looked at\ud changed in the expression of cell cycle regulatory genes. We have studied the variation in the expression of p21, p53, p27 and PCNA in\ud bleomycin-induced lung fibrosis using animal mouse models using immuno-histochemistry and gene-expression analysis. No difference in\ud the p53, PCNA and p27 expressions were observed from the bleomycin-induced fibrosis when compared to saline-induced non-fibrotic\ud lungs. Although no difference in nuclear p21 expression was observed, the level of cytoplasmic p21 expression was found to be higher in\ud fibrotic lungs at day 14 after bleomycin injection. p21 expression was found to increase independent of p53 in fibrotic lungs at 14 days after\ud bleomycin induction.peer-reviewe

A Novel, Non-Apoptotic Role for Scythe/BAT3: A Functional Switch between the Pro- and Anti-Proliferative Roles of p21 during the Cell Cycle

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Modelos experimentales para el estudio de la fibrosis pulmonar: utilidad práctica actual y futura

“…Cuando el gen normal se sustituye por otro alterado, con mutaciones específicas, recibe el nombre de knock-in. Actualmente es posible utilizar ratones con deleciones completas de un gen determinado para comprobar el efecto de la ausencia completa de su producto 26,[31][32][33][34][35] , estudiar los cambios en la expresión génica que originan diferentes inductores 36,37 , o bien provocar diferentes mutaciones genéticas para evaluar determinadas vías terapéuticas 38 . Para decidir qué genes estudiar, es útil investigar previamente cómo influye la inducción del proceso fibrótico en el cambio de la expresión genética.…”