Increase in microvascular permeability induced by enzymatically generated free radicals

Maestro, Rolando F. Del; Björk, Jakob; Arfors, Karl-E.

doi:10.1016/0026-2862(81)90095-9

Cited by 182 publications

(32 citation statements)

References 24 publications

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“…Del Maestro et al (Del Maestro et al, 1981a; Del Maestro et al, 1981b) employed its topical superfusion of tissue to examine changes in microvessel permeability, Parks et al (Parks et al, 1984) infused the mixture of HX/XO into the circulation to study the effects of oxygen radicals on intestinal vascular permeability, and Gaboury et al (Gaboury et al, 1993) employed infusions to elucidate the effects of NO on WBC-EC adhesion. The magnitude of WBC-EC adhesion (Fig.…”

Section: Discussionmentioning

confidence: 99%

The effect of doxycycline on shedding of the glycocalyx due to reactive oxygen species

Lipowsky

Lescanic

2013

Microvascular Research

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The structure and composition of the endothelial cell (EC) glycocalyx reflects a balance of the biosynthesis of glycans and their shear dependent removal. Shedding of glycans from the EC surface has been shown to occur in response to reactive oxygen species (ROS) and inflammatory mediators. Using sub-antimicrobial doses of doxycycline, a broad spectrum matrix metalloprotease (MMP) inhibitor, inhibition of chemoattractant induced glycan shedding has suggested that MMPs may be a major effector of the loss of glycans. However, it has also been reported that doxycycline is a scavenger of ROS that may also activate MMPs. To clarify the basis for doxycycline as an inhibitor of glycan shedding, the present studies were undertaken to determine its effect on ROS induced shedding in post-capillary venules of the exteriorized mesentery of the rat. To this end, hypoxanthine (HX) and xanthine oxidase (XO) were rapidly mixed on the mesenteric surface for a 2 min period to generate superoxide anion ( O2-•) and the time course of glycan shedding was monitored in post-capillary venules over a 30 min period. Glycan shedding was quantitated by loss of adherent fluorescently labeled lectin coated microspheres (FLMs, 0.1 μm diameter) that were systemically infused. It was found that HX/XO caused FLM adhesion to decrease 45% within 30 min. This effect could be inhibited in a dose dependent manner by the addition of superoxide dismutase to the superfusion solution, thus confirming the role of O2-•. In contrast, 0.5 μM doxycycline had no effect on FLM shedding in response to HX/XO, contrary to its ability to attenuate shedding in response to the chemoattractant fMLP. Thus it is suggested that the efficacy of doxycycline as an inhibitor of glycan shedding during inflammation arises from its ability to inhibit MMP activation.

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Section: Discussionmentioning

confidence: 99%

The effect of doxycycline on shedding of the glycocalyx due to reactive oxygen species

Lipowsky

Lescanic

2013

Microvascular Research

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“…In asthma, a variety of triggers including allergens and viruses, lead to the recruitment and activation of airway inflammatory cells, which produce excess ROS, resulting in oxidative damage. Oxidative damage causes many detrimental effects on airway function, including airway smooth muscle contraction [2], airway hyperresponsiveness [3], epithelial shedding [4] and vascular exudation [5,6]. Each of these effects contributes to the airway obstruction that is characteristic of asthma.…”

Section: Introductionmentioning

confidence: 99%

Airway and Circulating Levels of Carotenoids in Asthma and Healthy Controls

Wood

Garg

Blake

et al. 2005

Journal of the American College of Nutrition

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“…Albumin concentrations have been measured as an indicator of mucosal permeability in other experimental models. 19,35,36 In the study reported here, mucosal permeability was evaluated via albumin concentrations in the luminal perfusate. The albumin content in the lumen serves as an indicator of the extent to which the endothelial wall is compromised.…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of a customized solution for use in attenuating effects of ischemia and reperfusion in the equine small intestine

Hoogmoed¹,

Snyder²,

Nieto³

et al. 2001

ajvr

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Increase in microvascular permeability induced by enzymatically generated free radicals

Cited by 182 publications

References 24 publications

The effect of doxycycline on shedding of the glycocalyx due to reactive oxygen species

The effect of doxycycline on shedding of the glycocalyx due to reactive oxygen species

Airway and Circulating Levels of Carotenoids in Asthma and Healthy Controls

In vitro evaluation of a customized solution for use in attenuating effects of ischemia and reperfusion in the equine small intestine

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