Increase in dicentric chromosome formation after a single CT scan in adults

Abe, Yu; Miura, Tomisato; Yoshida, Mitsuaki A.; Ujiie, Risa; Kurosu, Yasuhiko; Kato, Nagisa; Katafuchi, Atsushi; Tsuyama, Naohiro; Ohba, Takayoshi; Inamasu, Tomoko; Shishido, Fumio; Noji, Hideyoshi; Ogawa, Kaoru; Yokouchi, Hiroshi; Kanazawa, Kenya; Ishida, Takashi; Muto, Satoshi; Ohsugi, Jun; Suzuki, Hiroyuki; Ishikawa, Tetsuo; Kamiya, Kenji; Sakai, Akira

doi:10.1038/srep13882

Cited by 39 publications

(36 citation statements)

References 25 publications

(37 reference statements)

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“…We recently showed that DIC formation increases significantly in PB lymphocytes after a single CT scan (5.78–60.27 mSv: mean 24.24 mSv) ( Supp. Table 1A and 1B ) [ 11 ]. We therefore recommended that 2000 or more metaphase figures in Giemsa-stained samples, and 1000 or more metaphase figures in Centromere-FISH be analyzed by DCAs in studies of low-dose radiation exposure [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…Table 1A and 1B ) [ 11 ]. We therefore recommended that 2000 or more metaphase figures in Giemsa-stained samples, and 1000 or more metaphase figures in Centromere-FISH be analyzed by DCAs in studies of low-dose radiation exposure [ 11 ]. DICs and chromosome translocations are produced in about an equal ratio in human PB cells after exposure to radiation [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Here we analyzed the frequency of chromosome translocations after a single CT scan: we used stored Carnoy's-fixed lymphocyte specimens taken from 12 patients in a previous study [ 11 ]. Based on our review of the published literature, there are no published studies comparing the frequency of chromosome translocations before and after a CT scan.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of chromosome translocation frequency after a single CT scan in adults

Abe¹,

Miura²,

Yoshida³

et al. 2016

Journal of Radiation Research

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We recently reported an increase in dicentric chromosome (DIC) formation after a single computed tomography (CT) scan (5.78–60.27 mSv: mean 24.24 mSv) and we recommended analysis of 2000 metaphase cells stained with Giemsa and centromere-FISH for dicentric chromosome assay (DCA) in cases of low-dose radiation exposure. In the present study, we analyzed the frequency of chromosome translocations using stored Carnoy's-fixed lymphocyte specimens from the previous study; these specimens were from 12 patients who were subject to chromosome painting of Chromosomes 1, 2 and 4. Chromosomes 1, 2 and 4 were analyzed in ∼5000 cells, which is equivalent to the whole-genome analysis of almost 2000 cells. The frequency of chromosome translocation was higher than the number of DICs formed, both before and after CT scanning. The frequency of chromosome translocations tended to be higher, but not significantly higher, in patients with a treatment history compared with patients without such a history. However, in contrast to the results for DIC formation, the frequency of translocations detected before and after the CT scan did not differ significantly. Therefore, analysis of chromosome translocation may not be a suitable assay for detecting chromosome aberrations in cases of low-dose radiation exposure from a CT scan. A significant increase in the frequency of chromosome translocations was not likely to be detected due to the high baseline before the CT scan; the high and variable frequency of translocations was probably due to multiple confounding factors in adults.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of chromosome translocation frequency after a single CT scan in adults

Abe¹,

Miura²,

Yoshida³

et al. 2016

Journal of Radiation Research

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“…Another important application of the method is in the refinement of dicentric chromosome assay (DCA), the gold standard for biodosimetry assessment of individuals after exposure to radiation [ 29 ]. The International Atomic Energy Agency recommends an analysis of more than 1000 cells for better ascertainment of dicentric chromosome formation for low radiation dose of less than 100 mSv, equivalent to a computed tomography (CT) scan [ 30 , 31 ]. A recent study by Abe et al [ 31 ] reported that the analysis of 1000 metaphase spreads probed with cenFISH was sufficient to yield comparable precision to 2000 conventional Giemsa stained metaphases.…”

Section: Resultsmentioning

confidence: 99%

“…The International Atomic Energy Agency recommends an analysis of more than 1000 cells for better ascertainment of dicentric chromosome formation for low radiation dose of less than 100 mSv, equivalent to a computed tomography (CT) scan [ 30 , 31 ]. A recent study by Abe et al [ 31 ] reported that the analysis of 1000 metaphase spreads probed with cenFISH was sufficient to yield comparable precision to 2000 conventional Giemsa stained metaphases. However, these DCA methods only assess for the occurrence of a dicentric chromosome based on the presence of primary constrictions and centromere DNA, therefore, not providing any information on whether the affected chromosome contains one or two active centromeres which may have impacts on downstream genome instability.…”

Section: Resultsmentioning

confidence: 99%

Active centromere and chromosome identification in fixed cell lines

2016

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BackgroundThe centromere plays a crucial role in ensuring the fidelity of chromosome segregation during cell divisions. However, in cancer and constitutional disorders, the presence of more than one active centromere on a chromosome may be a contributing factor to chromosome instability and could also have predictive value in disease progression, making the detection of properly functioning centromeres important. Thus far, antibodies that are widely used for functional centromere detection mainly work on freshly harvested cells whereas most cytogenetic samples are stored long-term in methanol-acetic acid fixative. Hence, we aimed to identify antibodies that would recognise active centromere antigens on methanol-acetic acid fixed cells.ResultsA panel of active centromere protein antibodies was tested and we found that a rabbit monoclonal antibody against human CENP-C recognises the active centromeres of cells fixed in methanol-acetic acid. We then tested and compared combinations of established methods namely centromere fluorescence in situ hybridisation (cenFISH), centromere protein immunofluorescence (CENP-IF) and multicolour FISH (mFISH), and showed the usefulness of CENP-IF together with cenFISH followed by mFISH (CENP-IF-cenFISH-mFISH) with the aforementioned anti-CENP-C antibody. We further demonstrated the utility of our method in two cancer cell lines with high proportion of centromere defects namely neocentromere and functional dicentric.ConclusionsWe propose the incorporation of the CENP-IF-cenFISH-mFISH method using a commercially available rabbit monoclonal anti-CENP-C into established methods such as dicentric chromosome assay (DCA), prenatal karyotype screening in addition to constitutional and cancer karyotyping. This method will provide a more accurate assessment of centromere abnormality status in chromosome instability disorders.

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Cytogenetic biodosimetry and dose-rate effect after radioiodine therapy for thyroid cancer

Saenko

Крылов

et al. 2017

Radiat Environ Biophys

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This study set out to investigate chromosomal damage in peripheral blood lymphocytes of thyroid cancer patients receiving I for thyroid remnant ablation or treatment of metastatic disease. The observed chromosomal damage was further converted to the estimates of whole-body dose to project the adverse side effects. Chromosomal aberration analysis was performed in 24 patients treated for the first time or after multiple courses. Blood samples were collected before treatment and 3 or 4 days after administration of 2-4 GBq of I. Both conventional cytogenetic and chromosome 2, 4 and 12 painting assays were used. To account for dose-rate effect, a dose-protraction factor was applied to calculate the whole-body dose. The mean dose was 0.62 Gy (95% CI: 0.44-0.77 Gy) in the subgroup of patients treated one time and 0.67 Gy (95% CI: 0.03-1.00 Gy) in re-treated patients. These dose estimates are about 1.7-fold higher than those disregarding the effect of exposure duration. In re-treated patients, the neglected dose-rate effect can result in underestimation of the cumulative whole-body dose by the factor ranging from 2.6 to 6.8. Elevated frequency of chromosomal aberrations observed in re-treated patients before radioiodine therapy allows estimation of a cumulative dose received from all previous treatments.

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Increase in dicentric chromosome formation after a single CT scan in adults

Cited by 39 publications

References 25 publications

Analysis of chromosome translocation frequency after a single CT scan in adults

Analysis of chromosome translocation frequency after a single CT scan in adults

Active centromere and chromosome identification in fixed cell lines

Cytogenetic biodosimetry and dose-rate effect after radioiodine therapy for thyroid cancer

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