Increase in cytotoxic lignans production after smut infection in sugar cane plants

Sánchez-Elordi, Elena; Sterling, Roberto M.; Santiago, Rocío; Armas, Roberto de; Vicente, Carlos; Legaz, Marı́a-Estrella

doi:10.1016/j.jplph.2019.153087

Cited by 9 publications

(5 citation statements)

References 50 publications

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“…Lignans constitute abundant classes of phenylpropanoids synthetized in many plants and they are related to plant defense systems. Structurally related lignan production is induced during plant–pathogen interaction [ 74 , 75 ], and we found the pal and chs genes, encoding the enzymes involved in the early steps of lignan biosynthesis [ 67 ], were upregulated during the P. patens fungal infection ( Figure 4 B). All lignan biosynthetic pathways initiate with deamination of phenylalanine by PAL [ 76 ].…”

Section: Resultsmentioning

confidence: 99%

Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Otero-Blanca

Pérez-Llano

Reboledo-Blanco

et al. 2021

JoF

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Anthracnose caused by the hemibiotroph fungus Colletotrichum gloeosporioides is a devastating plant disease with an extensive impact on plant productivity. The process of colonization and disease progression of C. gloeosporioides has been studied in a number of angiosperm crops. To better understand the evolution of the plant response to pathogens, the study of this complex interaction has been extended to bryophytes. The model moss Physcomitrium patens Hedw. B&S (former Physcomitrella patens) is sensitive to known bacterial and fungal phytopathogens, including C. gloeosporioides, which cause infection and cell death. P. patens responses to these microorganisms resemble that of the angiosperms. However, the molecular events during the interaction of P. patens and C. gloeosporioides have not been explored. In this work, we present a comprehensive approach using microscopy, phenomics and RNA-seq analysis to explore the defense response of P. patens to C. gloeosporioides. Microscopy analysis showed that appressoria are already formed at 24 h after inoculation (hai) and tissue colonization and cell death occur at 24 hai and is massive at 48 hai. Consequently, the phenomics analysis showed progressing browning of moss tissues and impaired photosynthesis from 24 to 48 hai. The transcriptomic analysis revealed that more than 1200 P. patens genes were differentially expressed in response to Colletotrichum infection. The analysis of differentially expressed gene function showed that the C. gloeosporioides infection led to a transcription reprogramming in P. patens that upregulated the genes related to pathogen recognition, secondary metabolism, cell wall reinforcement and regulation of gene expression. In accordance with the observed phenomics results, some photosynthesis and chloroplast-related genes were repressed, indicating that, under attack, P. patens changes its transcription from primary metabolism to defend itself from the pathogen.

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Section: Resultsmentioning

confidence: 99%

Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Otero-Blanca

Pérez-Llano

Reboledo-Blanco

et al. 2021

JoF

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“…36 Inoculation of sugarcane stems induces lignification, with a significantly greater increase in both lignins and lignan production observed in resistant cultivars compared to susceptible cultivars. 37,38 In this study, two PAL genes, ZB8 and PAL, were found to be up-regulated in both resistant and susceptible S. spontaneum during S. scitamineum infection, but the resistant line exhibited earlier and higher expression levels. The 4CL2/3/5 was also significantly up-regulated in resistant lines at 1, 3, and 5 dpi, while its expression remained constant in susceptible lines.…”

Section: ■ Discussionmentioning

confidence: 58%

A Comprehensive Analysis of Transcriptomics and Metabolomics Revealed Key Pathways Involved in Saccharum spontaneum Defense against Sporisorium scitamineum

Hu,

Luo,

et al. 2024

J. Agric. Food Chem.

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Sugarcane smut, caused by Sporisorium scitamineum, poses a severe threat to sugarcane production. The genetic basis of sugarcane resistance to S. scitamineum remains elusive. A comparative transcriptomic and metabolomic study was conducted on two wild Saccharum species of S. spontaneum with contrast smut resistance. Following infection, the resistant line exhibited greater down-regulation of genes and metabolites compared to the susceptible line, indicating distinct biological processes. Lignan and lignin biosynthesis and SA signal transduction were activated in the resistant line, while flavonoid biosynthesis and auxin signal transduction were enhanced in the susceptible line. TGA2.2 and ARF14 were identified as playing positive and negative roles, respectively, in plant defense. Exogenous auxin application significantly increased the susceptibility of S. spontaneum to S. scitaminum. This study established the significant switching of defense signaling pathways in contrast-resistant S. spontaneum following S. scitamineum infection, offering a hypothetical model and candidate genes for further research into sugarcane smut disease.

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“…The accumulation of sinapic acid is also consistent with systemic acquired resistance (Wang et al, 2020), since this compound is included among syringyl lignin monomers triggered by the hypersensitive response in plants. Finally, also the lignans accumulation we observed in organically grown tomatoes can be related to plant response to stress (Xiao et al, 2020) and secoisolariciresonl in particular to biotic stress (Sánchez-Elordi et al, 2020).…”

Section: Impact Of the Farming Systems (Organic Vs Conventional) On T...mentioning

confidence: 64%

The Hierarchical Contribution of Organic vs. Conventional Farming, Cultivar, and Terroir on Untargeted Metabolomics Phytochemical Profile and Functional Traits of Tomato Fruits

et al. 2022

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In this work, the impact of terroir, cultivar, seasonality, and farming systems on functional traits of tomato was hierarchically investigated. Untargeted metabolomics, antioxidant capacity, colorimetric assays, and enzyme inhibition were determined. The total phenolic and carotenoid contents significantly varied between growing years, whereas an interaction between the farming system and growing year (p < 0.01) was observed for total phenolics, carotenoids, and flavonoids, and for acetylcholinesterase inhibition. Hierarchical clustering showed that geographical origin and growing year were the major contributors to the differences in phytochemical profiles. Nonetheless, supervised modeling allowed highlighting the effect of the farming system. Several antioxidants (L-ascorbic acid, α-tocopherol, and 7,3′,4′-trihydroxyflavone) decreased, whereas the alkaloid emetine and phytoalexin phenolics increased under organic farming. Taken together, our findings indicate that cultivar and pedo-climatic conditions are the main determinants for the functional quality of tomato, whereas the farming system plays a detectable but hierarchically lower.

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Increase in cytotoxic lignans production after smut infection in sugar cane plants

Cited by 9 publications

References 50 publications

Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

A Comprehensive Analysis of Transcriptomics and Metabolomics Revealed Key Pathways Involved in Saccharum spontaneum Defense against Sporisorium scitamineum

The Hierarchical Contribution of Organic vs. Conventional Farming, Cultivar, and Terroir on Untargeted Metabolomics Phytochemical Profile and Functional Traits of Tomato Fruits

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