Increase in concentration of soluble HLA-G in high-quality embryos after intracytoplasmic sperm injection

Abstract

Non-invasive methods are normally preferred to conventional invasive methods when selecting suitable embryos to improve pregnancy rates after assisted reproduction techniques. One of the most recognized non-invasive methods is to examine the supernatants of embryo culture media. Soluble human leukocyte antigen, class I, G (sHLA-G) antigen is a non-classical class I molecule that has been widely considered as a marker of pregnancy failure or implantation success. In the current study of some Iranian patients, we examined the concentration of sHLA-G at different time points after intracytoplasmic sperm injection and compared the rates to the morphology and quality of the selected embryos. We showed that the concentration of sHLA-G increases over time in high-quality embryos. We conclude that there is a positive relationship between morphology, quality, and sHLA-G concentration. We suggest that this relationship can be used to increase the chance of a successful pregnancy.

Soluble human leukocyte antigen‐G is a potential embryo viability biomarker and a positive predictor of live‐births in humans

“…Consequently, they will have a huge bearing on the predictive value of sHLA-G as a biomarker for pregnancy progression and live-births. 49,56,71,80,81,87 In conclusion, we show that the embryo secreted sHLA-G could be a promising embryo viability biomarker, superior to embryo quality score, to predict prospective live-birth outcomes for improving eSET.…”

“…44,45 Embryo viability biomarkers are thought to be beneficial with a high predictive value for forecasting pregnancy outcome, which could act as an adjunct to embryo score. 33 The sHLA-G is one of the promising biomarkers considered in view of its following attributes: (1) sHLA-G expressed in pre-implantation embryos [46][47][48][49][50] and endometrium 51,52 involved in the immunoregulation of embryo development 25,26,53 and implantation, 54,55 (2) it positively correlates with embryo morphology, 30,31,55,56 IR [26][27][28]53,[57][58][59][60] and clinical pregnancy rates (CPR). 24,[27][28][29][30][31][32][33][34]53,57,59,60…”

The impact of soluble HLA-G in IVF/ICSI embryo culture medium on implantation success

“…In addition, the relationship between the quality of the embryo and the level of its HLAG secretion is still under discussion. Research by Desai et al (20), Rebmann et al ( 23) and Heidari et al (37) indicates that the best quality embryos secrete more sHLA-G than the inferior grade embryos (20, 23, 37). These results are in disagreement with the findings in other studies that the presence of sHLA-G in embryo cultures was not related to embryo quality determined morphologically (29,38,39).…”

Longitudinal profile of sHLA‐G during pregnancy and its association with small for gestational age births in North Indian pregnant females: A nested case‐control study

“…Aberrant levels of sHLA‐G protein in the peripheral circulation and in the placenta (mRNA/protein) of pregnant females has been linked to pregnancy complications, like preeclampsia, intrauterine growth restriction (IUGR) and adverse birth outcomes like recurrent pregnancy loss (RPL), and premature birth 11–15 . Moreover, low HLA‐G expression in human embryo culture supernatants was found to be correlated to their poor implantation in the in‐vitro fertilization process in humans 16,17 …”