Incorporation studies of nucleic acid precursors in gastric cancer

Schlag, Peter M.; Veser, J.; Geier, G; Breitig, D.; Merkle, P

doi:10.1007/bf00410648

Cited by 5 publications

(1 citation statement)

References 13 publications

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“…Information eoneerning the expected sensitivity or resistance of individual tumours to cytostatic agents before treatment would be extremely valuable and would enable the 80-90% of patients for whom this form of therapy is unlikely to be effeetive to be spared the likely adverse side effeets. Although sueh predietive studies have been reported to be successful with tumours from a variety of sites , Livingston et al 1980, see also Seetion 3:2:2), a reeent attempt to establish individualized chemotherapy for gastrie carcinoma patients has proved inconclusive (Schlag et al 1979).…”

Section: :4 Gastrointestinal Tractmentioning

confidence: 99%

Epithelial cell kinetics. A review of methods of study and their application to oral mucosa in health and disease

Scragg

Johnson

1982

J Oral Pathology Medicine

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In Part A, the techniques most commonly used to study epithelial cell kinetics and their suitability for use in man are reviewed. In Part B, the application of such methods to comparative studies of normal and abnormal oral epithelia of both man and experimental animals is examined. Claims regarding the possible prognostic value of LI's or MI's of biopsies of potentially or overtly malignant human oral lesions are also considered. Normal and abnormal kinetic data for other lining epithelia, e.g., skin, uterine cervix and gastrointestinal mucosa arc assessed where they provide a better illustration of some of the problems arising in such comparative investigations. Finally, the present and likely future areas in which cell kinetic studies may provide a guide to expected tumour prognosis and optimum treatment are summarized.

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Section: :4 Gastrointestinal Tractmentioning

confidence: 99%

Epithelial cell kinetics. A review of methods of study and their application to oral mucosa in health and disease

Scragg

Johnson

1982

J Oral Pathology Medicine

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Effect of fibroblast interferon on colony tumor growth of miscellaneous solid human tumors

et al. 1982

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The antiproliferative effect of fibroblast interferon (IF-beta) on the colony tumor growth of 42 miscellaneous carcinomas (15 gastric, 11 breast, eight colon, five ovarian, three bronchial) and two malignant melanomas were assayed in a semisolid, double-layered agar culture system. Of the 44 evaluable experiments only four tumor specimens (three breast-, one gastric carcinoma) showed at least a 30% inhibition of colony formation due to application of interferon at concentration of 1,000 IU/ml or less. In these cases, a dose response relationship of the interferon effect was obvious. Yet, in the majority of carcinomas investigated, interferon had no antiproliferative influence on colony tumor growth and was markedly inferior to a simultaneously tested cytostatic agent (adriamycin). Our in vitro results contradict a general direct cytotoxic action of interferon on human solid carcinomas.

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Postoperative active specific immunization in curatively resected colorectal cancer patients with a virus-modified autologous tumor cell vaccine

Lehner

Schlag

Liebrich

et al. 1990

Cancer Immunol Immunother

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Active specific immunotherapy was performed in a phase I study in 20 colorectal cancer patients after surgical resection of the tumor. An autologous tumor cell vaccine surface modified by Newcastle disease virus (NDV) was used, which showed the following characteristics. After mechanical and enzymatic dissociation of the tumor tissue an average of 5 x 10(7) cells/g tissue was obtained. According to trypan blue dye exclusion assay the average viability was 72%. Following irradiation (200 Gy) the inactivation of proliferative activity of the cells could be demonstrated by the absence of incorporation of 3H-labelled thymidine. The cells were, however, still metabolically active as shown by the incorporation of [3H]-uridine and a mixture of 3H-labelled amino acids. Epithelium-specific antigens (detected by mAb HEA125) were expressed on more than 75% cells of the cell suspension indicating a high amount of (epithelium-derived) tumor cells. In order to increase the immunogenicity of the tumor cells the suspended cells were infected by the nonlytic, apathogenic Ulster strain of NDV. The successful modification of tumor cells with NDV could be shown by electron microscopy. Three weeks postoperatively cells were thawed, virus-modified, and inoculated intradermally in the upper thigh. Several cell and virus concentrations were tested in each patient. As control, tumor cells without NDV, NDV alone and normal colon mucosa were used. The number of tumor cells ranged from 2 x 10(6) up to 2 x 10(7) cells and NDV concentrations from 4 to 64 hemagglutination units (HU) were tested. Sixteen patients responded with a delayed-type hypersensitivity (DTH) skin reaction to the vaccine. The best DTH reaction, measured 24 h following vaccination, was obtained using a vaccine consisting of 1 x 10(7) tumor cells and 32 HU NDV (median induration of 8 mm). Response to NDV alone was seen in 2 patients only (median induration of 3 mm); 12 patients responded to tumor cells (1 x 10(7) alone (median induration of 4 mm). Of 10 patients tested with normal colorectal mucosa, 4 responded with a median induration of 3.5 mm. DTH responses to the vaccine of 1 x 10(7) tumor cells and 32 HU NDV increased throughout the repeated vaccinations to a median induration of 9.5 mm at the end of the therapy. No severe side-effects in the course of the immunotherapy, except for mild fever in 4/20 patients, were observed. The results of our phase I study show that this type of autologous colorectal tumor cell vaccine is ready for a large clinical trial to prove its efficacy.

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Incorporation studies of nucleic acid precursors in gastric cancer

Cited by 5 publications

References 13 publications

Epithelial cell kinetics. A review of methods of study and their application to oral mucosa in health and disease

Epithelial cell kinetics. A review of methods of study and their application to oral mucosa in health and disease

Effect of fibroblast interferon on colony tumor growth of miscellaneous solid human tumors

Postoperative active specific immunization in curatively resected colorectal cancer patients with a virus-modified autologous tumor cell vaccine

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