Incorporation rates, stabilities, cytotoxicities and release of liposomal tetracycline and doxycycline in human serum [In Process Citation]

Sangaré, Lamba Omar; Morisset, R; Omri, Abdelwahab; Ravaoarinoro, M.

doi:10.1093/jac/42.6.831

Cited by 22 publications

(18 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the initial experiments, persistent cultures were exposed to doxycycline concentrations ranging from 0.05 to 50 g/ml. In these experiments, it was observed that exposure to doxcycline concentrations above 20 g/ml for 48 h was toxic to HeLa cells, consistent with previous observations with other mammalian cell lines (19,41). Doxycycline, at a concentration of 1 g/ml, was used alone or in combination with 0.2 mM L-1MT in the experiments reported here.…”

Section: Methodssupporting

confidence: 91%

Inhibition of Indoleamine 2,3-Dioxygenase Activity by Levo-1-Methyl Tryptophan Blocks Gamma Interferon-Induced Chlamydia trachomatis Persistence in Human Epithelial Cells

et al. 2011

View full text Add to dashboard Cite

show abstract

Section: Methodssupporting

confidence: 91%

Inhibition of Indoleamine 2,3-Dioxygenase Activity by Levo-1-Methyl Tryptophan Blocks Gamma Interferon-Induced Chlamydia trachomatis Persistence in Human Epithelial Cells

et al. 2011

View full text Add to dashboard Cite

show abstract

“…With the cell line HeLa 229 and the MGG staining method, the present study obtained low MIC values with liposomeencapsulated Tet and Dox which are close to glycylcycline anti-chlamydia1 activities [23]. Studies on the pharmacokinetics and in-vitro toxicity of these liposome-encapsulated drugs performed in this laboratory have shown that they have different patterns of stability in human serum but are not toxic for human erythrocytes and HeLa 229 cells in culture [24]. These results demonstrate that liposomal Tet and Dox could be very useful as an alternative strategy for antimicrobial therapy.…”

Section: Discussionsupporting

confidence: 52%

In-vitro anti-chlamydial activities of free and liposomal tetracycline and doxycycline

Sangaré

Morisset

Ravaoarinoro

1999

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to evaluate the anti-chlamydia1 activities in vitro of liposome-encapsulated doxycycline (Dox) and tetracycline (Tet) in comparison with free Dox and Tet. Dox and Tet encapsulated in cationic (CAL), anionic (ANL) and neutral (NTL) liposomes by sonication, were quantified by high-performance liquid chromatography. Anti-chlamydia1 activities were determined by addition of serial dilutions of antibiotics (MIC 0.12-0.007 mg/L; MBC 4-0.25 mg/L) to HeLa 229 cell monolayers inoculated with Chlamydia trachomatis L2 /434/Bu (lo3 ifu/well). After incubation for 72 h at 37"C, chlamydial inclusions were stained by the May-Griinwald Giemsa method to establish MICs. MBCs were determined in chlamydial agent-free medium after second passages. Dox-encapsulation efficiencies were 28.6 SEM 6.4% in cationic (CALDox), 49.1 SEM 6.7% in anionic (ANL-Dox) and 21.0 SEM 0.8% in neutral (NTL-Dox) liposomes. Tet-encapsulation efficiencies were 3.5 SEM 0.3% in anionic (ANL-Tet) and 2.2 SEM 0.6% in neutral (NTL-Tet) liposomes; no Tet was detected in cationic (CALTet) liposomes. MIC values were 0.06 mg/L for Dox, 0.12 mg/L for Tet, 0.03 mg/L for CAL-Dog, NTL-Dox and NTL-Tet, and 0.01 mg/L for ANL-Dox and ANL-Tet. MBCs were 4 mg/L for Tet, 0.5 mg/L for CAL-Dox and NTL-Dox, and 1 mg/L for Dox, ANLDox, ANL-Tet, NTL-Tet and NTL-Tet. For MICs, the relative increase in antichlamydial activity observed with liposomal formulations compared to the corresponding free antibiotic ranged from 2-to 6-fold with Dox and from 4-to 10-fold with Tet. For MBCs, the relative increases in anti-chlamydia1 activity were 2-and 4-fold with liposome-encapsulated Dox and Tet, respectively. Dox was better encapsulated than Tet in all liposomes. Liposome-encapsulated drugs showed greater anti-chlamydia1 activities than their free forms; thus, these drug formulations have potential in the treatment of chlamydial infections.

show abstract

“…At present, the most extensively investigated nonviral vector is cationic liposomes. They have been successfully used to deliver genes, proteins, oligonucleotides and antibiotics in several cell types [35,36,37]. They can provide slow release of encapsulated drugs, which lead to prolonged exposure to tumor cells and enhanced antitumor efficacy [38].…”

Section: Discussionmentioning

confidence: 99%

VEGF-Targeted Short Hairpin RNA Inhibits Intraperitoneal Ovarian Cancer Growth in Nude Mice

Bai¹,

Deng

Yang

et al. 2009

Oncology

View full text Add to dashboard Cite

Objective: In this study, we inhibited the expression of VEGF by short hairpin RNA (shRNA) in SKOV3 ovarian cancer cells in vitro and in vivo to explore the antitumor efficacy of shRNA in ovarian cancer cells. Methods: ShRNA targeting VEGF was cloned into pGenesil-2 plasmid vector and then transfected into SKOV3 ovarian cancer cells using liposome. Silencing of VEGF expression was measured by RT-PCR and ELISA assays. Furthermore, the growth inhibition capacity of shRNA on SKOV3 intraperitoneal ovarian carcinomatosis was tested in nude mice. Tumor weight was measured. Microvessel density, number of apoptotic cells and proliferation index in tumor tissues were assessed by CD31, TUNEL and PCNA immunostaining. Results: shRNA targeting VEGF significantly silenced VEGF expression in SKOV3 ovarian cancer cells, confirmed by RT-PCR and ELISA assays (p < 0.05). In vivo, the shRNA reduced tumor weight by ∼60.3% compared with control groups (p < 0.05), accompanied with angiogenesis inhibition (p < 0.01) and apoptosis induction (p < 0.01). Conclusion: Our data indicated that shRNA-mediated silencing of VEGF might be a promising therapeutic strategy against ovarian cancer by reducing angiogenesis and inducing apoptosis.

show abstract

Incorporation rates, stabilities, cytotoxicities and release of liposomal tetracycline and doxycycline in human serum [In Process Citation]

Cited by 22 publications

References 7 publications

Inhibition of Indoleamine 2,3-Dioxygenase Activity by Levo-1-Methyl Tryptophan Blocks Gamma Interferon-Induced Chlamydia trachomatis Persistence in Human Epithelial Cells

Inhibition of Indoleamine 2,3-Dioxygenase Activity by Levo-1-Methyl Tryptophan Blocks Gamma Interferon-Induced Chlamydia trachomatis Persistence in Human Epithelial Cells

In-vitro anti-chlamydial activities of free and liposomal tetracycline and doxycycline

VEGF-Targeted Short Hairpin RNA Inhibits Intraperitoneal Ovarian Cancer Growth in Nude Mice

Contact Info

Product

Resources

About