Incorporation of the novel bacterial blight resistance gene Xa38 into the genetic background of elite rice variety Improved Samba Mahsuri

Yugander, A.; Sundaram, R. M.; Singh, Kuldeep; Ladhalakshmi, D.; Rao, L. V. Subba; Madhav, M. S.; Badri, Jyothi; Prasad, M. S.; Laha, G. S.

doi:10.1371/journal.pone.0198260

Cited by 47 publications

(49 citation statements)

References 29 publications

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“…The analysis of variance showed highly significant value for all the traits undue study (Table 1). Kernel length of the introgression lines varied from 5.26 cm (ICF 2 -TH-625-588) to 6.46 cm (ICF 2 -TH-625-21), the kernel breadth varied (6), while the remaining lines had a slightly higher value (7). The KLAC of the lines were ranged from 8.43 to 9.97 mm but few lines were found to be better than Tellahamsa (9.43 mm).…”

Section: Agro-morphological and Quality Characters Of Selected Icf 2 mentioning

confidence: 96%

“…oryzae (Xoo) reduces rice yield drastically by declining photosynthetic area [6]. Disease survey data from the last 34 years within rice cultivating agroclimatic regions of India indicated that BB geographically spread rapidly, as a result of which, in recent years the extent of yield loss due to BB is more than 50% [7]. None of the several tested chemicals or antibiotics could control the BB infestation completely [8].…”

Section: Introductionmentioning

confidence: 99%

“…So far, more than 42 BB resistance genes have been identified; out of which 9 BB genes have been cloned [9]. In Indian environmental conditions, the combination of Xa21+ xa13 has been known to be more effective against the most virulent races existing across different agroclimatic zones [7].…”

Section: Introductionmentioning

confidence: 99%

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Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

et al. 2020

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Bacterial blight (BB) and fungal blast diseases are the major biotic constraints that limit rice productivity. To sustain yield improvement in rice, it is necessary to developed yield potential of the rice varieties by incorporation of biotic stress resistance genes. Tellahamsa is a welladapted popular high yielding rice variety in Telangana state, India. However, the variety is highly susceptible to BB and blast. In this study, simultaneous stepwise transfer of genes through marker-assisted backcross breeding (MABB) strategy was used to introgress two major BB (Xa21 and xa13) and two major blast resistance genes (Pi54 and Pi1) into Tellahamsa. In each generation (from F 1 to ICF 3) foreground selection was done using gene-specific markers viz., pTA248 (Xa21), xa13prom (xa13), Pi54MAS (Pi54) and RM224 (Pi1). Two independent BC 2 F 1 lines of Tellahamsa/ISM (Cross-I) and Tellahamsa/NLR145 (Cross-II) possessing 92% and 94% recurrent parent genome (RPG) respectively were intercrossed to develop ICF1-ICF 3 generations. These gene pyramided lines were evaluated for key agro-morphological traits, quality, and resistance against blast at three different hotspot locations as well as BB at two locations. Two ICF 3 gene pyramided lines viz., TH-625-159 and TH-625-491 possessing four genes exhibited a high level of resistance to BB and blast. In the future, these improved Tellahamsa lines could be developed as mega varieties for different agro-climatic zones and also as potential donors for different pre-breeding rice research.

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Section: Agro-morphological and Quality Characters Of Selected Icf 2 mentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

et al. 2020

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“…Breeding BB resistant rice varieties and hybrids is the best strategy for managing the BB disease in rice [ 10 ]. To date, at least 41 BB resistance genes have been identified,and some of them viz ., Xa4 , xa5 , xa13 , Xa21 have been extensively used for development of BB resistant rice varieties [ 11 , 12 , 13 , 14 , 15 ] ( Table 1 ). However, large scale and long-term cultivation of varieties and hybrids with a single gene results in the breakdown of resistance due to a high degree of pathogenic variation [ 12 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Marker-assisted pyramiding of two major, broad-spectrum bacterial blight resistance genes, Xa21 and Xa33 into an elite maintainer line of rice, DRR17B

Balachiranjeevi

Kumar

et al. 2018

PLoS ONE

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Bacterial blight (BB) disease reduces the yield of rice varieties and hybrids considerably in many tropical rice growing countries like India. The present study highlights the development of durable BB resistance into the background of an elite maintainer of rice, DRR17B, by incorporating two major dominant genes, Xa21 and Xa33 through marker-assisted backcross breeding (MABB). Through two sets of backcrosses, the two BB resistance genes were transferred separately to DRR17B. In this process, at each stage of backcrossing, foreground selection was carried out for the target resistance genes and for non-fertility restorer alleles concerning the major fertility restorer genes Rf3 and Rf4, using gene-specific PCR-based markers, while background selection was done using a set of 61 and 64 parental polymorphic SSR markers respectively. Backcross derived lines possessing either Xa21 or Xa33 along with maximum genome recovery of DRR17B were identified at BC3F1 generation and selfed to develop BC3F2s. Plants harboring Xa21 or Xa33 in homozygous condition were identified among BC3F2s and were intercrossed with each other to combine both the genes. The intercross F1 plants (ICF1) were selfed and the intercross F2(ICF2) plants possessing both Xa21 and Xa33 in homozygous condition were identified with the help of markers. They were then advanced further by selfing until ICF4 generation. Selected ICF4 lines were evaluated for their resistance against BB with eight virulent isolates and for key agro-morphological traits. Six promising two-gene pyramiding lines of DRR17B with high level of BB resistance and agro-morphological attributes similar or superior to DRR17B with complete maintenance ability have been identified. These lines with elevated level of durable resistance may be handy tool for BB resistance breeding.

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“…O. glaberrima accessions were screened from 2016 to 2019 using a local virulent isolate of Xoo viz., IX-020 (Yugander et al, 2018). The test entries along with recurrent parent and resistant check were raised in mud pots following the required care and fertilizer applications to raise healthy plants.…”

Section: Screening Of O Glaberrima For Bacterial Blight Resistancementioning

confidence: 99%

Identification and introgression of novel bacterial blight resistance from Oryza glaberrima Steud

Ajitha¹,

Laha²,

Sundaram³

et al. 2020

Preprint

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Background: Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the major diseases affecting rice production in India and globally. Developing resistant cultivar is generally regarded as the most effective and economical means of controlling this dreaded disease.Results: A total of 31 accessions of O. glaberrima were screened for BB resistance consecutively for four seasons and 28 accessions consistently exhibited high level of resistance with a mean lesion length of < 3cm. Molecular characterization using gene specific markers revealed absence of major resistance genes viz., xa5, xa13, Xa21, Xa38, xa41(t) and xa45 in these accessions, indicating the possibility of presence of novel gene(s) conferring BB resistance. In order to transfer BB resistance from O. glaberrima, resistant accessions EC861812 showing high level of BB resistance was crossed to the susceptible rice cultivar, IR64 and resultant interspecific hybrid plants were further backcrossed to IR64. Backcrossing was done up to BC2 generation and during each generation of backcrossing, the plants were evaluated for their BB reaction to identify BB resistant ones. High level of spikelet sterility was observed in F1 and subsequent backcross generations. BB reactions pattern in F1 and BC1F2 indicated single dominant gene conferring BB resistance in EC861812. We identified six resistant plants at BC2F2 (with mean lesion length of 0.1-0.8 cm) which were genotyped with 70 parental polymorphic SSR markers. The recovery of recurrent parent genome among the six resistant lines was found to be 65.1 to 84.5% with average of 67.5%. Interestingly, analysis of BC2F2 population for yield attributing traits showed transgressive segregation for plant height, productive tillers, panicle length which indicates that O. glaberrima also harbor beneficial alleles for yield related traits. Conclusion: BB resistant introgression lines derived from O. glaberrima identified in the present study will serve as precious pre-breeding resources for development of durable BB resistant elite breeding varieties.

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Incorporation of the novel bacterial blight resistance gene Xa38 into the genetic background of elite rice variety Improved Samba Mahsuri

Cited by 47 publications

References 29 publications

Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

Marker-assisted pyramiding of two major, broad-spectrum bacterial blight resistance genes, Xa21 and Xa33 into an elite maintainer line of rice, DRR17B

Identification and introgression of novel bacterial blight resistance from Oryza glaberrima Steud

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