Incorporation of Tetracycline in Phytohaemagglutinin Blast Cells

Lindgren, Ingvar

doi:10.1111/apm.1965.65.4.528

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…In most cases, lymphocytes can be indueed to undergo blast transformation and mitosis by adding a nonspecific stimulant, phytohemagglutinin (PHA). 194 One can add drugs to such a mixture in order to test effect on cell division or on reactivity to PHA.40, 130, 157,191,192,195,196,209,216,225,257,285 Another potential application would be to use drugs as anti genie stimulants in place of the PHA.240 This maneuver was successfully employed by Holland and Mauer,125 who studied the effects of hydantoins on a culture of lymphocytes from a girl with hydantoin sensitivity; under tissue culture conditions, the lymphocytes exposed to hydantoins enlarged, converted to blast forms, synthesized DNA, and engaged in mitotic division. Pencillin has similarly been found to induce blast transformation in sensitized patients' lymphocytes.…”

Section: Immunologie Drug Reactionsmentioning

confidence: 99%

Drug‐induced leukopenia and aplastic anemia

Pisciotta

1971

Clin Pharma and Therapeutics

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Hematopoietic damage following treatment with certain medications remains a major problem of drug therapy. As long as drugs remain indispensable in the treatment of disease, this problem cannot be resolved simply by avoiding their use. Instead, development of information that pertains to the mechanism and prediction of drug‐induced blood dyscrasias must be acquired for the safe use of drugs. Forewarned is forearmed; if it were possible to identify a susceptible patient or a potentially dangerous drug, then the potential risk of producing a serious blood re action with treatment would be minimized. A precedent for this concept was established with the discovery that patients whose erythrocytes are deficient in glucose‐6‐phosphate dehydrogenase are unusually susceptible to hemolytic anemia if treated with oxidant drugs.41 For most of the other toxic blood dyscrasias, knowledge of the pathogenesis remains obscure because certain difficulties limit acquisition of such information. The mechanisms of a drug reaction produced by one drug may differ from that produced by another drug. Also, the rare incidence of druginduced blood dyscrasias has made very few patients available for studies. Few papers have appeared which deal with the mechanism of drug‐induced hematopoietic disease. In addition, the difficulties entailed in obtaining bone marrow and other specimens in sufficient quantity has restricted effective investigation. Since the technique required to study mechanisms of drug reactions may be complex and expensive, it may be logistically unsound for a laboratory to “tool up” and then sit back and wait for the first patient to arrive.

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