Incorporation of Sodium Sulfite into Extraction Protocol Minimizes Degradation of
            <i>Acacia</i>
            DNA

Byrne, Margaret; MacDonald, B.; Francki, Michael G.

doi:10.2144/01304bm06

Cited by 59 publications

(49 citation statements)

References 8 publications

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“…Extraction of DNA from the leaves was carried out as in Byrne et al (1998) with 0.1 M sodium sulphite added to the extraction buffer (Byrne et al 2001). Seed was collected from each tree that produced seed in the small populations and 11-12 trees in the larger populations (33 seed crops in total).…”

Section: Plant Materials and Genotype Analysismentioning

confidence: 99%

Maintenance of high pollen dispersal in Eucalyptus wandoo, a dominant tree of the fragmented agricultural region in Western Australia

Byrne¹,

Elliott²,

Yates³

et al. 2007

Conserv Genet

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Pollen dispersal was investigated in five remnant populations of Eucalyptus wandoo, a dominant insect-pollinated tree in the fragmented agricultural region of southern Western Australia. Paternity analysis using six microsatellite loci identified a pollen source for 45% of seedlings, and the remainder were assumed to have arisen from pollen sources outside the stands. Outcrossing was variable, ranging from 52 to 89%, and long distance pollen dispersal was observed in all populations with up to 65% of pollen sourced from outside the populations over distances of at least 1 km. Modelling dispersal functions for pollination events within the two larger populations showed little difference between the four two-parameter models tested and indicated a fat-tailed dispersal curve. Similarity of direct and indirect historical estimates of gene flow indicates maintenance of gene flow at levels experienced prior to fragmentation. The study revealed extensive long distance pollen dispersal in remnant patches of trees within a fragmented agricultural landscape in the southern temperate region and highlighted the role of remnant patches in maintaining genetic connectivity at the landscape scale.

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Section: Plant Materials and Genotype Analysismentioning

confidence: 99%

Maintenance of high pollen dispersal in Eucalyptus wandoo, a dominant tree of the fragmented agricultural region in Western Australia

Byrne¹,

Elliott²,

Yates³

et al. 2007

Conserv Genet

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“…Leaf samples were also collected from 10 individuals in each of two populations of S. acuminatum as outgroups (Figure 1). DNA was extracted from the leaves of the 250 individuals as in Byrne and Moran (1994), with the addition of 0.1 M sodium sulphite to the extraction buffers (Byrne et al, 2001a). DNA quality was good, but the yield was low, probably due to high levels of sesquiterpene oils.…”

Section: Plant Collectionsmentioning

confidence: 99%

Phylogeography and divergence in the chloroplast genome of Western Australian Sandalwood (Santalum spicatum)

Byrne¹,

MacDonald²,

Brand³

2003

Heredity

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Western Australian sandalwood (Santalum spicatum) is widespread throughout Western Australia across the semiarid and arid regions. The diversity and phylogeographic patterns within the chloroplast genome of S. spicatum were investigated using restriction fragment length polymorphism analysis of 23 populations. The chloroplast diversity was structured into two main clades that were geographically separated, one centred in the southern (semiarid region) and the other in the northern (arid) region. Fragmentation due to climatic instability was identified as the most likely influence on the differentiation of the lineages. The lineage in the arid region showed a greater level of differentiation than that in the southern region, suggesting a higher level of gene flow or a more recent range expansion of sandalwood in the southern region. The phylogeographic pattern in the chloroplast genome is congruent with that detected in the nuclear genome, which identified different genetic influences between the regions and also suggested a more recent expansion of sandalwood in the southern region.

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“…The addition of sodium sulfi te to Tris-EDTA (extraction buffer) could inhibit the effect of phenols and tannins on DNA, hence good-quality DNA was obtained, which in turn improved the amplifi cation of DNA in PCR. In order to prevent interference of the polyphenolics in nucleic acid extraction the addition of sodium sulfi te to the extraction buffer has reduced the degradation of DNA and improved the yields, quality and stability of DNA (Byrne et al, 2001). Sodium sulfi te-extracted DNA survived at various temperatures much longer than that extracted without addition of sodium sulfi te (Baranwal et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

Detection of Huanglongbing (Citrus Greening) Disease by Nucleic Acid Spot Hybridization

Gopal

Sudarsan²,

Gopi

et al. 2009

Zeitschrift Für Naturforschung C

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Polymerase chain reaction (PCR) amplifi cation with primers specifi c to the rDNA region successfully amplifi ed the 1160-bp DNA fragment from a Huanglongbing (HLB)-infected sweet orange sample with mottling symptoms leaves, but not from healthy sweet orange plants. The PCR product of 1160-bp was used as probe labeled with biotin for detection of the HLB pathogen in the nucleic acid spot hybridization (NASH) test. It was found that the HLB pathogen could be detected up to 1:100 dilution in HLB-infected tissue. Total DNA extracted from HLB-infected tissue was diluted 2-fold as 900 ng in TE buffer and spotted on a nitrocellulose membrane. Strong signals were observed up to 225 ng of DNA dilution, whereas a moderate signal was recorded at 112 ng. No hybridization signal was observed in the healthy samples, while strong signals were observed in the positive control.

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Incorporation of Sodium Sulfite into Extraction Protocol Minimizes Degradation of Acacia DNA

Cited by 59 publications

References 8 publications

Maintenance of high pollen dispersal in Eucalyptus wandoo, a dominant tree of the fragmented agricultural region in Western Australia

Maintenance of high pollen dispersal in Eucalyptus wandoo, a dominant tree of the fragmented agricultural region in Western Australia

Phylogeography and divergence in the chloroplast genome of Western Australian Sandalwood (Santalum spicatum)

Detection of Huanglongbing (Citrus Greening) Disease by Nucleic Acid Spot Hybridization

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