Incorporation of silver nanoparticles on Ti7.5Mo alloy surface containing TiO2 nanotubes arrays for promoting antibacterial coating – In vitro and in vivo study

Abstract

Bulk and surface properties are very important for materials used in biomedical applications. The development of new surface treatments, such as antibacterial coatings can directly affect the response of the surface. The purpose of this study was the development of antibacterial coating on the Ti7.5Mo alloy surface combining TiO 2 nanotubes with silver nanoparticles incorporation using polydopamine assisted immobilization technique. Surface characterization analysis showed that silver nanoparticles were successfully immobilized. The concentration established was bactericidal, i.e., no bacteria grew after incubation. In vivo results showed that silver incorporation on the surface containing TiO 2 nanotubes did not cause altered locomotor activity in zebrafish. On the other hand, it affected the cell adhesion on the surface. Thus, these results confirm the hypothesis that silver nanoparticles were totally incorporated to polydopamine on the surface containing TiO 2 nanotubes and acted as the main mechanism the death of the bacteria by contact. The antibacterial activity of the coating was investigated on Escherichia

Obtaining of Antibacterial Nanoporous Layer on Ti7.5Mo Alloy Surface Combining Alkaline Treatment and Silver: In Vitro Studies

“…Surface modification techniques associated with the immobilization of bactericidal metallic agents can serve as an alternative for functionalizing these materials. Several methods have been developed, including chemical methods such as alkaline treatment [8][9][10], anodic oxidation [11][12][13], chemical vapor deposition [14,15], and sol-gel [16,17]. Alkaline treatment is a surface modification method that utilizes an NaOH solution at various concentrations and has been extensively studied.…”

“…In previous studies conducted by our group [11,12,20,21], we utilized two different types of surface treatment techniques, namely alkaline treatment and anodic oxidation, on the Ti7.5Mo alloy. The anodic oxidation process occurs in an electrochemical cell, consisting of two electrodes: a titanium anode and a platinum cathode.…”

“…The anodic oxidation process occurs in an electrochemical cell, consisting of two electrodes: a titanium anode and a platinum cathode. When a constant current or voltage is applied between the anode and the cathode, the oxidation and reduction reactions, in combination with the diffusion of ions in the electrolyte, lead to the formation of a porous TiO 2 layer on the surface of the anode [11,12]. In alkaline treatment with sodium hydroxide, the TiO 2 layer dissolves in the alkaline solution because of attacks by hydroxyl groups.…”

Evaluation of chlorhexidine /polydopamine antimicrobial coatings on the Ti-7.5 Mo alloy surface - in vitro studies

“…Several studies related to the development of antibacterial coatings on metal surfaces have already been performed and have effectively eliminated the infection and biofilm formation [6,7]. Among the antibacterial agents studied are chitosan, silver, and chlorhexidine [8,9,10,11,12]. Chlorhexidine is an antiseptic compound with high antibacterial action, with an effective action against a wide variety of gram-positive and gram-negative microorganisms, besides inhibiting the action of some fungi and enzymes [13].…”

Study of the capillary blood flow of the mucous membrane of the peri-implant area by laser Doppler flowmetry

“…Щодо поверхні імплантату показали: біоактивні покриття мають не тільки сприяти проліферації остеобластів і секреції кісткового матриксу, але й мінімізувати колонізацію бактерій на інженерній поверхні [23][24][25][26][27].…”