Incorporation of S35 in Healing Wounds in Normal and Devitalized Corneas

Dunnington, John H.; Smelser, George K.

doi:10.1001/archopht.1958.00940080130016

Cited by 53 publications

(7 citation statements)

References 8 publications

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“…12 c to g); however, this was not observed in one case where the amount of S 35 still remained comparable in degree to that of the host cornea. Grafts allowed to become partially opaque and vascularized showed a decreased amount of S 8s in the hazy, non-infiltrated area; but in the part of the graft where invasion by vessels and fibroblasts was present, increased uptake of sulfate was observed, as would be expected (29) in a rapidly growing scar tissue (Fig. 12 h).…”

Section: Alterations In the Metachromaticmentioning

confidence: 55%

Histopathological and Histochemical Alterations in the Early Stages of Corneal Graft Rejection

Polack

1962

The Journal of Experimental Medicine

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Rejection of corneal grafts was produced in rabbits after skin from the corneal donor was grafted subcutaneously. Clinical observations showed that the graft sickness started at the periphery of the graft after blood vessels from the host cornea reached the scar. Histologic studies demonstrated that the scar tissue was first invaded by vessels and infiltrated by lymphocytes, monocytes, and plasma cells. This infiltrate reached the endothelium through the gap in Descemet's membrane. The early histological picture of the graft sickness was characterized by endothelial destruction and infiltration at its junction with the scar. Keratocytes changed in shape and apparently became active fibroblasts. Decreased amounts of ground substance in the early stages of rejection, as indicated by appearance of haziness in the graft, were indicated in sections by a decrease in metachromatic staining. Diminished uptake of radioactive sulfate also occurred in the early stages of the graft sickness; which was followed by an elevated uptake of sulfate as the graft became opaque and filled with active fibroblasts.

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Section: Alterations In the Metachromaticmentioning

confidence: 55%

Histopathological and Histochemical Alterations in the Early Stages of Corneal Graft Rejection

Polack

1962

The Journal of Experimental Medicine

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“…Computational studies also have proposed that GAGs bridge and transfer force between adjacent fibrils, thus improving the mechanical integrity of the tissue (Redaelli et al, 2003). Interestingly, a correlation has been detailed between the appearance of metachromatic glycosaminoglycan staining in stromal ECM and the appearance of the highly organized collagen fibril packing during the acquisition of corneal transparency in the developing embryo(Coulombre and Coulmbre, 1958; Coulombre and Coulombre, 1958) In contrast, there is a loss of such metachromatic staining in opaque corneal wounds, which coincides with a reduction in the levels of corneal keratan sulfate (Anseth, 1961; Dunnington and Smelser, 1958). Consequently, the GAG chains on SLRPs can not only be involved in the regulation of fibrillogenesis, but can also influence inter-fibril spacing as well as organization during matrix assembly (Fig.…”

Section: Regulation Of Corneal Stroma Extracellular Matrix Assemblymentioning

confidence: 99%

Regulation of corneal stroma extracellular matrix assembly

Chen

Mienaltowski

Birk

2015

Experimental Eye Research

134

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The transparent cornea is the major refractive element of the eye. A finely controlled assembly of the stromal extracellular matrix is critical to corneal function, as well as in establishing the appropriate mechanical stability required to maintain corneal shape and curvature. In the stroma, homogeneous, small diameter collagen fibrils, regularly packed with a highly ordered hierarchical organization, are essential for function. This review focuses on corneal stroma assembly and the regulation of collagen fibrillogenesis. Corneal collagen fibrillogenesis involves multiple molecules interacting in sequential steps, as well as interactions between keratocytes and stroma matrix components. The stroma has the highest collagen V:I ratio in the body. Collagen V regulates the nucleation of protofibril assembly, thus controlling the number of fibrils and assembly of smaller diameter fibrils in the stroma. The corneal stroma is also enriched in small leucine-rich proteoglycans (SLRPs) that cooperate in a temporal and spatial manner to regulate linear and lateral collagen fibril growth. In addition, the fibril-associated collagens (FACITs) such as collagen XII and collagen XIV have roles in the regulation of fibril packing and inter-lamellar interactions. A communicating keratocyte network contributes to the overall and long-range regulation of stromal extracellular matrix assembly, by creating micro-domains where the sequential steps in stromal matrix assembly are controlled. Keratocytes control the synthesis of extracellular matrix components, which interact with the keratocytes dynamically to coordinate the regulatory steps into a cohesive process. Mutations or deficiencies in stromal regulatory molecules result in altered interactions and deficiencies in both transparency and refraction, leading to corneal stroma pathobiology such as stromal dystrophies, cornea plana and keratoconus.

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“…There was a correlation between the appearance of metachromatic staining of the glycosaminoglycans in stromal ECM and appearance of the highly organized collagen lattice during the acquisition of corneal transparency in the developing embryo (Coulombre and Coulombre 1958). There was also a loss of the metachromatic staining in opaque corneal wounds (Dunnington and Smelser 1958) that coincided with a reduction in the levels of corneal keratan sulfate (Anseth 1961), a glycosaminoglycan determined later to be a component of some proteoglycans. These observations generated considerable interest in characterizing the collagens and proteoglycans in the corneal stroma and determining their roles in the formation of a transparent ECM.…”

Section: Introductionmentioning

confidence: 99%