Incorporation of PEG-lipids in arsonoliposomes results in formation of highly stable arsenic-containing vesicles

Piperoudi, Sophia; Fatouros, D.G.; Ioannou, Panayiotis V.; Frederik, Peter M.; Antimisiaris, Sophia G.

doi:10.1016/j.chemphyslip.2005.11.003

Cited by 30 publications

(31 citation statements)

References 30 publications

(41 reference statements)

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“…A similar stabilizing effect of PEGylation (performed by adding 8 mol% of DSPE-PEG2000 in the lipid phase during arsonoliposome preparation) was demonstrated previously in the case of DSPC-based arsonoliposomes (with the specific lipid composition which is used also in this study) [7]. Nevertheless, when the stability (in FCS) of the PEGylated PC arsonoliposomes studied herein, is compared to that of the PEGylated DSPC asronoliposomes studied before (under the same conditions) [7], it becomes obvious that PEGylated DSPC arsonoliposomes (the values for which were added in Fig. 3 (as triangles) for direct comparison) are more stable (calcein latency is 90 and 65% after 5 min and 24 h incubation, respectively) compared to the PC ones.…”

Section: Arsonoliposome Physicochemical Propertiessupporting

confidence: 67%

“…Recently it was noticed that arsonoliposomes are sensitive to glutathione and their integrity decreases when they are incubated in glutathione-rich solutions [12]. However, some arsonoliposome types with high integrity (as DSPC-based and PEGylated arsonoliposomes) [6,7] which were lately demonstrated to have better bioavailabity after in vivo injection (compared to arsonoliposomes with low integrity (PC- based)) [9], were not glutathione-sensitive [12]. In order to evaluate if the previously demonstrated anticancer activity of the more leaky PC-based arsonoliposomes [3,4] is not abolished in the case of more stable arsonoliposomes and to study the general effect of arsonoliposome lipid composition on their activity, we investigated herein the anticancer activity of various types of stable arsonoliposomes on different types of cancer cells, in culture.…”

Section: Discussionmentioning

confidence: 98%

“…As summarized before [2], promising results were obtained with some of the arsonoliposome types prepared, for which a differential toxicity towards cancer and normal cells was demonstrated [3,4] as well as in vitro antiparasitic activity [5]. However, it has been found that the membrane integrity of arsonoliposomes [6,7] and their in vivo distribution (of arsenic) [8,9] are influenced by the lipid composition of the arsonoliposome membrane, and this has been pinpointed as the reason -or at least one of the reasons -for the different antiparasitic activities demonstrated (in vitro and in vivo), by different arsonoliposome types (with different lipid compositions) [10].…”

Section: Introductionmentioning

confidence: 93%

“…Since PEGylated PC arsonoliposomes were prepared for the first time, the integrity of this type of arsonoliposomes was evaluated by measuring the release of vesicle-encapsulated calcein, during incubation of the vesicles in buffer or in presence of serum proteins (80% FCS), for 24 h, as studied before for PEGylated (and non-PEGylated) DSPC arsonoliposomes [7]. The integrity of non-PEGylated PC arsonoliposomes was also evaluated under identical conditions for comparison.…”

Section: Arsonoliposome Physicochemical Propertiesmentioning

confidence: 99%

“…Arsonolipid (Ars)-containing liposomes were prepared as described previously [1,2,6,7]. In brief, lipids (after removing the organic solvents with a nitrogen stream) were mixed with 5 mM phosphate buffer (pH 7.4) and 20 mM NaCl and magnetically stirred vigorously on a hot plate for 4 h at 70 -808C.…”

Section: Preparation Of Arsonoliposomesmentioning

confidence: 99%

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Does the lipid membrane composition of arsonoliposomes affect their anticancer activity? A cell culture study

Zagana

Haikou

Giannopoulou

et al. 2009

Molecular Nutrition Food Res

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Sonicated arsonoliposomes were prepared using arsonolipid with palmitic acid acyl chain (C16), mixed with phosphatidylcholine (PC)-based or 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)-based, and cholesterol (Chol) with C16/DSPC/Chol 8:12:10 molar ratio. PEG-lipid (1,2-distearoyl-sn-glycero-3-phosphoethanolamine conjugated to polyethylenoglycol 2000) containing vesicles (PEGylated-arsonoliposomes; PC-based and DSPC-based) were also prepared. The cytotoxicity of these arsonoliposomes towards different cancer cells (human promyelocytic leukaemia NB4, Prostatic cancer PC3, human breast adenocarcinoma MDA-MB-468, human T-lymphocyte (MT-4) and also towards human umbilical vein endothelial cells (HUVECs) was evaluated by calculating the arsonoliposome-induced growth inhibition of the cells by the MTT assay. IC-50 values were interpolated from cell number/arsonoliposome concentration curves. The results reveal that all types of arsonoliposomes evaluated significantly inhibit the growth of most of the cancer cells studied (PC3, NB4, MT4) with the exception of the MDA-MB-468 breast cancer cells which were minimally affected by arsonoliposomes; in some cases even less than HUVEC. Nevertheless, for the same cell type the differences between the different types of arsonoliposomes were significant but not proportional to their stability, indicating that the formation of arsonoliposomes with very stable membranes is not a problem for their anticancer activity. Thereby it is concluded that arsonoliposome composition should be adjusted in accordance to their in vivo kinetics and the desired, for each specific application, biodistribution of As and/or encapsulated drug.

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Section: Arsonoliposome Physicochemical Propertiessupporting

confidence: 67%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 93%

Section: Arsonoliposome Physicochemical Propertiesmentioning

confidence: 99%

Section: Preparation Of Arsonoliposomesmentioning

confidence: 99%

See 3 more Smart Citations

Does the lipid membrane composition of arsonoliposomes affect their anticancer activity? A cell culture study

Zagana

Haikou

Giannopoulou

et al. 2009

Molecular Nutrition Food Res

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Aerosol-Stable Peptide-Coated Liposome Nanoparticles: A Proof-of-Concept Study with Opioid Fentanyl in Enhancing Analgesic Effects and Reducing Plasma Drug Exposure

Hoekman

Srivastava

2014

Journal of Pharmaceutical Sciences

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Previous we reported a novel pressurized olfactory drug (POD) delivery device that deposit aerosolized drug preferentially to upper nasal cavity. This POD device provided sustained CNS levels of soluble morphine analgesic effects. However, analgesic onset of less soluble fentanyl was more rapid but brief, likely due to hydrophobic fentanyl redistribution readily back to blood. To determine whether fentanyl incorporated into an aerosol stable liposome that binds to nasal epithelial cells will enhance CNS drug exposure and analgesic effects and reduce plasma exposure, we constructed RGD liposomes anchored with acylated integrin binding peptides (palmitoyl-GRGDS). The RGD liposomes, which assume gel-phase membrane structure at 25°C were stable under the stress of aerosolization as only 2.2 ± 0.5 % calcein leakage detected. The RGD mediated integrin binding of liposome is also verified to be unaffected by aerosolization. Rats treated with fentanyl in RGD-liposome and POD device exhibited greater analgesic effect, compared to the free drug counterpart (AUCeffect = 1387.l vs. 760.1 %MPE*min); while ~20% reduced plasma drug exposure was noted (AUC0-120 = 208.2 vs 284.8 ng*min/ml). Collectively, fentanyl incorporated in RGD-liposomes are physically and biologically stable under aerosolization, enhanced the overall analgesic effects and reduced plasma drug exposure for the first 2 hours.

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Organic Arsenicals as Functional Motifs in Polymer and Biomaterials Science

Tanaka

Davis

Wilson

2018

Macromol. Rapid Commun.

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Arsenic (As) exhibits diverse (bio)chemical reactivity and biological activity depending upon its oxidation state. However, this distinctive reactivity has been largely overlooked across many fields owing to concerns regarding the toxicity of arsenic. Recently, a clinical renaissance in the use of arsenicals, including organic arsenicals that are known to be less toxic than inorganic arsenicals, alludes to the possibility of broader acceptance and application in the field of polymer and biomaterials science. Here, current examples of polymeric/macromolecular arsenicals are reported to stimulate interest and highlight their potential as a novel platform for functional, responsive, and bioactive materials.

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Incorporation of PEG-lipids in arsonoliposomes results in formation of highly stable arsenic-containing vesicles

Cited by 30 publications

References 30 publications

Does the lipid membrane composition of arsonoliposomes affect their anticancer activity? A cell culture study

Does the lipid membrane composition of arsonoliposomes affect their anticancer activity? A cell culture study

Aerosol-Stable Peptide-Coated Liposome Nanoparticles: A Proof-of-Concept Study with Opioid Fentanyl in Enhancing Analgesic Effects and Reducing Plasma Drug Exposure

Organic Arsenicals as Functional Motifs in Polymer and Biomaterials Science

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