Incorporation of lycopene into chylomicron remnant-like particles inhibits their uptake by HepG2 cells

Bejta, Fatos; Napolitano, Mariarosaria; Botham, Kathleen M.; Bravo, Elena

doi:10.1016/j.lfs.2007.01.051

Cited by 2 publications

(2 citation statements)

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“…The liver plays an important role in the lipid metabolism. The human liver cell line HepG2 was previously used as a biological model to study the effect of phytochemicals and remnantlike chylomicrons enriched in bioactive compounds (13)(14)(15)(16); *To whom correspondence should be addressed. Telephone: þ34-868-884-942.…”

Section: Introductionmentioning

confidence: 99%

“…The liver plays an important role in the lipid metabolism. The human liver cell line HepG2 was previously used as a biological model to study the effect of phytochemicals and remnant-like chylomicrons enriched in bioactive compounds − ; nevertheless, they have not been incubated directly with the postprandial human chylomicron serum fraction. In HepG2 cells, it is easy to detect changes on the antioxidant status in response to the bioactive compounds of different vegetables , but this method does not reflect the physiological situation because liver cells are not in direct contact with vegetable products but with chylomicrons.…”

Section: Introductionmentioning

confidence: 99%

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Cell-Based Assay To Quantify the Antioxidant Effect of Food-Derived Carotenoids Enriched in Postprandial Human Chylomicrons

Sánchez-Campillo

Pérez‐Llamas

González-Silvera

et al. 2010

J. Agric. Food Chem.

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We developed a new method to evaluate the antioxidant effect of food products in a biological system. The antioxidant status of HepG2 cells was quantified after incubation with postprandial human chylomicrons after the intake of vegetable products. Three subjects consumed in a meal a vegetable soup containing 8.4 mg of β-carotene and 9 mg of lycopene. After 5 h, the subjects consumed a second meal without carotenoids. Blood samples were collected at basal time and every hour for 9 h. Chylomicrons were isolated from serum samples and used for both carotenoid quantification and HepG2 stimulation. Carotenoid in chylomicrons followed an inter-individual and bimodal carotenoid response. We demonstrated the antioxidant effect of postprandial chylomicrons in HepG2 at the time of maximum carotenoid concentration of chylomicrons with respect to basal time. This cell-based assay seems to be a useful method to evaluate the antioxidant effect of fruit and vegetable products in a biological system.

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“…The liver plays an important role in the lipid metabolism. The human liver cell line HepG2 was previously used as a biological model to study the effect of phytochemicals and remnantlike chylomicrons enriched in bioactive compounds (13)(14)(15)(16); *To whom correspondence should be addressed. Telephone: þ34-868-884-942.…”

Section: Introductionmentioning

confidence: 99%

“…The liver plays an important role in the lipid metabolism. The human liver cell line HepG2 was previously used as a biological model to study the effect of phytochemicals and remnant-like chylomicrons enriched in bioactive compounds − ; nevertheless, they have not been incubated directly with the postprandial human chylomicron serum fraction. In HepG2 cells, it is easy to detect changes on the antioxidant status in response to the bioactive compounds of different vegetables , but this method does not reflect the physiological situation because liver cells are not in direct contact with vegetable products but with chylomicrons.…”

Section: Introductionmentioning

confidence: 99%