Incorporation of <i>Boswellia sacra</i> essential oil into chitosan/TPP nanoparticles towards improved therapeutic efficiency

Soltani, Mohammad Reza; Etminan, Ayda; Rahmati, Amir; Moghadam, Mahsa Behjati; Segonbad, Ghazaleh Ghaderi; Tabrizi, Masoud Homayouni

doi:10.1080/10667857.2021.1976364

Cited by 23 publications

(9 citation statements)

References 70 publications

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“…Similar to our result, several studies have indicated that frankincense and its components enhanced apoptosis through ROS-dependent production [26], [47].…”

Section: Figure 5: (I) a Photomicrograph Of Scc-25 Cells 24 H After T...supporting

confidence: 92%

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An Ex vivo Apoptotic and Cytotoxic Effects of Frankincense on Oral Squamous Cell Carcinoma Cell Line

Mostafa

El-Bolok

Fahmi

et al. 2022

Open Access Maced J Med Sci

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BACKGROUND: Oral squamous cell carcinoma is accounting for almost 90% of oropharyngeal cancer diagnoses. Natural herbal medicine can use as an alternative, or complementary, or adjunctive for cancer treatment. Frankincense and its combination with myrrh have anticancer effects on different cancer types. METHODS: In this research, aqueous and methanolic extracts of frankincense and the combination of aqueous extract of frankincense and myrrh were applied on tongue squamous cell carcinoma cell line to study their cytotoxic and apoptotic effect by the assessment of cell viability and cytotoxicity, caspase 3 and 8 activation, reactive oxygen species activity, mitochondrial membrane potential, morphological changes, and nuclear area factor measurements. RESULTS: The result showed that aqueous and methanolic extracts of frankincense have cytotoxic and apoptotic effects in a concentration-dependent manner with an IC50 value of 21.05 ± 1.27 μM for aqueous extract, 36.72 ± 2.07 μM for methanolic extract, and IC50 value of 1.31 ± 0.04 μM for combination of aqueous extract of frankincense and myrrh extract after 24 h. CONCLUSION: Different extracts of frankincense and the combination of aqueous extract of frankincense and myrrh extract exhibited cytotoxic and apoptotic effects by reducing the cell viability and activating caspases 3 and 8 causing intrinsic- and extrinsic-mediated apoptosis pathways activation with the involvement of oxidative stress that was conceivable with cytonuclear morphological alterations results.

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“…Similar to our result, several studies have indicated that frankincense and its components enhanced apoptosis through ROS-dependent production [26], [47].…”

Section: Figure 5: (I) a Photomicrograph Of Scc-25 Cells 24 H After T...supporting

confidence: 92%

“…Frankincense and its isolated components induce apoptosis (intrinsic and extrinsic) in a dose-dependent way in different cancer cell lines [14], [45], [46], [47] through activation of caspases 3 and 8 [25], [26], [48], [49].…”

Section: Figure 5: (I) a Photomicrograph Of Scc-25 Cells 24 H After T...mentioning

confidence: 99%

An Ex vivo Apoptotic and Cytotoxic Effects of Frankincense on Oral Squamous Cell Carcinoma Cell Line

Mostafa

El-Bolok

Fahmi

et al. 2022

Open Access Maced J Med Sci

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“…The amount of free radical inhibition by ARC‐PCF‐NPs was also evaluated by performing the 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) test 14 . As previously reported, 500 μL of different concentrations of ARC‐PCF‐NPs were mixed with 500 μL of dissolved ABTS in potassium persulfate and deionized distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…The amount of free radical inhibition by ARC-PCF-NPs was also evaluated by performing the 2,2′-azino-bis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS) test. 14 As previously reported, 500 μL of different concentrations of ARC-PCF-NPs were mixed with 500 μL of dissolved ABTS in potassium persulfate and deionized distilled water. After 1 h of incubation, the absorbance of the samples was read at 734 nm, and the amount of free radical inhibition was calculated through the following formula 12 :…”

Section: Abts Assaymentioning

confidence: 99%

Evaluation of the effect of designed PLGA‐arctiin nanoparticles modified with folic acid and chitosan on colon cancer cells

Alhadidi,

Tabrizi,

Ghobeh

2023

Biotech and App Biochem

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In this study, we designed nanoparticles (NPs) based on polylactic acid glycolic acid modified with chitosan and folic acid to optimize the anti‐cancer, anti‐inflammatory, and antioxidant effects of arctiin (ARC), and we measured its effects on cancer cells, including colon cancer. NPs were synthesized using the W1/O/W2 double‐emulsion solvent evaporation method. Physicochemical characteristics of synthesized NPs (ARC‐PCF‐NPs), including average particle size, dispersity index (PDI), zeta potential (ZP), field emission scanning electron microscope figures, and encapsulation efficiency (EE), were evaluated. 2,2′‐Azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS), 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) methods were carried out to determine the antioxidant properties of NPs. 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H‐tetrazolium bromide assay was performed to investigate cytotoxicity effects on cancer cells and normal fibroblasts. Quantitative polymerase chain reaction was also performed on inflammatory and antioxidant genes. The obtained results indicated that the synthesized NPs have a size of 100 nm, a DPI of 0.36, a ZP of 26.30 mV, and EE was calculated at about 87.5%. The antioxidant influence of ARC‐PCF‐NPs was confirmed by inhibiting ABTS and DPPH free radicals and ferrous reduction in the FRAP method. Moreover, the reduction of inflammatory and antioxidant genes confirmed the anti‐inflammatory and antioxidant properties of NPs. These results indicate the modification of the surface of NPs in order to increase the bioavailability, stability, and effectiveness of medicinal compounds in therapeutic applications.

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“…After ensuring complete dispersion, the sample was characterized using a particle size analyzer (Vasco3, Cordouan, France). Droplet stability of PSE-PLGA-NPs was subsequently evaluated by measuring their surface charge (CAD-Zeta Compact, France) [24].…”

Section: Characterization Of Pse-plga-nps By Dls Zetapotentialmentioning

confidence: 99%

Fabrication of folic acid-conjugated chitosan-coated PLGA nanoparticles for targeted delivery of Peganum harmala smoke extract to breast cancer cells

Tabrizi

2022

Nanotechnology

Self Cite

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In this study, PLGA-NPs coated with folic acid-chitosan (PCF-NPs) loaded with Peganum harmala smoke extract (PSE) were synthesized (PSE-PCF-NPs), and their anti-cancer effects were evaluated. PSE-PCF-NPs were synthesized by the nanoprecipitation method and then characterized by DLS, SEM, and FTIR methods. HPLC and UV–vis spectroscopy were used to evaluate the PSE’s folic acid (FA) binding and encapsulation. PSE-PCF-NPs-mediated cell viability and apoptosis were investigated by MTT, qPCR, flow cytometry, AO/PI, and DAPI staining. Anti-oxidant properties of PSE-PCF-NPs were evaluated by ABTS, DPPH, FRAP, and ROS. Angiogenic effects of PSE-PCF-NPs were assessed by CAM assay. The PSE-PCF-NPs (276.16 nm, PDI: 0.25, zeta-potential: +32.31 mV, FB: 67.6% and %EE: 89%) demonstrated selective toxicity on MCF-7 cells (IC50: 75.65 μg ml−1). The occurrence of apoptosis in MCF-7 cells was confirmed by up-regulation of P53, Cas-3, and Cas-9 genes, increased SubG1 phase cells, and the results of fluorescent staining. Scavenging free radicals, reducing iron ions, increasing intracellular ROS, and decreasing SOD gene confirmed the anti- and pro-oxidant effects of PSE-PCF-NPs outside and inside MCF-7 cells. Reduction of angiogenic factors in CAM assay showed the anti-angiogenic effects of PSE-PCF-NPs. PSE-PCF-NPs, due to their anti-cancer properties, can be considered a therapeutic agent in cancer studies.

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Incorporation of Boswellia sacra essential oil into chitosan/TPP nanoparticles towards improved therapeutic efficiency

Cited by 23 publications

References 70 publications

An Ex vivo Apoptotic and Cytotoxic Effects of Frankincense on Oral Squamous Cell Carcinoma Cell Line

An Ex vivo Apoptotic and Cytotoxic Effects of Frankincense on Oral Squamous Cell Carcinoma Cell Line

Evaluation of the effect of designed PLGA‐arctiin nanoparticles modified with folic acid and chitosan on colon cancer cells

Fabrication of folic acid-conjugated chitosan-coated PLGA nanoparticles for targeted delivery of Peganum harmala smoke extract to breast cancer cells

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