Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles

Zaman, Paula; Wang, Julia; Blau, Adam; Wang, Weiping; Li, Tina; Kohane, Daniel S.; Loscalzo, Joseph

doi:10.2147/ijn.s119174

Cited by 12 publications

(9 citation statements)

References 39 publications

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“…DSCS NP were prepared according to a previously described method. 40 To increase the stability of DSCS NP in salt solutions, XDSCS NP were prepared by covalently crosslinking chitosan with succinic acid within the core of DSCS NP. DSCS NP was suspended in 100 mM HEPES buffer (pH 7.0), mixed with succinic acid (20 mM) for 1 hour, and centrifuged (at 15,000× g for 15 minutes).…”

Section: Methodsmentioning

confidence: 99%

“…The amount of un-neutralized DS in the particles was measured by Azure A assay, from which the un-neutralized glucose sulfate units in the particles were calculated. 40 Incorporation efficiencies of CNTF or OSM into xNPs were determined by SDS gel electrophoresis followed by densitometry analysis using methods as previously described. 40 …”

Section: Methodsmentioning

confidence: 99%

“… 40 Incorporation efficiencies of CNTF or OSM into xNPs were determined by SDS gel electrophoresis followed by densitometry analysis using methods as previously described. 40 …”

Section: Methodsmentioning

confidence: 99%

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Retinal Protection by Sustained Nanoparticle Delivery of Oncostatin M and Ciliary Neurotrophic Factor Into Rodent Models of Retinal Degeneration

Yang

Lü

Feng³

et al. 2021

Trans. Vis. Sci. Tech.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Retinal Protection by Sustained Nanoparticle Delivery of Oncostatin M and Ciliary Neurotrophic Factor Into Rodent Models of Retinal Degeneration

Yang

Lü

Feng³

et al. 2021

Trans. Vis. Sci. Tech.

Self Cite

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“…Due to their positive surface charges they open the possibility to fabricate polyelectrolyte complexes with anionic polymers such as glutamic acid (Fang et al 2014), hyaluronic acid (Lalevee et al 2016), dextrane sulfate (Kulkarni et al 2016), heparin (Almodovar and Kipper 2011), dermatan sulfate (Rasente et al 2016), and chondroitin sulfate (Tsai et al 2011). Particularly the presence of glycosaminoglycans, which are naturally found in extracellular matrices, is known to modulate the activity of cytokines and growth factors by binding to the polymers (Zaman et al 2016). Otherwise, chitosan-based scaffolds can be loaded with cytokines and growth factors to attract cells and stimulate tissue regeneration (Sun et al 2012a;Bader et al 2015;Choi et al 2015).…”

Section: Tissue Engineeringmentioning

confidence: 99%

Chitin/Chitosan: Versatile Ecological, Industrial, and Biomedical Applications

Merzendorfer

Cohen

2019

Biologically-Inspired Systems

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Chitin is a linear polysaccharide of N-acetylglucosamine, which is highly abundant in nature and mainly produced by marine crustaceans. Chitosan is obtained by hydrolytic deacetylation. Both polysaccharides are renewable resources, simply and cost-effectively extracted from waste material of fish industry, mainly crab and shrimp shells. Research over the past five decades has revealed that chitosan, in particular, possesses unique and useful characteristics such as chemical versatility, polyelectrolyte properties, gel-and film-forming ability, high adsorption capacity, antimicrobial and antioxidative properties, low toxicity, and biocompatibility and biodegradability features. A plethora of chemical chitosan derivatives have been synthesized yielding improved materials with suggested or effective applications in water treatment, biosensor engineering, agriculture, food processing and storage, textile additives, cosmetics fabrication, and in veterinary and human medicine. The number of studies in this research field has exploded particularly during the last two decades. Here, we review recent advances in utilizing chitosan and chitosan derivatives in different technical, agricultural, and biomedical fields.

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“…Implementation of chemoattracting factors such as SDF-1α and PDGF-BB for the recruitment of endogenous MSCs into a biomaterial matrix-filled defect can be by the simple addition of the protein into the matrix or by incorporation of the growth factor into a secondary delivery vehicle such as a nanoparticle; nanoparticles offer the potential for a greater degree of control over the sustained release of the proteins. Polyelectrolyte complex nanoparticles (PCNs), one type of nanoparticle that has been employed for protein release, is prepared from dextran sulfate (DS) and chitosan (CS) from aqueous phase [ 25 , 26 ]. PCNs have high protein encapsulation efficiency (~85%) and rapid initial release properties and may physically protect the proteins/peptides from proteinase cleavage, to serve as controlled release vehicles for growth factors.…”

Section: Introductionmentioning

confidence: 99%

Platelet-Derived Growth Factor Stimulated Migration of Bone Marrow Mesenchymal Stem Cells into an Injectable Gelatin-Hydroxyphenyl Propionic Acid Matrix

et al. 2021

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Bone marrow mesenchymal stem cells (bMSCs) are responsible in the repair of injured tissue through differentiation into multiple cell types and secretion of paracrine factors, and thus have a broad application profile in tissue engineering/regenerative medicine, especially for the musculoskeletal system. The lesion due to injury or disease may be a closed irregular-shaped cavity deep within tissue necessitating an injectable biomaterial permissive of host (endogenous) cell migration, proliferation and differentiation. Gelatin-hydroxyphenyl propionic acid (Gtn-HPA) is a natural biopolymer hydrogel which is covalently cross-linked by horseradish peroxidase (HRP) and hydrogen peroxide (H2O2) in situ and can be delivered to the lesion by needle injection. Growth factors and cytokines can be directly incorporated into the gel or into nano- and micro-particles, which can be employed for sustained release of biomolecules while maintaining their bioactivity. In this study, we selected polyelectrolyte complex nanoparticles (PCNs) prepared with dextran sulfate and chitosan as the carrier for platelet-derived growth factor (PDGF)-BB and stromal cell-derived factor (SDF)-1α, which have been tested effectively in recruiting stem cells. Our in vitro results showed a high degree of viability of bMSCs through the process of Gtn-HPA covalent cross-linking gelation. The Gtn-HPA matrix was highly permissive of bMSC migration, proliferation, and differentiation. PDGF-BB (20 ng/mL) directly incorporated into the gel and, alternatively, released from PCNs stimulated bMSC migration and proliferation. There were only small differences in the results for the direct incorporation of PDGF into the gel compared with its release from PCNs, and for increased doses of the growth factor (200 ng/mL and 2 µg/mL). In contrast, SDF-1α elicited an increase in migration and proliferation only when released from PCNs; its effect on migration was notably less than PDGF-BB. The in vitro results demonstrate that PDGF-BB substantially increases migration of bMSCs into Gtn-HPA and their proliferation in the gel, and that these benefits can be derived from incorporation of a relatively low dose of the growth factor directly into the gel. These findings commend the use of Gtn-HPA/PDGF-BB as an injectable therapeutic agent to treat defects in musculoskeletal tissues.

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Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles

Cited by 12 publications

References 39 publications

Retinal Protection by Sustained Nanoparticle Delivery of Oncostatin M and Ciliary Neurotrophic Factor Into Rodent Models of Retinal Degeneration

Retinal Protection by Sustained Nanoparticle Delivery of Oncostatin M and Ciliary Neurotrophic Factor Into Rodent Models of Retinal Degeneration

Chitin/Chitosan: Versatile Ecological, Industrial, and Biomedical Applications

Platelet-Derived Growth Factor Stimulated Migration of Bone Marrow Mesenchymal Stem Cells into an Injectable Gelatin-Hydroxyphenyl Propionic Acid Matrix

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