Incorporation of dipalmtoyl phosphatidylcholine into human plasma low density lipoproteins

Jackson, Richard L.; Cardin, Alan D.; Barnhart, Roger L.; Johnson, J D

doi:10.1016/0005-2760(80)90089-2

Cited by 19 publications

(5 citation statements)

References 6 publications

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“…The data in Figures 3 and 4 show that rapid intervesicular transfer of glucosylceramide by the transfer protein occurs only when both donor and acceptor matrix lipids are in the liquid-crystalline state. An analogous dependence of the rate of transfer on the phase state of the donor and acceptor vesicles has also been noted for the phosphatidylcholine-specific transfer proteins from bovine liver (Jackson et al, 1979(Jackson et al, , 1980 Kasper & Helmkamp, 1981;Welti & Helmkamp, 1984). In the case of the glycosphingolipid transfer protein, the data in Figure 6A show that the binding of the protein to vesicles occurs to essentially the same extent both above and below the gel-liquid-crystalline phase transition temperature, Tm.…”

Section: Discussionsupporting

confidence: 55%

Glycolipid transfer protein from bovine brain

Wong¹,

Brown²,

Barenholz³

et al. 1984

Biochemistry

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Glycolipid transfer protein from bovine brain has been purified partially by ammonium sulfate precipitation, CM-52 ion-exchange, and Sephadex G-75 column chromatography. Both pyrene-labeled and tritium-labeled glucocerebrosides have been used to study the kinetics of protein-mediated transfer between donor and acceptor vesicles. Protein accelerates glucocerebroside transfer but does not accelerate phospholipid transfer. In colyophilized small sonicated vesicles (10% glucocerebroside, 90% 1-palmitoyl-2-oleoyl-phosphatidylcholine) about two-thirds of the glycolipid is transferred in 2 h and the remaining one-third does not transfer (up to 5 h). For donor and acceptor vesicles made of dipalmitoylphosphatidylcholine or 1-palmitoyl-2-oleoyl-phosphatidylcholine, glucocerebroside (10% in donors) is transferred rapidly only when both the donor and acceptor matrix phospholipids are in the liquid-crystalline state. If either donor or acceptor vesicles are in the gel state, transfer protein mediated transfer is much reduced. The amount of transfer protein bound specifically to glucocerebroside-containing vesicles is nearly equal above and below the matrix phospholipid phase transition temperature. Bound protein transfers glucocerebroside upon addition of acceptor vesicles.

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Section: Discussionsupporting

confidence: 55%

Glycolipid transfer protein from bovine brain

Wong¹,

Brown²,

Barenholz³

et al. 1984

Biochemistry

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“…Preparation of Di[l-'4C]palmitoylphosphatidylcholine-Labeled LDL. Human plasma LDL were labeled with di [ 1 -14C]palmitoylphosphatidylcholine by using bovine liver phosphatidylcholine exchange protein as described previously (Jackson et al, 1980a); radiolabeled LDL contained 172 cpm/^tg of phospholipid.…”

Section: Methodsmentioning

confidence: 99%

Sulfhydryl chemistry and solubility properties of human plasma apolipoprotein B

Cardin¹,

Witt²,

Barnhart³

et al. 1982

Biochemistry

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105

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“…However most of these studies report changes in particle composition upon equlibrium and between lipoparticle types. Only a few studies have followed exchange events that occur between lipoproteins and cells 17 , and lipoproteins and simplified cell models such as lipid vesicles 24 , and lipid microemulsions 25,26 . The complexity of these systems, has left this work inconclusive, and on ocassions contradictory.…”

Section: Introductionmentioning

confidence: 99%

Time-resolved small-angle neutron scattering as a probe for the dynamics of lipid exchange between human lipoproteins and naturally derived membranes

Maric

Lind

Raida

et al. 2019

Sci Rep

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Atherosclerosis is the main killer in the western world. Today’s clinical markers include the total level of cholesterol and high-/low-density lipoproteins, which often fails to accurately predict the disease. The relationship between the lipid exchange capacity and lipoprotein structure should explain the extent by which they release or accept lipid cargo and should relate to the risk for developing atherosclerosis. Here, small-angle neutron scattering and tailored deuteration have been used to follow the molecular lipid exchange between human lipoprotein particles and cellular membrane mimics made of natural, “neutron invisible” phosphatidylcholines. We show that lipid exchange occurs via two different processes that include lipid transfer via collision and upon direct particle tethering to the membrane, and that high-density lipoprotein excels at exchanging the human-like unsaturated phosphatidylcholine. By mapping the specific lipid content and level of glycation/oxidation, the mode of action of specific lipoproteins can now be deciphered. This information can prove important for the development of improved diagnostic tools and in the treatment of atherosclerosis.

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Incorporation of dipalmtoyl phosphatidylcholine into human plasma low density lipoproteins

Cited by 19 publications

References 6 publications

Glycolipid transfer protein from bovine brain

Glycolipid transfer protein from bovine brain

Sulfhydryl chemistry and solubility properties of human plasma apolipoprotein B

Time-resolved small-angle neutron scattering as a probe for the dynamics of lipid exchange between human lipoproteins and naturally derived membranes

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