Incorporation of dietary triacylglycerols from olive oil and high-oleic sunflower oil into VLDL triacylglycerols of hypertensive patients

Abstract

Objectives: To establish whether the ingestion of diets enriched with olive oil or high-oleic sun¯ower oil may produce changes in the composition of VLDL triacylglycerols from hypertensive patients. It could be relevant for the uptake and metabolism of triacylglycerol-derived metabolites by extrahepatic tissues. Design: Patients were assigned to the diets in a random-order sequence. Subjects: The participants were 24 hypertensive patients recruited from a religious community. Interventions: The study was conducted over two four week periods with a four week washout period between both MUFA diets. Results: Dietary olive oil kept in balance the content of saturated fatty acids and decreased the content of arachidonic acid in VLDL triacylglycerols. HOSO diet reduced the content of palmitic acid and increased the content of linoleic acid. There was also a decrease in trioleate-glycerol and an increase in tripalmitate-glycerol of VLDL after the MUFA diets, but these effects were more pronounced in the HOSO group. Intake of olive oil decreased the content of disaturated triacylglycerols and increased the content of dioleate-containing triacylglycerols. A decrease in palmitate-dioleate-glycerol after dietary HOSO was observed. Olive oil (but not HOSO) promoted the presence of long-chain PUFA of n-3 family at the sn-2 position of VLDL triacylglycerols. Conclusions: Our data indicate that olive oil and HOSO, providing a similar concentration of MUFA (oleic acid), differ in the formation of VLDL triacylglycerols in hypertensive patients. Sponsorship: This study was supported by a grant (ALI96-0456 and OLI96-2126) from the CICYT, Spain.

Simultaneous determination of molecular species of monoacylglycerols, diacylglycerols and triacylglycerols in human very-low-density lipoproteins by reversed-phase liquid chromatography

“…Although VLDL are triacylglycerol-rich lipoproteins with relevance in the development of atherosclerosis, the study of their triacylglycerol composition has been usually faced by analysing their fatty acid or total triacylglycerol content. Only exceptionally their triacylglycerol molecular species composition has been determined [9,10,11,19,20]. In the present study we report the achievement of the simultaneous determination of molecular species of triacylglycerols, but also of diacylglycerols and monoacylglycerols, which are products of the catabolism of VLDL triacylglycerols by LPL.…”

“…In 1993, we presented for the first time the molecular species composition of VLDL underivatised triacylglycerol from human origin, analysed by gas-liquid chromatography (GLC) [9]. This method was then employed for the analysis of VLDL triacylglycerols from healthy and hypertensive subjects receiving diets rich in olive oil or high-oleic sunflower oil [10,11]. However, the analysis of triacylglycerols by GLC with flame-ionization detector (FID) presented deficiencies in the detection of the most unsaturated triacylglycerols [12].…”

Plasma lipid modifications in elderly people after administration of two virgin olive oils of the same variety (Olea europaeavar.hojiblanca) with different triacylglycerol composition

“…The oils employed in the present study were selected to present differences in this lipid class: whereas VOO1 contained a greater amount of triolein, VOO2 was significantly enriched in species containing linoleic acid (Table 2). We have previously demonstrated that linoleoyl-species of triacylglycerols from HOSO were incorporated to a greater extent into chylomicrons and VLDL compared with VOO (Ruiz-Gutierrez et al 1999;Abia et al 1999Abia et al , 2001).…”

Chylomicron and VLDL TAG structures and postprandial lipid response induced by lard and modified lard

“…The main VLDL TAG structures of people on a high oleic acid diet were dioleoylpalmitoylglycerol and linoleoyloleoylpalmitoylglycerol (in Ref. 39, co-eluted with dioleoylpalmitoleoylglycerol), both comprising more than or nearly 20% of all TAG (39,40). Of the four M.W.…”