Incorporation of Bromelain into Dermatological Bases: Accelerated Stability Studies

Abstract

This work aimed the incorporation of the enzyme bromelain from pineapple peel into dermatological bases for potential therapeutic application. The enzyme was incorporated into Lanette cream and lotion, as well as Carbopol gel and Chemyunion® cream and lotion, at a concentration of 0.5% (w/w). These bases were subjected to centrifugation test and accelerated stability test during 90 days at 25 °C (with and without sunlight), 37 °C and 4 °C, in order to evaluate bromelain stability in dermatological bases. Total protein content and enzymatic activity were measured. The bases were stable as its organoleptic characteristics (appearance, color, smell and sensitivity to touch) only when kept at 4 ºC with activity remaining 95.5%, 84,9%, 77.7%, 73.8% and 72.3%, after 90 days of testing in Carbopol gel, Lanette and Chemyunion® cream, and Lanette and Chemyunion® lotion, respectively. Based on the results, it was possible to incorporate bromelain into dermatological bases, and the activity was well preserved when these bases were kept in refrigerator at 4 ºC.

Biologically active compounds of plant origin in medicine

“…This effect is attributed to the antiviral, antibacterial, anti-inflammatory, and immunomodulatory properties of bromelain. Bresolin et al [2014] reported promising results facilitating consideration of incorporation of the enzyme bromelain from pineapple peel into dermatological bases for potential therapeutic application. They found that when the enzyme was incorporated into the Lanette cream and lotion as well as Carbopol gel and Chemyunion® cream and lotion at a concentration of 0.5% (w/w), these bases were stable when kept in a refrigerator at 4°C.…”