Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds

Lei, Yu; Huang, S. K.; Sharif-Kashani, Pooria; Chen, Yong; Kavehpour, Pirouz; Segura, Tatiana

doi:10.1016/j.biomaterials.2010.08.016

Cited by 89 publications

(109 citation statements)

References 40 publications

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“…1 showed entrapped pDNA into hydrogel in different chitosan/ PVP ratios. In many studies, naked or complexed DNA have been incorporated into hydrogel scaffolds such as chitosan and co-polymers with its PEG and other polymers (11)(12)(13)(14). Lei et al studied with the incorporation of active DNA/ PEI polyplexes into hydrogel scaffolds (14).…”

Section: Electrophoretic Studymentioning

confidence: 99%

“…In many studies, naked or complexed DNA have been incorporated into hydrogel scaffolds such as chitosan and co-polymers with its PEG and other polymers (11)(12)(13)(14). Lei et al studied with the incorporation of active DNA/ PEI polyplexes into hydrogel scaffolds (14). Tokatlian et al encapsulated VEGF gene containing plasmid into porous hyaluronic acid hydrogels to investigate scaffold-mediated local gene delivery in diabetic wound model (15).…”

Section: Electrophoretic Studymentioning

confidence: 99%

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The Effects to GM-CSF Expression and Fibroblast Proliferation of pGMCSF Containing Chitosan/PVP Hydrogels

Şalva¹,

Akbuğa²

2016

Marmara Pharmaceutical Journal

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The aim of this study was to develop pGM-CSF containing chitosan/PVP hydrogel system for gene delivery. The hydrogels in semi-interpenetrating polymer network (semi-IPN) structure were synthesized by glutaraldehyde crosslinking between 2% chitosan and 2%, %4, %6 PVP. The viscosity, swelling properties and surface morphology of hydrogels were examined. These hydrogels showed highly porous structure. The porosity was increased with enhanced PVP concentration. Viscosity decreased with increasing PVP concentration of the hydrogels. The swelling percentage increased with increasing PVP concentration. In order to examine the effect of cell attachment and proliferation of hydrogels, NIH-3T3 fibroblast cell was used. The cells were able to attach on the top surface of hydrogels. However, the attachment of cells were slow at days 1 and 3. At days 7 and 14, cells looked healthy and normal and proliferated around and top of the hydrogels. With the ELISA study, the highest GM-CSF expression was obtained with pGM-CSF containing 2% chitosan+4% PVP hydrogels. MTT assay showed that chitosan/PVP hydrogels did not induce significant cytotoxic effect. As a result, these hydrogels could be served candidate for local gene delivery in tissue regeneration.

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Section: Electrophoretic Studymentioning

confidence: 99%

Section: Electrophoretic Studymentioning

confidence: 99%

The Effects to GM-CSF Expression and Fibroblast Proliferation of pGMCSF Containing Chitosan/PVP Hydrogels

Şalva¹,

Akbuğa²

2016

Marmara Pharmaceutical Journal

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“…This can be explained by the fact that HA improves cell targeting by binding to CD44 expressed by many cell types including human MSCs at their surface. It also reduces the toxicity of PEI because the cationic amine groups from PEI are combined with the carboxylic groups present in HA resulting in higher cell viability levels 146 . The perfect synthetic polymer-based vector has yet to be found.…”

Section: Synthetic Polymer-based Transfectionsmentioning

confidence: 99%

Non-viral gene therapy for bone tissue engineering

Wegman

2013

Biotechnology and Genetic Engineering Reviews

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“…77 However, the delivery of DNA through a synthetic cationic polymer ("polyplexes") hydrogel has not previously succeeded because of high aggregation and inactivation of the cationic polymer inside the hydrogel matrix. 78 But, the same polymer hydrogel when functionalized with Fbg, could be effectively used to deliver concentrated DNA without aggregation. 62 Osathanon et al reported a novel method of constructing a calcium phosphate-Fbn hydroxyapatite-based composite scaffold, which revealed a tightly interconnected network and a more porous structure, and the biodegradable composite supported primary osteoblast-like cell adhesion, proliferation, and differentiation in bone tissue engineering, in vitro and in vivo.…”

Section: Fbg and Fbn Tubes As Vascular Graftmentioning

confidence: 99%

Fibrinogen and fibrin based micro and nano scaffolds incorporated with drugs, proteins, cells and genes for therapeutic biomedical applications

Rajangam¹,

An²

2013

IJN

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Over the past two decades, many types of natural and synthetic polymer-based micro-and nanocarriers, with exciting properties and applications, have been developed for application in various types of tissue regeneration, including bone, cartilage, nerve, blood vessels, and skin. The development of suitable polymers scaffold designs to aid the repair of specific cell types have created diverse and important potentials in tissue restoration. Fibrinogen (Fbg)-and fibrin (Fbn)-based micro-and nanostructures can provide suitable natural matrix environments. Since these primary materials are abundantly available in blood as the main coagulation proteins, they can easily interact with damaged tissues and cells through native biochemical interactions. Fbg-and Fbn-based micro and nanostructures can also be consecutively furnished/ or encapsulated and specifically delivered, with multiple growth factors, proteins, and stem cells, in structures designed to aid in specific phases of the tissue regeneration process. The present review has been carried out to demonstrate the progress made with micro and nanoscaffold applications and features a number of applications of Fbg-and Fbn-based carriers in the field of biomaterials, including the delivery of drugs, active biomolecules, cells, and genes, that have been effectively used in tissue engineering and regenerative medicine.

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Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds

Cited by 89 publications

References 40 publications

The Effects to GM-CSF Expression and Fibroblast Proliferation of pGMCSF Containing Chitosan/PVP Hydrogels

The Effects to GM-CSF Expression and Fibroblast Proliferation of pGMCSF Containing Chitosan/PVP Hydrogels

Non-viral gene therapy for bone tissue engineering

Fibrinogen and fibrin based micro and nano scaffolds incorporated with drugs, proteins, cells and genes for therapeutic biomedical applications

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