Incorporation of a silicon-based polymer to PEG-DA templated hydrogel scaffolds for bioactivity and osteoinductivity

Frassica, Michael T.; Jones, Sarah K.; Díaz‐Rodríguez, Patricia; Hahn, Mariah S.; Grunlan, Melissa A.

doi:10.1016/j.actbio.2019.09.018

Cited by 28 publications

(36 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…106,107 Our group has demonstrated that bioactivity and osteoinductivity can also be achieved with nonbrittle materials by the incorporation of a silicon-based, inorganic polymer [star-polydimethylsiloxane methacrylate] into poly(ethylene glycol) diacrylate (PEG-DA) hydrogels. 88,89 The instructive behavior of this siliconbased polymer is attributed to the known role that silicon plays in bone mineralization and gene activation 108,109 which had originally prompted the use of certain glasses, ceramics, and nanosilicates. As described later, fabrication strategies have been utilized to maximize the potential of these materials by controlling morphological features of the scaffold.…”

Section: ■ Considerations Formentioning

confidence: 99%

“…Osteoinductivity is broadly understood as the ability to induce bone formation via cellular differentiation to a bone-forming lineage. Mechanisms for this action have been widely studied, including active facilitation of nutrient and waste transport via vascularization, , adherence of progenitor cells on the material surface (via protein adsorption or direct integrin binding) and subsequent interaction with surface properties, ,− osteogenic response to calcium and phosphate ions, − and concentration of osteogenic proteins by material affinity. ,, Osteoinductivity is often affiliated with a certain chemical composition (e.g., hydrophobic and inorganic materials such as bioglasses, calcium phosphates, siloxanes, and nanosilicates) ,− and pore topography (e.g., mesopores, , rough surfaces, and aligned fibers). Matrix stiffness is also identified as a key factor in osteogenesis, as adhered cells can respond to the local environment through a variety of mechanisms. , Generally, stiffer matrices have been identified as osteogenic; however, other simultaneous cell–material interactions add complexity to a strict correlative relationship.…”

Section: Considerations For Osteochondral-instructive Scaffoldsmentioning

confidence: 99%

See 1 more Smart Citation

Perspectives on Synthetic Materials to Guide Tissue Regeneration for Osteochondral Defect Repair

Frassica

Grunlan

2020

ACS Biomater. Sci. Eng.

Self Cite

View full text Add to dashboard Cite

Regenerative engineering holds the potential to treat clinically pervasive osteochondral defects (OCDs). In a synthetic materials-guided approach, the scaffold’s chemical and physical properties alone instruct cellular behavior in order to effect regeneration, referred to herein as “instructive” properties. While this alleviates the costs and off-target risks associated with exogenous growth factors, the scaffold must be potently instructive to achieve tissue growth. Moreover, toward achieving functionality, such a scaffold should also recapitulate the spatial complexity of the osteochondral tissues. Thus, in addition to the regeneration of the articular cartilage and underlying cancellous bone, the complex osteochondral interface, composed of calcified cartilage and subchondral bone, should also be restored. In this Perspective, we highlight recent synthetic-based, instructive osteochondral scaffolds that have leveraged new material chemistries as well as innovative fabrication strategies. In particular, scaffolds with spatially complex chemical and morphological features have been prepared with electrospinning, solvent-casting–particulate-leaching, freeze-drying, and additive manufacturing. While few synthetic scaffolds have advanced to clinical studies to treat OCDs, these recent efforts point to the promising use of the chemical and physical properties of synthetic materials for regeneration of osteochondral tissues.

show abstract

Section: ■ Considerations Formentioning

confidence: 99%

Section: Considerations For Osteochondral-instructive Scaffoldsmentioning

confidence: 99%

Perspectives on Synthetic Materials to Guide Tissue Regeneration for Osteochondral Defect Repair

Frassica

Grunlan

2020

ACS Biomater. Sci. Eng.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Therefore, MSCs are believed to be an important stem cell for tissue engineering, as well as being capable of differentiating into various specialized tissues, including bone, tendons, cartilage, muscle, ligaments, and fat [ 54 , 55 ]. The fabrication of macroporous PEG hydrogel scaffolds for use as a bioactive material for human bone marrow-derived MSCs in osteogenic differentiation has been successfully reported [ 56 ]. Another report also demonstrated that amphiphilic poly (d, l-lactic acid)-co-poly (ethylene glycol)-co-poly (d, l-lactic acid) (PELA) with HA, fabricated by electrospinning, can lead to osteogenic differentiation of MSCs [ 57 ].…”

Section: Introductionmentioning

confidence: 99%

Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells

et al. 2021

View full text Add to dashboard Cite

In this study, polyethylene glycol (PEG) with hydroxyapatite (HA), with the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The surface morphology and chemical composition were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), UV–Vis spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and contact angle assessment. The effects of PEG-HA-AuNP nanocomposites on the biocompatibility and biological activity of MC3T3-E1 osteoblast cells, endothelial cells (EC), macrophages (RAW 264.7), and human mesenchymal stem cells (MSCs), as well as the guiding of osteogenic differentiation, were estimated through the use of an in vitro assay. Moreover, the anti-inflammatory, biocompatibility, and endothelialization capacities were further assessed through in vivo evaluation. The PEG-HA-AuNP nanocomposites showed superior biological properties and biocompatibility capacity for cell behavior in both MC3T3-E1 cells and MSCs. These biological events surrounding the cells could be associated with the activation of adhesion, proliferation, migration, and differentiation processes on the PEG-HA-AuNP nanocomposites. Indeed, the induction of the osteogenic differentiation of MSCs by PEG-HA-AuNP nanocomposites and enhanced mineralization activity were also evidenced in this study. Moreover, from the in vivo assay, we further found that PEG-HA-AuNP nanocomposites not only facilitate the anti-immune response, as well as reducing CD86 expression, but also facilitate the endothelialization ability, as well as promoting CD31 expression, when implanted into rats subcutaneously for a period of 1 month. The current research illustrates the potential of PEG-HA-AuNP nanocomposites when used in combination with MSCs for the regeneration of bone tissue, with their nanotopography being employed as an applicable surface modification approach for the fabrication of biomaterials.

show abstract

“…Therefore, the ratio of AA and NIPAM would be one of the vital parameters in synthesizing the dual sensitivity of hydrogel, and it is necessary to be further investigated. Furthermore, another appropriate approach for enhancing the swelling capacity of hydrogel is to modify the hydrogel with inorganic materials, such as silicon 15,16 or silica 17,18 . It can improve the swelling performance and simultaneously increase the mechanical strength of the hydrogel 19 .…”

Section: Introductionmentioning

confidence: 99%

Synthesis of dual pH‐ and temperature‐sensitive poly(N‐isopropylacrylamide‐co‐acrylic acid)/sewage sludge ash hydrogel with the simultaneously high performance of swelling and deswelling

Pan

Huang

Guo

et al. 2021

Polymers for Advanced Techs

View full text Add to dashboard Cite

An advanced dual pH‐ and temperature‐sensitive hydrogel (NASH2.5) was optimally synthesized through modification of N‐isopropylacrylamide (NIPAM) hydrogel with introducing 5 mol% acrylic acid (AA) and 2.5 wt% sewage sludge ash (SSA). The swelling kinetic results showed that NASH2.5 exhibited both high equilibrium swelling ratio and swelling rate, which was attributed to the higher porous structure as shown in scanning electron microscope, and the more hydrogen bonding formed inside of the hydrogel as investigated in Fourier transform infrared spectrometer. In addition, its curve was better fitted to the pseudo‐second‐order model, indicating that the water absorption process was dominated by chemisorption through forming the hydrogen bonding among the water molecules and carboxyl/silanol groups of the hydrogel. Compared with the pure NIPAM hydrogel, the water transport mechanism switched from Case I diffusion to Case II diffusion by introduction of AA and further SSA. Furthermore, through the results of the deswelling kinetics in pH value change (from 9 to 4 and 2, respectively), temperature value change (from 25 to 40, 50, and 60°C, respectively), and dual pH and temperature values changes, NASH2.5 not only presented a high pH sensitivity, but also showed high sensitive to temperature by achieving high water recovery ratio in rapid dehydrated rate. Therefore, the dual stimuli‐sensitive hydrogel with the simultaneously high performance of swelling and deswelling would provide a suitable alternative for specific applications such as pollutant adsorption.

show abstract

Incorporation of a silicon-based polymer to PEG-DA templated hydrogel scaffolds for bioactivity and osteoinductivity

Cited by 28 publications

References 42 publications

Perspectives on Synthetic Materials to Guide Tissue Regeneration for Osteochondral Defect Repair

Perspectives on Synthetic Materials to Guide Tissue Regeneration for Osteochondral Defect Repair

Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells

Synthesis of dual pH‐ and temperature‐sensitive poly(N‐isopropylacrylamide‐co‐acrylic acid)/sewage sludge ash hydrogel with the simultaneously high performance of swelling and deswelling

Contact Info

Product

Resources

About