Incorporation of a RAM cell in a microcolumn for on-column detection of radiolabeled molecules for LC-RAM-ESI-MS

Schultz, Gary; Alexander, James N.

doi:10.1002/(sici)1520-667x(1998)10:5<431::aid-mcs6>3.0.co;2-h

Cited by 9 publications

(3 citation statements)

References 3 publications

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“…Recently, we have enhanced our nanoscale detection capabilities with the development of three micro-detection schemes for micro-LC that include ESI-MS, 21 radioactivity monitoring 22 and micro-evaporative light scattering detection. 23 The objective of this study was to develop a simple and robust sheathless interface that can be used with either nano-LC/ESI-MSI or CE/ESI-MS.…”

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confidence: 99%

Development of a nano-electrospray mass spectrometry source for nanoscale liquid chromatography and sheathless capillary electrophoresis

Alexander

Schultz

Poli

1998

Rapid Commun. Mass Spectrom.

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A Fisons Quattro I electrospray ionization (ESI) source has been modified to produce stable electrospray ion currents at flow rates as low as 50 nL/min. The original counter electrode and skimmer cone lens of the Fisons ESI source have been replaced with a spherical cone lens. This improved source provides a greater range of x,y,z positioning of a stainless steel tip resulting in a stable ion signal for flow rates of 50 nL/min to 2 mL/min. A tapered stainless steel electrospray tip (50 mm i.d.) was evaluated for mass spectrometry using nano-liquid chromatography (50 mm i.d., flow rate = 120 nL/min) and sheathless capillary electrophoresis. The analysis of a nonionic surfactant, octylphenol ethoxylate, was accomplished with both nanoscale separation techniques. #

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confidence: 99%

Development of a nano-electrospray mass spectrometry source for nanoscale liquid chromatography and sheathless capillary electrophoresis

Alexander

Schultz

Poli

1998

Rapid Commun. Mass Spectrom.

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“…Schultz and Alexander developed an on-column RAM solid scintillation cell for packed LC microcolumns (250 μm i.d.) combined with ESI-MS (LC-RAM-ESI-MS) [10]. They showed that band broadening and RAM sensitivity depended on the particle size of the solid scintillant, cell volume, and cell diameter.…”

Section: Radiolabeled Parent Drugmentioning

confidence: 99%

New analytical strategies in studying drug metabolism

Staack

Hopfgartner

2007

Anal Bioanal Chem

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Identification and elucidation of the structures of metabolites play major roles in drug discovery and in the development of pharmaceutical compounds. These studies are also important in toxicology or doping control with either pharmaceuticals or illicit drugs. This review focuses on: new analytical strategies used to identify potential metabolites in biological matrices with and without radiolabeled drugs; use of software for metabolite profiling; interpretation of product spectra; profiling of reactive metabolites; development of new approaches for generation of metabolites; and detection of metabolites with increased sensitivity and simplicity. Most of the new strategies involve mass spectrometry (MS) combined with liquid chromatography (LC).

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“…The method requires fabrication of a packed capillary column with incorporation of the radiochemical cell within the custom-made column [9]. We herein report a much more simple solution to this analytical problem which requires no custom-made hardware.…”

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Radiochemical detection for packed capillary liquid chromatography-mass spectrometry

Onisko

2002

J. Am. Soc. Mass Spectrom.

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For the identification of trace level organic molecules, such as drug or pesticide metabolites, there is need of a practical method to do packed capillary liquid chromatography-mass spectrometry (LC-MS) with radiochemical detection. This problem has been successfully solved by use of a post column flow-splitter, with coaxially transported makeup flow that increases the split flow rate to a flow compatible with commercially available radiochemical flow cells. To test the device, 14 C-labeled azoxystrobin, a commercial fungicide, was analyzed by liquid chromatography-radiochemical activity monitor-mass spectrometry (LC-RAM-MS) using a 0.32 mm i.d. packed capillary column. Azoxystrobin could be detected at 500 pCi with good signal/noise. The method is general and can be used with capillary LC columns of smaller diameters. Column efficiency of about 20,000 theoretical plates/m was achieved using either radiochemical or mass spectrometric data, thus demonstrating the lack of band broadening using the described method for radiochemical detection. The simple hardware described allows the routine use of packed capillary LC with radiochemical detection. (J Am Soc Mass Spectrom 2002, 13, 82-84)

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Incorporation of a RAM cell in a microcolumn for on-column detection of radiolabeled molecules for LC-RAM-ESI-MS

Cited by 9 publications

References 3 publications

Development of a nano-electrospray mass spectrometry source for nanoscale liquid chromatography and sheathless capillary electrophoresis

Development of a nano-electrospray mass spectrometry source for nanoscale liquid chromatography and sheathless capillary electrophoresis

New analytical strategies in studying drug metabolism

Radiochemical detection for packed capillary liquid chromatography-mass spectrometry

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