Incorporation of 32P-labelled intermediates into the phospholipids of cell-free preparations of rat brain

McMurray, W. C.; Strickland, K. P.; Berry, James F.; Rossiter, R. J.

doi:10.1042/bj0660634

Cited by 97 publications

(20 citation statements)

References 26 publications

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“…Choline can be incorporated into phosphatidylcholine in brain homogenates by synthesis dr novo involving cytidine nucleotides or by Ca*+-stimulated base exchange (McMurray et al, 1957;Kanfer, 1972;Miller and Dawson, 1972). Our data suggest that oligodendrocyte homogenates can also utilise both these routes for choline incorporation and that, in The absence of added cofactors, sufficient Ca2+ is present to stimulate significant base exchange and to inhibit strongly CTP-dependent choline incorporation.…”

Section: Discussionmentioning

confidence: 81%

Incorporation of Choline and Inositol into Phospholipids of Isolated Bovine Oligodendrocyte Perikarya

Brammer

Carey

1980

Journal of Neurochemistry

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Oligodendrocyte perikarya have been prepared from fresh and frozen bovine corpus callosum by a slight modification of previously published methods. The properties of the cells which we have obtained appear to be very similar to those previously described in other laboratories. Oligodendrocyte homogenates have been incubated with radioactive choline and inositol in the presence of a number of possible cofactors of phospholipid synthesis. Choline was incorporated into oligodendrocyte phosphatidylcholine both by synthesis de novo and by Ca2+-stimulated base exchange. There was no detectable incorporation of choline into sphingomyelin by either of these routes. Inositol was incorporated into oligodendrocyte phosphatidylinositol by synthesis de novo and by Mn2+-stimulated exchange. Oligodendrocyte homogenates were found to contain sufficient endogenous Ca2+ both to stimulate significant incorporation of choline into lecithin by base exchange and to produce considerable inhibition of lecithin synthesis de novo.

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Section: Discussionmentioning

confidence: 81%

Incorporation of Choline and Inositol into Phospholipids of Isolated Bovine Oligodendrocyte Perikarya

Brammer

Carey

1980

Journal of Neurochemistry

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“…and tissue the medium was identical with that in Fractionation of the phospholipids formed on incubation of labelled nucleotides with brain cortex. There was no reason to suppose that 32P-labelled phospholipids formed from CDP-ethanolamine or CDP-choline in brain homogenates would be other than phosphatidylethanolamine (or the corresponding plasmalogen) and phosphatidylcholine respectively (McMurray, Strickland, Berry & Rossiter, 1957). It was, however, decided to identify the lipids formed from the CDP esters of monomethylaminoethanol and dimethylaminoethanol.…”

Section: Miboellaneous Method8mentioning

confidence: 99%

The incorporation of the phosphate esters of N-substituted aminoethanols into the phospholipids of brain and liver

Ansell¹,

Chojnacki²

1966

Biochemical Journal

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1. The phosphate esters of dimethylaminoethanol and monomethylaminoethanol can be incorporated from their cytidine diphosphate esters into the phospholipids of brain and liver dispersions; the deoxycytidine nucleotides of the same bases are less effective precursors. 2. The cytidylyltransferases of brain and liver are less effective in forming the cytidine diphosphate esters of monomethylaminoethanol and dimethylaminoethanol than those of ethanolamine and choline.

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“…DLd-glycerophosphate (1-GP) was prepared from 1-glyeerochlorhydrin according to the method of McMurray et al (68), and 2glycerophosphate (2-GP) was obtained commercially (Fisher Scientific Company, Toronto, Ont.). DLd-glycerophosphate (1-GP) was prepared from 1-glyeerochlorhydrin according to the method of McMurray et al (68), and 2glycerophosphate (2-GP) was obtained commercially (Fisher Scientific Company, Toronto, Ont.).…”

Section: ~Xperimenta~ Procedures and L~esultsmentioning

confidence: 99%

Glyceride isomerizations in lipid chemistry

Serdarevich

1967

J Americ Oil Chem Soc

144

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Isomerization of isopropy]idene glycerol ketals and benzylidene glycerol acetals was studied, and isomerization equilibria were established. Reaction of benzaldehyde with glycerol gave four benzylidene glycerol isomers, which were separated by column chromatography and characterized by NMR spectroscopy and other methods.Isomerization of 1-and 2-monoglycerides and of 1,2-and 1,3-diglyeerides, and their separation by column chromatography, are described. Mechanisms of isomerization in mono-and diglycerides and factors which affect them are discussed.Isomerization of 1-and 2-glycerophosphates and of cyclic glycerophosphates by acid and base was also studied. Hydrolysis products of L-3glyeerylphosphorylcholine and 2-glycerylphosphorylcholine were separated by column chromatography and characterized by periodic acid oxidation, optical rotation, and NMR spectroscopy. No isomerization of unhydrolyzed L-3glycerylphosphoryleholine and 2-glyccrylphosphorylcholine was observed. Evidence indicated that acid-catalyzed hydrolyses of phosphoglyeerides are under thermodynamic control whereas most base-catalyzed hydrolyses are under kinetic control.

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Incorporation of 32P-labelled intermediates into the phospholipids of cell-free preparations of rat brain

Abstract: * Studies carried out during the tenure of a Lederle Medical Faculty Award.

Cited by 97 publications

References 26 publications

Incorporation of Choline and Inositol into Phospholipids of Isolated Bovine Oligodendrocyte Perikarya

Incorporation of Choline and Inositol into Phospholipids of Isolated Bovine Oligodendrocyte Perikarya

The incorporation of the phosphate esters of N-substituted aminoethanols into the phospholipids of brain and liver

Glyceride isomerizations in lipid chemistry

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