1997
DOI: 10.1016/s0040-4039(97)01392-0
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Incorporation of 2-C-Methyl-d-erythritol, a Putative Isoprenoid Precursor in the Mevalonate-Independent Pathway, into Ubiquinone and Menaquinone of Escherichia coli

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Cited by 100 publications
(77 citation statements)
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“…Oligonucleotide primers and linkers were synthesized by the Protein/DNA Core Facility of the Utah Regional Cancer Center. ME was synthesized by the procedure of Duvold et al (8). DXP and 1-deoxy-D-xylulose (DXS) were synthesized by the procedure of Blagg and Poulter (3).…”
Section: Materials Sodium [2-mentioning
confidence: 99%
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“…Oligonucleotide primers and linkers were synthesized by the Protein/DNA Core Facility of the Utah Regional Cancer Center. ME was synthesized by the procedure of Duvold et al (8). DXP and 1-deoxy-D-xylulose (DXS) were synthesized by the procedure of Blagg and Poulter (3).…”
Section: Materials Sodium [2-mentioning
confidence: 99%
“…A 25-l portion was loaded onto a silica column as described above for DXS. The radioactivity in [2][3][4][5][6][7][8][9][10][11][12][13][14] C]DXS was measured as described above. Initial velocities were determined from the slope of a plot of product versus time.…”
Section: Vol 183 2001 Two R Capsulatus Dxp Synthase Genes Dxsa Anmentioning
confidence: 99%
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“…1). ME could only be used for incorporation into bacteria (Duvold et al, 1997;Kuzuyama et al, 1999;Fontana et al, 2001) and was found toxic to plant cells (Hemmerlin et al, 2003a), though the free tetrol or the corresponding lactone was shown present in many plants ( Dittrich and Angyal, 1988). While there is only weak evidence that DX has a functional role in vivo, it was already substantially used as a stable or radioactive isotope-labeled precursor for studies of the biosynthetic pathway in plants (Schwender et al, 1997;Sagner et al, 1998;Arigoni et al, 1999;Hoeffler et al, 2002;Luan and Wü st, 2002), as well as in bacteria (Hill et al, 1989;Broers, 1994;Giner et al, 1998;Rosa-Putra et al, 1998b;Spiteller et al, 2002).…”
mentioning
confidence: 99%
“…strain CL190 (7). In the second step, the intramolecular rearrangement of DXP was assumed to give a hypothetical rearrangement product, 2-C-methylerythrose 4-phosphate, which was then converted to 2-C-methyl-D-erythritol 4-phosphate (MEP) by an unspecified reduction process (8,9). We have succeeded in the first cloning and overexpression of the gene (dxr, formerly yaeM) encoding the DXP reductoisomerase from E. coli and have shown that the recombinant enzyme catalyzed the formation of MEP from DXP in a single step in the presence of both NADPH and a divalent cation, such as Mn 2ϩ , Mg 2ϩ , or Co 2ϩ (10,11).…”
mentioning
confidence: 99%