Incorporation and structural arrangement of microemulsion droplets in cylindrical pores of mesoporous silica

Protein Condensation at Nanopore Entrances as Studied by Differential Scanning Calorimetry and Small-Angle Neutron Scattering

“…The total neutron scattering intensity for a mesoporous silica with an adsorbate is described by the sum of intensities of various components, such as Bragg scattering, diffuse scattering, Porod scattering, and incoherent background (Figure S7C). The Bragg scattering component involves form and structure factors of adsorbate inside pores. ,, The diffuse scattering components are due to structural inhomogeneities of mesoporous silica and the inhomogeneity of complex fluid inside the mesoporous silica pores . We ascribed the sharp peak as (100) Bragg diffraction peak for the hexagonal pore arrangement (Type II in Figure B), because the position and width of the sharp peak were consistent with those for MPS77 in D 2 O (Figure C,G).…”

“…The slight difference between the experimental and calculated profiles is ascribed to the contribution of Porod scattering for ambiguous particle morphology and irregularities on the surfaces of MPS particles (Figure S6) because the Mb molecules within the particle reflect those ambiguous MPS structures . Diffuse scattering due to inhomogeneity of the Mb distribution also contributes the difference between the experimental and calculated profiles. ,, It is therefore concluded that the CM-SANS profiles for MPS42 and MPS90 are mainly due to neutron scattering from spherical Mb molecules randomly dispersed within MPS (Type I in Figure B).…”

“…25 Diffuse scattering due to inhomogeneity of the Mb distribution also contributes the difference between the experimental and calculated profiles. 19,29,30 It is therefore concluded that the CM-SANS profiles for MPS42 and MPS90 are mainly due to neutron scattering from spherical Mb molecules randomly dispersed within MPS (Type I in Figure 4B).…”

“…The Bragg scattering component involves form and structure factors of adsorbate inside pores. 19,25,29 The diffuse scattering components are due to structural inhomogeneities of mesoporous silica 19 and the inhomogeneity of complex fluid inside the mesoporous silica pores. 29 We ascribed the sharp peak as (100) Bragg diffraction peak for the hexagonal pore arrangement (Type II in Figure 4B), because the position and width of the sharp peak were consistent with those for MPS77 in D 2 O (Figure 3C,G).…”

“…19,25,29 The diffuse scattering components are due to structural inhomogeneities of mesoporous silica 19 and the inhomogeneity of complex fluid inside the mesoporous silica pores. 29 We ascribed the sharp peak as (100) Bragg diffraction peak for the hexagonal pore arrangement (Type II in Figure 4B), because the position and width of the sharp peak were consistent with those for MPS77 in D 2 O (Figure 3C,G). Since the DSC result indicated a concentration of Mb molecules at the pore entrances (Figure 2G), we assumed a column of condensed Mb inside the pores of MPS77 to analyze the sharp Bragg peak (Figure S7A,B).…”