Dairy products are an excellent source of lactose, water-soluble vitamins, proteins and minerals. They are not considered a key source of bioactive compounds, despite their positive nutritional benefits. The main objective of this study was to develop yogurt spread fortified with Spirulina platensis (SP) and to valorize its impact on the physicochemical properties, shelf life, and quality assessment of the yogurt spread during storage yogurt spread at 0, 14, and 28 d. SP is threadlike blue-green cyanobacteria or microalgae containing polyphenols, phytochemicals like flavonoids and carotenoids which act as antioxidants. Yogurt was developed by using low-fat milk fortified with SP by 1.5, 2.5, and 3.5% respectively to assess the shelf life of spread. The acquired data was inspected by using two-factor factorial designs (two-way analysis of variance) and LSD (Least significant difference) test was used for relative comparison of treatments. Physicochemical characteristics in yogurt spread were 75.6% moisture, 0.68% ash, 4.20 pH, 1.16% acidity, and 0.24mg of iron. Sensory evaluation of yogurt spread was done at different storage periods (0, 14, and 28 d). The current study revealed that SP can be fortified in yogurt spread for utilization of its bioactive substance’s benefits which in return can boost human health.