Incongruent plastid and nuclear DNA phylogenies reveal ancient intergeneric hybridization in Pilosella hawkweeds (Hieracium, Cichorieae, Asteraceae)

Reconstruction of phylogenetic relationships in a highly reticulate group with deep coalescence and recent speciation (Hieracium, Asteraceae)

“…TrnV-ndhC (obtained in this study) and trnT-trnL sequences from Fehrer et al (2009) were concatenated; the combined dataset was used for phylogenetic analyses. Indels were coded as single characters as described in Fehrer et al (2007); length variation in poly-A regions was omitted. The alignment containing the indel coding is provided in Supplementary File 1.…”

“…Fehrer et al (2007) have inferred an ancient intergeneric hybridization event between Hieracium and Pilosella based on incongruent nuclear and chloroplast data. Hieracium intybaceum nrDNA was so divergent from Hieracium and related genera that this taxon was suitable as an outgroup for phylogenetic analyses of nrDNA datasets ).…”

“…Since then, a large number of studies using many unlinked markers have been published, but gene tree incongruence has been found to be a common phenomenon (Degnan and Rosenberg, 2009), which seems to intensify as the number of markers increases (Rokas and Carroll, 2006). One cause of such incongruence is hybridization (for example, Linder and Rieseberg, 2004;Fehrer et al, 2007), which is one of the major processes in the evolution of vascular plants (Arnold, 1997) and is increasingly being recognized as important for animals as well. Allopolyploidy, which is merging genomes from different species, is frequently involved in hybrid speciation (Rieseberg and Willis, 2007) and can further complicate phylogenetic inference.…”

“…Altogether, 61 Hieracium individuals from 47 species included in the study of Fehrer et al (2009) were analyzed. Hispidella and diploid Pilosella species were chosen as outgroups based on Fehrer et al (2007) and Krak et al (2012). DNA extracts from the previous studies were used.…”

Novelties on the distribution of Lepechinia flammea (Lamiaceae), rediscovery of L. glomerata in Jalisco, Mexico, and their phylogenetic position within Lepechinia

“…However, the sister relationship between L. flammea and L. nelsonii in the nDNA tree (albeit only moderately supported) is somewhat surprising based on a priori expectations. The conflicting topologies between genetic compartments may be indicative of chloroplast capture or hybridization as has been suggested or documented for other core eudicots (Rieseberg and Soltis, 1991;Fehrer et al, 2007;Acosta and Premoli, 2010;Salmaki et al, 2013;Wang et al, 2013;Scheunert and Heubl, 2014;Wan et al, 204) The similarity in flower color (and size) between Lepechinia nelsonii and L. flammea may be especially important because it could facilitate hybridization between the two species. Lepechinia nelsonii is hummingbird pollinated, and given the size and color of L. flammea it may be as well.…”