SUMMARYThe frequency of anti-Toxocara spp. antibodies in individuals
attended by the Centro de Salud Familiar in the coastal Niebla town,
Chile, was related to the host and to environmental factors. IgG anti-
Toxocara antibodies were detected with a commercial ELISA kit
(SCIMEDX Corporation, USA). Samples with undetermined absorbance values were
subjected to an additional ELISA standardized by the Instituto de Salud
Pública, Chilean Health Ministry, a commercial ELISA (NOVATEC, Germany),
and a commercial Western blot kit (LDBio Diagnostics, France). Hematological exams
were performed using an automated blood counter and blood smears. Dog feces were
collected from the ground along the main road in Niebla, including rural and urban
locations. Ninety (25.4%) of the 355 examined individuals were positive by the ELISA
test. The frequency of anti-Toxocara antibodies and the infection
risk were significantly higher (p < 0.05) among those individuals
≥ 40 years old with respect to the 20-39 years old group, in individuals from rural
locations, those who did not have a safe drinking water supply in the house or who
presented blood eosinophilia. The proportion of positive samples of dog feces and the
mean number of Toxocara canis eggs/g of feces in urban and rural
areas were similar (p > 0.05).