Incompatibility Groups of Naphthalene Degradative Plasmids in Pseudomonas

Boronin, A. M.; Кочетков, В. В.; Skryabin, G. K.

doi:10.1111/j.1574-6941.1980.tb01636.x

Cited by 28 publications

(9 citation statements)

References 11 publications

(1 reference statement)

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“…The genetic information for the degradation of naphthalene by bacteria is often carried by plasmids (2,6,7,10,14,15,39 (5,11). Nevertheless, some distinct differences between the two pathways exist.…”

Section: Resultsmentioning

confidence: 99%

Purification and characterization of a 1,2-dihydroxynaphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids

et al. 1991

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1,2-Dihydroxynaphthalene dioxygenase was purified to homogeneity from a bacterium that degrades naphthalenesulfonic acids (strain BN6). The enzyme requires Fe2" for maximal activity and consists of eight identical subunits with a molecular weight of about 33,000. Analysis of the NH2-terminal amino acid sequence revealed a high degree of homology (22 of 29 amino acids) with the NH2-terminal amino acid sequence of 2,3-dihydroxybiphenyl dioxygenase from strain Pseudomonas paucimobiis Ql. 1,2-Dihydroxynaphthalene dioxygenase from strain BN6 shows a wide substrate specificity and also cleaves 5-, 6-, and 7-hydroxy-1,2-dihydroxynaphthalene, 2,3-and 3,4-dihydroxybiphenyl, catechol, and 3-methyl-and 4-methylcatechol. Similar activities against the hydroxy-1,2-dihydroxynaphthalenes were also found in cell extracts from naphthalenedegrading bacteria.Amino-and hydroxynaphthalenesulfonic acids (ANS and HNS, respectively) serve as building blocks for the largescale synthesis of azo dyes. Since arylsulfonates are very rare among natural compounds (21), naphthalenesulfonic acids are referred to as xenobiotics. Arylsulfonates are major pollutants of the environment, and the contamination of the Rhine River by sulfur-organic compounds is largely due to this class of compounds (25). Nevertheless, bacteria which degrade naphthalenesulfonic acids have repeatedly been isolated (4, 5, 29-31, 41, 43). Brilon et al. (4, 5) selected pseudomonads which degraded naphthalene-2-sulfonic acid (2NS) and naphthalene-1-sulfonic acid (1NS). The initial reaction in the degradation of iNS and 2NS is catalyzed by a 1,2-dioxygenase which weakens the carbon-sulfur bond.

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Section: Resultsmentioning

confidence: 99%

Purification and characterization of a 1,2-dihydroxynaphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids

et al. 1991

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“…These include the nah plasmids of P. putida strains PpG63 (ATCC17,484) (9) and PpG90 (ATCC17,483) (8,9), the nah plasmids studied by Skryabin et aL (45) and Boronin et aL (46), and the sal plasmids characterized by Farrell (9), Heinaru et aL (8), and Zuniga et aL (44). The restriction and restriction hybridization patterns and the genetic analyses have revealed significant sequence homology between several ofthese plasmids and nah7.…”

Section: Methodsmentioning

confidence: 99%

Plasmid gene organization: naphthalene/salicylate oxidation.

Yen¹,

Gunsalus²

1982

Proc. Natl. Acad. Sci. U.S.A.

242

160

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Genes for naphthalene metabolism are localized on nah7, an 83-kilobase (kb) plasmid, in two gene clusters under salicylate control. Polar mutations formed by insertion of the transposon Tn5 permit detection ofthe transcription direction and the gene organization within two ""10-kb DNA segments separated by a -7-kb regulatory gene region. The gene cluster specifying conversion of naphthalene to salicylate lies near the left initiation of a 25-kb DNA fragment A released by EcoRI; that for the salicylate pathway via catechol meta-fission lies near the right terminus with extension into the adjoining 5.9-kb fragment C. The genetic organization and regulation resemble the tol plasmid-encoded "upper" and "lower" pathways of toluene/xylene oxidation in Pseudomonas putida mt2.

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“…The Luria‐Bertani (LB) [7] and minimal M9 media were used for cultivation of P. putida [7]. Strain P. putida BS394 [8] was used as a donor of naphthalene degradative plasmids pBS2 [9] and pBS101 [10]. Strain Escherichia coli JM109 (Promega, USA) [7] was cultured in LB or M9 medium supplemented with (μg ml −1 ): 0.05 thiamine and 0.2 proline; the antibiotics were added at the concentrations of 50 μg ml −1 ampicillin and 40 μg ml −1 gentamicin.…”

Section: Methodsmentioning

confidence: 99%

Involvement of naphthalene dioxygenase in indole-3-acetic acid biosynthesis byPseudomonas putida

Mordukhova

Sokolov

Кочетков

et al. 2000

FEMS Microbiology Letters

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Two variants of plant growth-promoting strain Pseudomonas putida BS1380 harboring the naphthalene degradative plasmid pBS2 and the recombinant plasmid pNAU64 that contains the genes encoding for naphthalene dioxygenase were constructed by conjugation. The ability of this strain to produce phytohormone indole-3-acetic acid from different carbon sources was studied. Indole-3-acetic acid synthesis by these transconjugants was 15-30 times as much in contrast to a wild-type strain with glucose as the sole carbon source. No difference was observed in other carbon or nitrogen sources. It is suggested that naphthalene dioxygenase is involved in the conversion of indole-3-pyruvic acid to indole-3-acetic acid.

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Incompatibility Groups of Naphthalene Degradative Plasmids in Pseudomonas

Cited by 28 publications

References 11 publications

Purification and characterization of a 1,2-dihydroxynaphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids

Purification and characterization of a 1,2-dihydroxynaphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids

Plasmid gene organization: naphthalene/salicylate oxidation.

Involvement of naphthalene dioxygenase in indole-3-acetic acid biosynthesis byPseudomonas putida

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