Incompatibility Group I1 (IncI1) Plasmids: Their Genetics, Biology, and Public Health Relevance

Foley, Steven L.; Kaldhone, Pravin; Ricke, Steven C.; Han, Jing

doi:10.1128/mmbr.00031-20

Cited by 33 publications

(29 citation statements)

References 207 publications

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“…We additionally analyzed the genomes of the recipient strains for plasmids of the same incompatibility complex as that of P2 (Incl1). No potentially incompatible plasmids were detected in the strains based on our BLAST analysis of the conserved replication initiation protein RepZ of IncI1 plasmids (35). Interestingly, subsequent surface mating of all P3 transconjugants with a naïve recipient E. coli strain ( E. coli W3110 tsr::kmR) further confirmed that conjugative P3 transfer was only possible for donor cells inheriting both P2 and P3 plasmids (Fig S4c).…”

Section: Resultsmentioning

confidence: 99%

The mobilizable plasmid P3 of Salmonella enterica serovar Typhimurium SL1344 depends on the P2 plasmid for conjugative transfer into a broad range of bacteria in vitro and in vivo

Gaissmaier

Laganenka

Herzog

et al. 2022

Preprint

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The global rise of drug-resistant bacteria is of great concern. Conjugative transfer of antibiotic resistance plasmids contributes to this antibiotic resistance crisis. Despite the substantial progress in understanding the molecular basis of conjugation in vitro, the in vivo dynamics of intra- and interspecies conjugative plasmid transfer are much less understood. In this study, we focused on the streptomycin resistance-encoding mobilizable plasmid pRSF1010SL1344 (P3) of Salmonella enterica serovar Typhimurium (S. Tm) strain SL1344. We show that P3 is mobilized by interacting with the conjugation machinery of a second, conjugative plasmid pCol1B9SL1344 (P2) of SL1344. Thereby, P3 can be transferred into a broad range of relevant environmental and clinical bacterial isolates in vitro and in vivo. Our data suggests that S. Tm persisters in host tissues can serve as P3 reservoirs and foster transfer of both, P2 and P3 once they reseed the gut lumen. This adds to our understanding of resistance plasmid transfer in ecologically relevant niches including the mammalian gut.

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Section: Resultsmentioning

confidence: 99%

The mobilizable plasmid P3 of Salmonella enterica serovar Typhimurium SL1344 depends on the P2 plasmid for conjugative transfer into a broad range of bacteria in vitro and in vivo

Gaissmaier

Laganenka

Herzog

et al. 2022

Preprint

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“…19% of the species (nine out of 48) observed in the 56 sample-plasmid pairs were from the Pseudomonas genus, where P. fluorescens F113 and P. aeruginosa had the strong associations with pEK204. This was concerning because pEK204 has spread across pathogenic bacteria [26], and IncI1 plasmids like pEK204 promote AMR due to the presence of blaCTX-M, blaTEM, blaCMY and other ESBL genes [90][91]. We applied a novel approach of scaling the rate of pEK204-host PPIs by the level the pEK499-host PPIs, which identified numerous samples, especially ones with high numbers of pil operon genes.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…19% of these were from the Pseudomonas genus, where P. fluorescens F113 and P. aeruginosa had the strong associations with pEK204. This was concerning because pEK204 has spread across pathogenic bacteria [26], and IncI1 plasmids like pEK204 promote AMR due to the presence of blaCTX-M, blaTEM, blaCMY and other ESBL genes [92][93]. P. fluorescens F113 and P. aeruginosa were among 12 samples with high rates of PPIs with pEK204, particularly if samples that had protein interacting with pil operon proteins.…”

Section: Discussionmentioning

confidence: 99%

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Informing plasmid compatibility with bacterial hosts using protein-protein interaction data

Downing

Lee

Archbold

et al. 2022

Preprint

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The compatibility of plasmids in a new host cell is of broad significance, largely due to these plasmids' role in spreading antimicrobial resistance (AMR) and virulence factor genes. Such horizontal gene transfer can transform the host cell phenotypes, but only if the plasmid genes can be replicated, expressed and work synergistically in the new host cell. In vitro screening to evaluate this is laborious and can be informed by efficient computational analyses of plasmid-host compatibility through rates of protein-protein interactions (PPIs) between plasmid and host cell proteins. We identified instances of large excesses of such PPIs in eight plasmids of public health concern, including pOXA-48, using the PPI data of samples representative of most known bacteria (n=4,363). This identified 23 species with high rates of PPIs with four blaOXA-48-positive plasmids, such as Pseudomonas aeruginosa. This approach also identified 48 species with high compatibility with four plasmids common in Escherichia coli infections. We found a strong association of one plasmid with the fimbrial adhesin operon pil, which could enhance host cell adhesion in water environments. Our work on eight plasmids demonstrated that an excess rate of PPIs could be a sign of host-plasmid compatibility, which is important for AMR control because plasmids like pOXA-48 can jump between species with apparent ease. This compatibility could also be a sign of long-term co-evolution and therefore compensation by the host cell, implying the plasmids could be retained even in the absence of antibiotic pressure.

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“…Plasmids are circular, double-stranded DNA molecules that occur naturally in bacterial cells [1], whose genes often provide evolutionary advantages for bacteria, such as antimicrobial resistance and/or virulence [2,3]. Plasmids are important vehicles in disseminating and acquiring antibiotic resistance and virulence, and can thus constitute a major burden on human health [4]. Recent studies have suggested that the prevalence of antimicrobial resistance (AMR) is higher in Low- and Middle-income Countries (LMICs) compared to European countries and the United States [5,6].…”

Section: Introductionmentioning

confidence: 99%

Plasmid characterization in bacterial isolates of public health relevance in a tertiary healthcare facility in Kilimanjaro, Tanzania

Sengeruan

Zwetselaar²,

Kumburu³

et al. 2022

Preprint

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Plasmids are infectious double stranded DNA molecules that are found within bacteria. Horizontal gene transfer promotes successful spread of different types of plasmids within or among bacteria species, making their detection an important task for guiding clinical treatment. We used whole genome sequenced data to determine the prevalence of plasmid replicons in clinical bacterial isolates, the presence of resistance and virulence genes in plasmids, and the relationship between resistance and virulence genes within each plasmid. All bacterial sequences were de novo assembled using Unicycler before extraction of plasmids. Assembly graphs were submitted to Gplas+plasflow for plasmid prediction. The predicted plasmid components were validated using PlasmidFinder. A total of 159 (56.2%) out of 283 bacterial isolates were found to carry plasmids, with E. coli, K. pneumoniae and S. aureus being the most prevalent plasmid carriers. A total of 27 (87.1%) combined plasmids were found to carry both resistance and virulence genes compared to 4 (12.9%) single plasmids. No statistically significant correlation was found between the number of antimicrobial resistance and virulence genes in plasmids (r =-0.25, p > 0.05). Our findings show a relatively high proportion of plasmid-carrying isolates suggesting selection pressure due to antibiotic use in the hospital. Co-occurrence of antibiotic resistance and virulence genes in clinical isolates is a public health relevant problem needing attention.

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Incompatibility Group I1 (IncI1) Plasmids: Their Genetics, Biology, and Public Health Relevance

Cited by 33 publications

References 207 publications

The mobilizable plasmid P3 of Salmonella enterica serovar Typhimurium SL1344 depends on the P2 plasmid for conjugative transfer into a broad range of bacteria in vitro and in vivo

The mobilizable plasmid P3 of Salmonella enterica serovar Typhimurium SL1344 depends on the P2 plasmid for conjugative transfer into a broad range of bacteria in vitro and in vivo

Informing plasmid compatibility with bacterial hosts using protein-protein interaction data

Plasmid characterization in bacterial isolates of public health relevance in a tertiary healthcare facility in Kilimanjaro, Tanzania

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