Incompatibility and phylogenetic relationship of I-complex plasmids

Rozwandowicz, Marta; Hordijk, Joost; Bossers, Alex; Zomer, Aldert; Wagenaar, Jaap A.; Mevius, Dik; Brouwer, Michael S.M.

doi:10.1016/j.plasmid.2020.102502

Cited by 5 publications

(6 citation statements)

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“…The I-complex plasmids contain incompatibility groups I, K, B/O, L, M and Z, which share morphological and serological similarities in their pili, based on which several variants exist within the IncI group: I1 (IncIα), I1γ and I2 (IncIδ). IncI1(IncIα) and I1γ plasmids are similar [50, 51]. The PBRT kit used in the present study detects Inc Iα, Inc I1γ, I2, B/O, K, L and M within the IncI complex.…”

Section: Discussionmentioning

confidence: 99%

CRISPR-cas heterogeneity and plasmid incompatibility types in relation to virulence determinants of Shigella

Das

Doss

Mandal

2022

Journal of Medical Microbiology

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Introduction. Virulence factors (VFs) are the most potent weapon in the molecular armoury of Shigella . In bacteria, the mobile genetic elements (MGEs) are contributors to the evolution of different types of clustered regularly interspaced short palindromic repeats-CRISPR associated genes (CRISPR-cas) variants and plasmid incompatibility types. The present study explored the virulence potential of Shigella in relation to the CRISPR-cas pattern and incompatibility types among the isolates. Hypothesis/Gap Statement. The profile of the CRISPR-cas systems among clinical isolates of Shigella in India has not been reported earlier. Limited knowledge is available on the pattern of plasmid incompatibility groups among clinical isolates Shigella . The bias is always towards studying the genetic elements associated with AMR, but the present study highlights CRISPR-cas and incompatibility types among Shigella in association with virulence. Aim. We aimed to investigate the distribution of virulence factors, CRISPR-cas pattern followed by plasmid incompatibility types among Shigella isolates. Methodology. Between 2012–2017, a total of 187 isolates of Shigella were included in the study. The virulence genes' distribution was carried out. CRISPR-cas profiling followed by analysis of the repeats and spacers was carried out. PCR-based replicon typing was used to determine the incompatibility types. The interplay was statistically determined using STATA. Results. The distribution of virulence genes showed varied pattern with ipaH present in all the isolates followed by ompA (93.6 %), virF (66.8 %), ial and sen (60.4 %), set1A (39.6 %) and set1B (39 %). CRISPR 1, CRISPR 3 and Cas6-Cas5 region were dominantly conserved. Twenty-two types of spacers were identified. The CRISPR3 repeat appeared to have a highly conserved sequence. CRISPR2 being the least common CRISPR type showed a strong association with an array of virulence genes (ial-set1A-set1B-virF) while CRISPR1 being the most dominant showed the least association with virulence genes (sen-virF). The dominant plasmids were found to be belonging to the inc FII group. The incompatibility groups FII, IncIγ, U, FIIS, FIIK, K, A/C, I1alpha was found to be associated with a greater number of virulence genes. Conclusion. The isolates showed increasing diversity in their gene content that contributes to increasing heterogeneity among the isolates, which is a known virulence strategy among pathogens.

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Section: Discussionmentioning

confidence: 99%

CRISPR-cas heterogeneity and plasmid incompatibility types in relation to virulence determinants of Shigella

Das

Doss

Mandal

2022

Journal of Medical Microbiology

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“…In order to fully characterize the used strains, whole-genome sequencing using paired-end Illumina was performed, as previously described by Rozwandowicz et al (2020) . The annotation of the sequences was performed with Prokka version 1.12 ( Seemann, 2014 ) and the corresponding sequence type a was conducted with the Multi Locus Sequence Typing online tool MLST 2.0 ( Larsen et al, 2012 ), using the two available schemes ( Wirth et al, 2006 ; Jaureguy et al, 2008 ).…”

Section: Methodsmentioning

confidence: 99%

Temperature and Nutrient Limitations Decrease Transfer of Conjugative IncP-1 Plasmid pKJK5 to Wild Escherichia coli Strains

et al. 2021

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Plasmid-mediated dissemination of antibiotic resistance among fecal Enterobacteriaceae in natural ecosystems may contribute to the persistence of antibiotic resistance genes in anthropogenically impacted environments. Plasmid transfer frequencies measured under laboratory conditions might lead to overestimation of plasmid transfer potential in natural ecosystems. This study assessed differences in the conjugative transfer of an IncP-1 (pKJK5) plasmid to three natural Escherichia coli strains carrying extended-spectrum beta-lactamases, by filter mating. Matings were performed under optimal laboratory conditions (rich LB medium and 37°C) and environmentally relevant temperatures (25, 15 and 9°C) or nutrient regimes mimicking environmental conditions and limitations (synthetic wastewater and soil extract). Under optimal nutrient conditions and temperature, two recipients yielded high transfer frequencies (5 × 10–1) while the conjugation frequency of the third strain was 1000-fold lower. Decreasing mating temperatures to psychrophilic ranges led to lower transfer frequencies, albeit all three strains conjugated under all the tested temperatures. Low nutritive media caused significant decreases in transconjugants (−3 logs for synthetic wastewater; −6 logs for soil extract), where only one of the strains was able to produce detectable transconjugants. Collectively, this study highlights that despite less-than-optimal conditions, fecal organisms may transfer plasmids in the environment, but the transfer of pKJK5 between microorganisms is limited mainly by low nutrient conditions.

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“…A single motor ATPase encoding gene is predicted in T4Pb, making the extension and retraction of the pilus uncertain since two ATPases are generally present in other types of T4P (Craig et al, 2019;Ellison et al, 2019). T4Pb structures can be found encoded in all plasmid families within the I-complex (IncB/O, IncI1, IncI2, IncK, and IncZ), which were grouped based on similar morphological and serological properties of their pili (Falkow et al, 1974;Bradley, 1984;Rozwandowicz et al, 2020). The adhesin gene in I-complex plasmids is generally the last gene of the T4Pb operon and its C-terminal portion comprises a shufflon (Figure 1E).…”

Section: Mating-pair Stabilization Mechanismsmentioning

confidence: 99%

Molecular Mechanisms Influencing Bacterial Conjugation in the Intestinal Microbiota

2021

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Bacterial conjugation is a widespread and particularly efficient strategy to horizontally disseminate genes in microbial populations. With a rich and dense population of microorganisms, the intestinal microbiota is often considered a fertile environment for conjugative transfer and a major reservoir of antibiotic resistance genes. In this mini-review, we summarize recent findings suggesting that few conjugative plasmid families present in Enterobacteriaceae transfer at high rates in the gut microbiota. We discuss the importance of mating pair stabilization as well as additional factors influencing DNA transfer efficiency and conjugative host range in this environment. Finally, we examine the potential repurposing of bacterial conjugation for microbiome editing.

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Incompatibility and phylogenetic relationship of I-complex plasmids

Cited by 5 publications

References 44 publications

CRISPR-cas heterogeneity and plasmid incompatibility types in relation to virulence determinants of Shigella

CRISPR-cas heterogeneity and plasmid incompatibility types in relation to virulence determinants of Shigella

Temperature and Nutrient Limitations Decrease Transfer of Conjugative IncP-1 Plasmid pKJK5 to Wild Escherichia coli Strains

Molecular Mechanisms Influencing Bacterial Conjugation in the Intestinal Microbiota

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