Incoherent digital holographic spectral imaging with high accuracy of image pixel registration*

Ma, Fang; Wang, Xi; Bu, Yuanzhuang; Tian, Yongzhi; Du, Yanli; Gong, Qihuang; Zhuang, Ceyun; Li, Jinhai; Li, Lei

doi:10.1088/1674-1056/abd2aa

Cited by 2 publications

(1 citation statement)

References 31 publications

(27 reference statements)

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“…It provides a precise light wave control capability for digital holography and expands the application fields of optical imaging and information processing; SLM digital holography can be used to realize high-resolution, high-contrast digital holographic microscopy, for the imaging of cells, tissues, and organs, and it can be used for cell manipulation, optical capture, and the manipulation of individual biomolecules. SLM digital holography can be used to realize photorealistic three-dimensional images [17], providing a more realistic and immersive experience by generating visual 3D images in light field, large-size three-dimensional video via electronic holography using multiple spatial light modulators [18]. In biomedicine, this technique can be used to visualize anatomical structures, pathological changes, and biological processes [19].…”

Section: Introductionmentioning

confidence: 99%

Investigation of an Improved Angular Spectrum Method Based on Holography

Wu,

Yang,

Wang

et al. 2023

Photonics

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Digital holography (DH) is a novel, real-time, non-destructive, and quantitative phase-contrast imaging method that is particularly suitable for label-free live biological cell imaging and real-time dynamic monitoring. It is currently a research hotspot in the interdisciplinary field of optics and biomedical sciences, both domestically and internationally. This article proposes an improved angle spectrum algorithm based on holographic technology, which reconstructs a cellular hologram based on phase information. Optical images and chromosome cell images, reconstructed using holographic technology at different diffraction distances under the improved angle spectrum algorithm, were analyzed and compared. The optimal diffraction distance for reconstructing chromosome cell images was selected, and chromosome cell images reproduced using traditional angle spectrum algorithms, angle spectrum algorithms combined with GS, and improved angle spectrum algorithms were compared. Comparative experiments with the different models show that the proposed algorithm is superior to traditional angle spectrum algorithms in reconstructing cell images based on phase information. Furthermore, experiments have shown that images reconstructed using the improved algorithm can resolve high signal-to-noise ratio information. This algorithmic improvement provides new applications for cellular detection in clinical diagnostics and is more suitable for cell phase reconstruction in practical applications.

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Section: Introductionmentioning

confidence: 99%

Investigation of an Improved Angular Spectrum Method Based on Holography

Wu,

Yang,

Wang

et al. 2023

Photonics

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Edge enhancement in three-dimensional vortex imaging based on FINCH by Bessel-like spiral phase modulation

He,

Zhang,

et al. 2024

Opt. Express

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Edge enhancement, as an important part of image processing, has played an essential role in amplitude-contrast and phase-contrast object imaging. The edge enhancement of three-dimensional (3D) vortex imaging has been successfully implemented by Fresnel incoherent correlation holography (FINCH), but the background noise and image contrast effects are still not satisfactory. To solve these issues, the edge enhancement of FINCH by employing Bessel-like spiral phase modulation is proposed and demonstrated. Compared with the conventional spiral phase modulated FINCH, the proposed technique can achieve high-quality edge enhancement 3D vortex imaging with lower background noise, higher contrast and resolution. The significantly improved imaging quality is mainly attributed to the effective sidelobes' suppression in the generated optical vortices with the Bessel-like modulation technique. Experimental results of the small circular aperture, resolution target, and the Drosophila melanogaster verify its excellent imaging performance. Moreover, we also proposed a new method for selective edge enhancement of 3D vortex imaging by breaking the symmetry of the spiral phase in the algorithmic model of isotropic edge enhancement. The reconstructed images of the circular aperture show that the proposed method is able to enhance the edges of the given objects selectively in any desired direction.

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Incoherent digital holographic spectral imaging with high accuracy of image pixel registration*

Cited by 2 publications

References 31 publications

Investigation of an Improved Angular Spectrum Method Based on Holography

Investigation of an Improved Angular Spectrum Method Based on Holography

Edge enhancement in three-dimensional vortex imaging based on FINCH by Bessel-like spiral phase modulation

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