Inclusions or bystanders? CMV PCR sensitivity and specificity in tissue samples

Burston, J.; Hal, Sebastian Van; Dubedat, Sally; Lee, Andie

doi:10.1016/j.jcv.2017.03.008

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…International guidelines recommend IHC or tissue qPCR [ 67 , 68 ], both being the techniques currently used by various teams. The qPCR is more sensitive than IHC [ 73 , 74 , 75 , 76 ], more reliable, and independent of the observer [ 77 ]. This molecular technique may however give “false-positive” results in the case of low viral load.…”

Section: Controversial Role Of CMV Infection In Ibd Inflammatory Fmentioning

confidence: 99%

Cytomegalovirus and Inflammatory Bowel Diseases (IBD) with a Special Focus on the Link with Ulcerative Colitis (UC)

et al. 2020

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Cytomegalovirus (CMV) infects approximately 40% of adults in France and persists lifelong as a latent agent in different organs, including gut. A close relationship is observed between inflammation that favors viral expression and viral replication that exacerbates inflammation. In this context, CMV colitis may impact the prognosis of patients suffering from inflammatory bowel diseases (IBDs), and notably those with ulcerative colitis (UC). In UC, the mucosal inflammation and T helper cell (TH) 2 cytokines, together with immunomodulatory drugs used for controlling flare-ups, favor viral reactivation within the gut, which, in turn, increases mucosal inflammation, impairs corticoid and immunosuppressor efficacy (the probability of steroid resistance is multiplied by more than 20 in the case of CMV colitis), and enhances the risk for colectomy. This review emphasizes the virological tools that are recommended for exploring CMV colitis during inflammatory bowel diseases (IBD) and underlines the interest of using ganciclovir for treating flare-ups associated to CMV colitis in UC patients.

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Section: Controversial Role Of CMV Infection In Ibd Inflammatory Fmentioning

confidence: 99%

Cytomegalovirus and Inflammatory Bowel Diseases (IBD) with a Special Focus on the Link with Ulcerative Colitis (UC)

et al. 2020

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“…Clinical presentations, endoscopic features, and laboratory examinations are not specific for the diagnosis of CMV diseases of the GI tract. Histopathological examination of GI mucosal tissue is indispensable for establishing a diagnosis of CMV GI disease, and IHC staining is considered the gold standard [ 14 , 84 , 85 , 86 , 87 , 88 ]. H&E staining, viral culture, and nucleic acid testing (NAT) are also used for diagnosis [ 15 , 89 ].…”

Section: Resultsmentioning

confidence: 99%

Cytomegalovirus Diseases of the Gastrointestinal Tract in Immunocompetent Patients: A Narrative Review

Yeh,

Wu,

Chen

et al. 2024

Viruses

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Cytomegalovirus (CMV) is a potential pathogen that causes gastrointestinal (GI) tract diseases regardless of host immunity. In contrast to immunocompromised individuals, immunocompetent patients lack a comprehensive overview of the gastrointestinal manifestations. This study aims to provide a comprehensive summary of the current evidence regarding presentations, diagnostics, management, risk assessment, and outcomes in immunocompetent patients with CMV GI disease. A thorough literature search of English publications up to April 2022 was conducted across electronic databases to identify relevant articles, with eligible case series selected for detailed analysis. The majority of immunocompetent patients affected by CMV GI disease are typically elderly, critically ill, or burdened with comorbidities that compromise immunity. Clinical presentations range from subtle symptoms to severe surgical conditions, including instances of mortality. Specific clinical presentations, blood test results, or endoscopic features are lacking, necessitating reliance on histopathological tests such as immunohistochemistry staining for diagnosis. While antiviral therapy may offer benefits in improving outcomes, careful individual assessment is warranted due to diverse comorbidities and potential side effects. Mortality rates vary considerably based on underlying medical conditions and therapeutic approaches. It is imperative for clinicians to maintain vigilance for CMV GI disease among high-risk groups, despite their baseline immunocompetence, in order to enhance clinical outcomes.

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“…Current guidelines developed for clinical trial standardization consider a positive qPCR result from a GI biopsy specimen, in a compatible clinical setting, to represent possible GI disease (54). qPCR has been evaluated in both formalin᎑fixed paraffin᎑embedded and fresh tissue biopsy samples for this application; Table 3 summarizes studies reported since 2010 that evaluated tissue PCR for the diagnosis of GI CMV in transplant recipients (47,(55)(56)(57)(58). Collectively, these data indicate that qPCR on either formalin᎑fixed paraffin᎑embedded or fresh GI biopsy specimens may have an adjunctive role for the diagnosis of GI CMV disease.…”

Section: Detection Of Virus At Specific Sites Of Diseasementioning

confidence: 99%

Progress and Challenges in the Prevention, Diagnosis, and Management of Cytomegalovirus Infection in Transplantation

2020

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SUMMARY Hosts with compromised or naive immune systems, such as individuals living with HIV/AIDS, transplant recipients, and fetuses, are at the highest risk for complications from cytomegalovirus (CMV) infection. Despite substantial progress in prevention, diagnostics, and treatment, CMV continues to negatively impact both solid-organ transplant (SOT) and hematologic cell transplant (HCT) recipients. In this article, we summarize important developments in the field over the past 10 years and highlight new approaches and remaining challenges to the optimal control of CMV infection and disease in transplant settings.

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Inclusions or bystanders? CMV PCR sensitivity and specificity in tissue samples

Cited by 6 publications

References 7 publications

Cytomegalovirus and Inflammatory Bowel Diseases (IBD) with a Special Focus on the Link with Ulcerative Colitis (UC)

Cytomegalovirus and Inflammatory Bowel Diseases (IBD) with a Special Focus on the Link with Ulcerative Colitis (UC)

Cytomegalovirus Diseases of the Gastrointestinal Tract in Immunocompetent Patients: A Narrative Review

Progress and Challenges in the Prevention, Diagnosis, and Management of Cytomegalovirus Infection in Transplantation

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