Inclusion of Asparagopsis armata in lactating dairy cows’ diet reduces enteric methane emission by over 50 percent

Roque, Breanna M.; Salwen, Joan King; Kinley, Rob; Kebreab, E.

doi:10.1016/j.jclepro.2019.06.193

Cited by 223 publications

(236 citation statements)

References 35 publications

Supporting

Mentioning

215

Contrasting

Unclassified

Order By: Relevance

“…Bromoform concentration in AT was analyzed according to Roque et al [21] using an Agilent 7890B GC applied to Agilent 7000C triple quad Mass Spectrometer equipped with a ZB-5ms column (Agilent Technologies, Inc. Santa Clara, CA, USA).…”

Section: Chemical Analysismentioning

confidence: 99%

In Vitro Evaluation of Different Dietary Methane Mitigation Strategies

Chagas

Ramin

Krizsan

2019

Animals

View full text Add to dashboard Cite

Simple Summary: Dietary methane mitigation strategies do not necessarily make food production from ruminants more energy-efficient, but reducing methane (CH 4 ) in the atmosphere immediately slows down global warming, helping to keep it within 2 • C above the pre-industrial baseline. There is no single most efficient strategy for mitigating enteric CH 4 production from domestic ruminants on forage-based diets. This study assessed a wide variety of dietary CH 4 mitigation strategies in the laboratory, to provide background for future studies with live animals on the efficiency and feasibility of dietary manipulation strategies to reduce CH 4 production. Among different chemical and plant-derived inhibitors and potential CH 4 -reducing diets assessed, inclusion of the natural antimethanogenic macroalga Asparagopsis taxiformis showed the strongest, and dose-dependent, CH 4 mitigating effect, with the least impact on rumen fermentation parameters. Thus, applying Asparagopsis taxiformis at a low daily dose was the best potential dietary mitigation strategy tested, with promising long-term effects, and should be further studied in diets for lactating dairy cows. Abstract:We assessed and ranked different dietary strategies for mitigating methane (CH 4 ) emissions and other fermentation parameters, using an automated gas system in two in vitro experiments. In experiment 1, a wide range of dietary CH 4 mitigation strategies was tested. In experiment 2, the two most promising CH 4 inhibitory compounds from experiment 1 were tested in a dose-response study. In experiment 1, the chemical compounds 2-nitroethanol, nitrate, propynoic acid, p-coumaric acid, bromoform, and Asparagopsis taxiformis (AT) decreased predicted in vivo CH 4 production (1.30, 21.3, 13.9, 24.2, 2.00, and 0.20 mL/g DM, respectively) compared with the control diet (38.7 mL/g DM). The 2-nitroethanol and AT treatments had lower molar proportions of acetate and higher molar proportions of propionate and butyrate compared with the control diet. In experiment 2, predicted in vivo CH 4 production decreased curvilinearly, molar proportions of acetate decreased, and propionate and butyrate proportions increased curvilinearly with increased levels of AT and 2-nitroethanol. Thus 2-nitroethanol and AT were the most efficient strategies to reduce CH 4 emissions in vitro, and AT inclusion additionally showed a strong dose-dependent CH 4 mitigating effect, with the least impact on rumen fermentation parameters.

show abstract

Section: Chemical Analysismentioning

confidence: 99%

In Vitro Evaluation of Different Dietary Methane Mitigation Strategies

Chagas

Ramin

Krizsan

2019

Animals

View full text Add to dashboard Cite

show abstract

“…In an Australian experiment, enteric CH 4 emissions were reduced by up to 67% when the red seaweed species Asparagopsis armata was included at 1.0% of the total diet offed to dairy cows [12]. Li et al [21] investigated A. taxiformis in diets to sheep at inclusions of 0.5%, 1%, 2% and 3% and found up to an 80% reduction in enteric CH 4 compared to a control diet.…”

Section: In Vivomentioning

confidence: 99%

“…In general, the amino acid composition of Porphyra sp., is similar to proteins from soybean and eggs [9][10][11]. Palatability when feeding seaweeds to ruminants has been mentioned by, e.g., Roque et al [12] and Özkan-Gülzari et al [13] and dealt with by adding water or molasses.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro and in situ techniques are used in animal nutrition research as they are less expensive, time-consuming and laborious compared to in vivo experiments. There are some in vitro and in situ studies investigating the nutritive value of seaweeds [7][8][9]19] but, to our knowledge, very few in vivo experiments have been performed using seaweed as a protein source for ruminants [12,20,21]. Also, we have no knowledge of in vivo experiments including Porphyra sp.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ruminal Fermentation, Growth Rate and Methane Production in Sheep Fed Diets Including White Clover, Soybean Meal or Porphyra sp.

Lind

Weisbjerg

Jørgensen

et al. 2020

Animals

View full text Add to dashboard Cite

The aim of the present work was to investigate the potential of Porphyra sp. as an alternative source of protein to soybean meal in diets for sheep. Our experimental treatments included a control diet (CON) based on grass silage and crushed oats and three diets containing protein supplements, clover silage (CLO), soybean meal (SOY) or Porphyra sp. (POR) to increase dietary crude protein concentrations. We studied its effects on rumen fermentation, growth rate and methane emissions. Ruminal fermentation characteristics, kinetics of gas production and methane production were studied in vitro by using batch cultures inoculated with rumen inoculum from sheep. There were no differences among diets in total volatile fatty acids (VFA) production or in the VFA profile in vitro. Across treatments, we measured no differences in methane production either in vitro or in vivo, and we saw no noticeable antimethanogenic effect of Porphyra sp. The present in vivo trial with lambs showed no differences in average daily weight gain when fed diets including Porphyra sp. or soybean meal diets (250 and 254 g/d, respectively). We conclude that Porphyra sp. has a protein value similar to high-quality protein sources like soybean meal.

show abstract

“…Importantly, the concentration of bioactives in Asparagopsis spp, in particular bromoform, has a significant effect on the reduction of CH4 in animal trials. For example, Roque et al (2019b) tested the effects of the seaweed A. armata (1.3 mg bromoform/g DM) in vivo fed to dairy cattle for a two week duration and reported up to 67% reduction of enteric CH4 production using a 1% seaweed inclusion rate on organic matter (OM) basis in a total mixed ration (TMR). Kinley et al (2020) reported up to 98% CH4 reduction using 0.2% of OM inclusion of A. taxiformis (6.6 mg bromoform/g DM) during a 90-day feeding regime typical of feedlot TMR.…”

mentioning

confidence: 99%

Red seaweed (Asparagopsis taxiformis)supplementation reduces enteric methane by over 80 percent in beef steers

Roque

Venegas

Kinley³

et al. 2020

Preprint

View full text Add to dashboard Cite

The red macroalgae (seaweed) Asparagopsis spp. has shown to reduce ruminant enteric methane (CH4) production up to 99% in vitro. The objective of this study was to determine the effect of Asparagopsis taxiformis on CH4 production (g/day per animal), CH4 yield (g CH4/kg dry matter intake (DMI)), average daily gain (ADG), feed conversion efficiency (FCE), and carcass and meat quality in growing beef steers. Twenty-one Angus-Hereford beef steers were randomly allocated to one of three treatment groups: 0% (Control), 0.25% (Low Dose; LD), and 0.5% (High Dose; HD) A. taxiformis inclusion based on organic matter intake. Steers were fed 3 diets: high, medium, and low forage total mixed ration (TMR) representing typical life-stage diets of growing beef steers. The LD and HD treatments over 147 days reduced enteric CH4 yield 45 and 68%, respectively; however, there was an interaction between TMR type and the magnitude of CH4 yield reduction. Supplementing the low forage TMR reduced CH4 yield 69.8% (P <0.001) for LD and 80% (P <0.001) for HD treatment. Hydrogen (H2) yield (g H2/DMI) increased significantly (P<0.001) 336 and 590% compared to Control for the LD and HD treatments, respectively. No differences were found in carbon dioxide (CO2) yield (g CO2/DMI), ADG, carcass quality, strip loin proximate analysis and shear force, or consumer taste preferences. DMI tended (P = 0.08) to decrease 8% in steers in LD treatment but significantly (P = 0.002) reduced 14% in steers in HD treatment. Conversely, FCE tended to increase 7% in steers in LD treatment (P = 0.06) and increased 14% in steers in HD (P < 0.01) treatment compared to Control. The persistent reduction of CH4 by A. taxiformis supplementation suggests that this is a viable feed additive to significantly decrease the carbon footprint of ruminant livestock and potentially increase production efficiency.

show abstract

Inclusion of Asparagopsis armata in lactating dairy cows’ diet reduces enteric methane emission by over 50 percent

Cited by 223 publications

References 35 publications

In Vitro Evaluation of Different Dietary Methane Mitigation Strategies

In Vitro Evaluation of Different Dietary Methane Mitigation Strategies

Ruminal Fermentation, Growth Rate and Methane Production in Sheep Fed Diets Including White Clover, Soybean Meal or Porphyra sp.

Red seaweed (Asparagopsis taxiformis)supplementation reduces enteric methane by over 80 percent in beef steers

Contact Info

Product

Resources

About