Incisional Therapies: Trabeculectomy Surgery

Cotlear, Daniel; Melamed, Schlomo

doi:10.1007/978-1-4614-4172-4_26

Cited by 1 publication

(1 citation statement)

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“…7 However, conjunctival wound healing after such drainage surgeries can sometimes be excessive, leading to subconjunctival fibrosis and scarring at the drainage site, with progressive reduction of aqueous outflow and surgical failure. 8 Aside from surgery, long-term use of topical glaucoma medication can also activate human Tenon's fibroblasts (HTF). 9 Excessive activation of HTF result in their proliferation and migration, synthesis of extracellular matrix components (ECM), and collagen contraction, leading to subconjunctival scarring.…”

Section: Introductionmentioning

confidence: 99%

Effect of Vitamin D3 on Regulating Human Tenon's Fibroblasts Activity

Jia

Chen

Wang

et al. 2021

Trans. Vis. Sci. Tech.

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To study the in vitro effect of vitamin D3 on the healing response of human Tenon's fibroblasts (HTF) and its possible role in preventing excessive postoperative subconjunctival fibrosis.Methods: Effect of vitamin D3 on cytotoxicity and cell survival of primary cultured HTF was measured by lactate dehydrogenase and PrestoBlue assays, respectively. Proliferation and migration of vitamin D3-treated HTF (D3-HTF) was determined by CyQUANT proliferation and scratch assay, respectively. The mRNA expression profiles of control-HTF and D3-HTF from six subjects (three with glaucoma and long-term use of topical medications, three with primary pterygium) were assessed by RNA sequencing analyses to identify potential biomarkers for the inhibitory effect on HTF by vitamin D3. Validation of these biomarkers and their potential pathways were performed by quantitative real-time polymerase chain reaction (qRT-PCR) detection.Results: Pure monolayers of HTF from controls (retinal detachment or squint surgeries), pterygium, and glaucoma subjects were successfully prepared and passaged. Proliferation and migration of pterygium and glaucoma HTF were inhibited by vitamin D3 in a dose-dependent manner, and without cytotoxicity or decrease in cellular viability with concentrations up to 10 μM. The qRT-PCR results were consistent with the transcriptome analyses, vitamin D3 appears to enhance CYP24A1, SHE, KRT16 but suppresses CILP expression in HTF.Conclusions: Vitamin D3 can inhibit the in vitro activity of HTF without compromising cellular survivability at concentration up to 10 μM. This has potential clinical application for improving the outcome of pterygium and filtering surgeries.Translational Relevance: Vitamin D3 can suppress the in vitro proliferation, migration, and transdifferentiation of human Tenon's fibroblasts, without the cytotoxicity of mitomycin-C, the current standard antifibrotic agent in clinical use.

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Section: Introductionmentioning

confidence: 99%