Incidencia y agentes causales de enfermedades de raíz y sus antagonistas en manzanos de Chihuahua, México

Ruiz-Cisneros, MarÃ­a Fernanda; Rios‐Velasco, Claudio; Reyes, David; Ornelas-Paz, JosÃ© de JesÃos; Acosta-MuÃ±iz, Carlos Horacio; Romo-ChacÃ3n, Alejandro; Zamudio‐Flores, Paul Baruk; PÃ©rez-Corral, Daniel Alfonso; Salas‐Marina, Miguel Ángel; Ibarra-RendÃ3n, Eugenio; FernÃ¡ndez-PavÃ­a, Sylvia Patricia

doi:10.18781/r.mex.fit.1704-3

Cited by 6 publications

(14 citation statements)

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“…Rosales: Rosaceae) en las cuatro zonas productoras de manzana más importantes: Cuauhtémoc, Guerrero, Bachiniva y Namiquipa, Chihuahua, Méxi-co (Cuadro 1). Se recolectaron muestras de suelo (500-600 g) de la rizosfera de cinco árboles con síntomas aparentes de enfermedades radiculares causadas por hongos y oomicetos en cada huerto (Ruiz-Cisneros et al, 2017).…”

Section: Introductionunclassified

“…Rosales: Rosaceae) were sampled from June through July of 2015 in the four most important apple-producing areas: Cuauhtémoc, Guerrero, Bachiniva and Namiquipa, Chihuahua, Mexico (Table 1). Soil samples (500-600 g) were collected from the rhizosphere of five trees with apparent symptoms of root diseases caused by fungi and Oomycetes in each orchard (Ruiz-Cisneros et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…La extracción de ADN genómico (ADNg) se realizó de acuerdo con el método de Raeder y Broda (1985) y Ruiz-Cisneros et al (2017). El ADNg se utilizó para amplificar la región espaciadora interna soil and incubated at room temperature for 24 h. The leaf circles were removed, washed with sterile distilled water and dried on sterile brown paper.…”

Section: Introductionunclassified

“…Genomic DNA (gDNA) was extracted according to Raeder and Broda (1985) and Ruiz-Cisneros et al (2017). This gDNA was used to amplify the internal transcribed spacer (ITS) region of 18S rDNA gene using the universal primers ITS5 (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS4…”

Section: Introductionmentioning

confidence: 99%

“…The amplification was performed according to the methodology described by Ruiz-Cisneros et al (2017). The PCR products were examined by electrophoresis on a 1% agarose gel.…”

Section: Introductionmentioning

confidence: 99%

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Identificación morfológica y molecular de especies de Mortierella asociados a rizosfera de manzanos con síntomas de enfermedades radiculares

León

Muñoz-Castellanos

Ruiz-Cisneros

et al. 2017

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Resumen. Se aislaron especies de Mortierella enAgar Dextrosa Papa y agar V8, así como peras maduras y hojas de azalea, como sustratos trampa. Se obtuvieron 419 aislados de Mortierella, de muestras de suelo en Chihuahua, México, los cuales se clasificaron en 21 grupos de acuerdo con sus caracteres morfológicos. Mortierella se aisló con mayor Palabras clave: Mortierellales, diversidad, sustratos trampa, medio de aislamiento, patogenicidad. Morphological and molecular identification ofSe estima que solo un 5% de las especies fún-gicas existentes han sido registradas y descritas (Hawksworth, 2001). Mortierelliales es uno de los órdenes más abundantes y diversos de los hongos basales y hay cerca de 100 especies descritas en 13 géneros de la familia Mortierellaceae (Yadav et al., 2014), género al cual corresponde el hongo Mortierella. La mayoría de las especies de este género producen ácidos grasos poliinsaturados (PUFA) al convertir el exceso de azúcares y otras fuentes de carbón en lípidos bajo diferentes condiciones de fermentación (Rayaroth et al., 2016). En varios estudios se ha demostrado que algunas especies de Mortierella pueden acumular ácidos araquidónico, gamma-linolénico, eicosapentaenoico y docosahexaenoico en el micelio (Ho et al., apple rootstocks under greenhouse conditions and none was pathogenic. These species have not been reported previously in Mexico. The study showed that there are Mortierella species in the rhizosphere of apple trees in Chihuahua Mexico. These fungal isolates might produce polyunsaturated fatty acids and exert effects of elicitation in horticultural plants and fruit trees improving their resistance to multiple pathogens.Key words: Mortierellales, diversity, substrate baits, isolation medium, pathogenicity.It has been estimated that only 5% of the existing fungi species have been recorded and described (Hawksworth, 2001). The Mortierellales is one of the most abundant and diverse orders of basal fungi, with nearly 100 species described into 13 genera of the Mortierellaceae family (Yadav et al., 2014), in which the Mortierella genus is located. Most of species of this genus are able to produce polyunsaturated fatty acids (PUFAs) by converting excess of sugars and other carbon sources into lipids under different fermentation conditions (Rayaroth et al., 2016). Several studies have shown that some Mortierella species are able to accumulate arachidonic, γ-linolenic, eicosapentaenoic and docosahexaenoic acids in the mycelium (Ho et al., 2007; Dedyukhina et al., 2014). These compounds are involved in induction of resistance to phytopathogens in plants of agricultural importance (Zlotek and Wojcik, 2014). Additionally, these PUFAs are widely used as food supplements and drugs to improve the immune response in humans, giving technological importance to the Mortierella genus as an alternative source of these compounds (Dedyukhina et al., 2014). However, existing information about the diversity of Mortierellales is limited or out of date. The identification of Publicación en línea, en...

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Section: Introductionunclassified

Section: Introductionmentioning

confidence: 99%

Section: Introductionunclassified

Section: Introductionmentioning

confidence: 99%

“…The amplification was performed according to the methodology described by Ruiz-Cisneros et al (2017). The PCR products were examined by electrophoresis on a 1% agarose gel.…”

Section: Introductionmentioning

confidence: 99%

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