Incidência de vírus em videiras no Nordeste brasileiro e caracterização molecular parcial de isolados virais locais

Catarino, Aricléia de Moraes; Fajardo, T. V. M.; Pio-Ribeiro, Gilvan; Eiras, Marcelo; Nickel, Osmar

doi:10.1590/0103-8478cr20140587

Cited by 5 publications

(6 citation statements)

References 14 publications

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“…None of the evaluated samples were infected with GLRaV-4. These results confirm other results reporting high incidence of these pathogens in vineyards evaluated in other grape-growing regions of Brazil (Catarino et al, 2015;Moura et al, 2018). Probably, the infection levels found are determining detrimental effects in the yield potential and product quality (reductions in total soluble solid content and berry color intensity) of the three evaluated cultivars and vineyards as reported when healthy vines were compared to those with viral infections (Mannini & Digiaro, 2017), although this issue was not evaluated in this study.…”

Section: Virus Surveysupporting

confidence: 90%

Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil

2020

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Among grapevine (Vitis spp.) pathogens, viruses stand out, considering that this fruit plant is broadly susceptible to them and that they cause several diseases, reducing quality and yield of grape production. However, detecting and identifying viral infections in grapevines can be challenging. The objectives of this study were to detect grapevine viral pathogens by high-throughput sequencing (HTS) and identify grapevine viruses associated with plant propagation material from different origins. A comprehensive picture of a vine exhibiting virus-related symptoms was generated. The detected virome included four different pathogens and their variants. In addition, the incidence of seven viral pathogens was determined in samples collected in three vineyards of the main grape-growing region of Brazil. It is suggested that the observed infections could be related to the technological level of the production system adopted by these grape growers.

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Section: Virus Surveysupporting

confidence: 90%

Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil

2020

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“…In general, the following viruses were found in mixed infections: GCSV, family Reoviridae; GFkV and GRGV ( Ty m o v i r i d a e , genus M a c u l a v i r u s ) ; G L R a V-2 (Closteroviridae, Closterovirus); GLRaV-3, GLRaV-4 and GLRaV-4 strain 5 (Closteroviridae, Ampelovirus); GRSPaV (Betaflexiviridae, Foveavirus); Grapevine rupestris vein feathering virus (GRVFV) and GSyV-1 (Tymoviridae, Marafivirus); GVA, GVB and Grapevine virus D (GVD) (Betaflexiviridae, Vitivirus); and GVCV (Caulimoviridae, Badnavirus) (Table 1). Ten viruses had been already reported, frequently infecting grapevines in Brazil (GLRaV-2, −3, −4, −4 strain 5, GVA, GVB, GVD, GRSPaV, GFkV, GRVFV) (Basso et al 2017;Catarino et al 2015), three had not been previously reported in this country (GCSV, GRGV and GVCV) and one had only recently been identified (GSyV-1) (Moura et al 2017). It is interesting to highlight the association of some viruses with wild grapevine genotypes (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…The reaction data were analyzed as presence/absence assays and graphically, using the StepOne Software v.2.3 (Applied Biosystems), by determining the quantification cycle (Cq). Specific primers and Tamra probes used for virus detection by RT-qPCR have been previously described (Osman et al 2007;Osman and Rowhani 2008;Bianchi et al 2015;Catarino et al 2015). The presence of viruses recently detected or hitherto undetected in Brazil was confirmed by conventional RT-PCR, using the following primers: Ctg468F/Ctg468R for grapevine Cabernet Sauvignon reovirus (GCSV) (Al Rwahnih et al 2015), RG-CF-F1/RG-CF-R1 for Grapevine Red Globe virus (GRGV) (Beuve et al 2015), GSyV-1Det-F/GSyV-1Det-R for Grapevine Syrah virus 1 (GSyV-1) (Al Rwahnih et al 2009) and GVCV4142F/GVCV4387R for Grapevine vein clearing virus (GVCV) (Guo et al 2014).…”

Section: Confirmation Of Infectionsmentioning

confidence: 99%

High-throughput sequencing applied for the identification of viruses infecting grapevines in Brazil and genetic variability analysis

Fajardo

Silva

Eiras

et al. 2017

Trop. plant pathol.

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The application of high-throughput sequencing technologies (HTS) enables the recovery of many nucleotide sequence fragments from diseased plants and may help in pathogen identification. This study was designed to identify viruses infecting 15 grapevine (Vitis spp.) samples collected from experimental fields and vine collections and assess the genetic variability of the identified viruses. The virus-enriched dsRNAs were extracted from bark scrapings and sequenced using an Illumina platform. The paired-end reads were analyzed, assembled contigs were generated and identified as related to viruses. Contigs of 14 viruses have been identified, some of them covering large extensions of viral genomes or resulting in assembly of near-complete or complete genomes. Grapevine virus infections are usually mixed and the HTS assays were suitable to identify ten viruses already reported that traditionally infect grapevines in Brazil, one that has been recently identified (Grapevine Syrah virus 1) and others (Grapevine Cabernet Sauvignon reovirus, Grapevine Red Globe virus and Grapevine vein clearing virus) not previously reported in this country. Nucleotide identities among Brazilian isolates identified by HTS and homologous grapevine virus sequences in GenBank were high, ranging from 77% to 99%. Genetic variability analysis of viral sequences obtained by HTS and sequences available in GenBank indicated that the coding regions in the different viral species are under purifying selection, and that recombination events occurred in the majority of the viral species analyzed. The coat protein genes, generally, had lower genetic variability than the replicase and movement protein genes.

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“…O GLRaV-3 não foi detectado em nenhuma planta. Como as amostras indexadas provieram de vinhedos comerciais, as infecções determinadas assemelham-se àquelas obtidas em outros levantamentos (Catarino et al, 2015).…”

Section: Resultsunclassified

“…Outras práticas de manejo, que incluiriam o plantio de cultivares e porta-enxertos resistentes a vírus, não se aplicam, uma vez que não há resistência genética a vírus em cultivares comerciais de videira (Oliver et al, 2011). Em razão disso e por ser muito frequente a incidência de vírus nas principais regiões vitícolas brasileiras (Catarino et al, 2015), uma alternativa seria, em determinadas situações, a convivência com os vírus no vinhedo. A infecção viral pode resultar em maior ou menor dano, com diferentes reflexos sobre a qualidade e quantidade da produção, a depender de diversos fatores, entre os quais a gama e a intensidade dos sintomas manifestados pela planta hospedeira ou mesmo a ausência de sintomas, no caso de infecções latentes ou assintomáticas (Basso et al, 2014).…”

Section: Introductionunclassified

Desempenho agronômico de videiras com e sem sintomas de viroses, e comparação molecular de isolados virais

Nascimento

Fajardo

Eiras

et al. 2015

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar os efeitos de viroses em videiras sintomáticas e assintomáticas sobre as variáveis agronômicas relacionadas ao vigor das plantas e à qualidade enológica da uva, e comparar os isolados virais obtidos nessas duas condições. Realizaram-se dois experimentos com quatro cultivares. Todas as plantas foram indexadas, por meio da reação em cadeia da polimerase via transcrição reversa (RT-PCR) em tempo real, quanto à provável ocorrência dos seguintes vírus: Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine leafroll-associated virus (GLRaV-1 ao -4, GLRaV-4 estirpe 5), Grapevine rupestris stem pitting-associated virus (GRSPaV) e Grapevine fleck virus (GFkV). As variáveis avaliadas foram: número de gemas brotadas e não brotadas, número de ramos com ou sem cachos, número total de gemas, número de cachos, massa de cachos frescos, massa total de bagas, massa do engaço, número de bagas por cacho, massa média de baga, sólidos solúveis totais, acidez total titulável, pH, massa de ramos podados ou diâmetros do tronco do porta-enxerto e da copa. Os efeitos negativos foram mais pronunciados nas plantas com sintomas de viroses; no entanto, constatou-se frequentemente que plantas sem sintomas também estavam infectadas. A análise molecular de GRSPaV, GVA e GLRaV-2, isolados de plantas sintomáticas e assintomáticas, resultou em alta percentagem de identidade de nucleotídeos entre isolados homólogos.Termos para indexação: Grapevine, Vitis, complexo do lenho rugoso, enrolamento-da-folha, PCR em tempo real. Agronomic performance of grapevines with and without virus symptoms, and molecular comparison of viral isolatesAbstract -The objective of this work was to evaluate the effect of viroses in symptomatic and asymptomatic grapevines on the agronomic variables related to the plant vigor and the enological quality of grapes, and to compare viral isolates obtained from these two conditions. Two experiments were performed with four cultivars. All plants were indexed by real time, reverse transcription-polymerase chain reaction (RT-PCR) for the probable occurrence of the following viruses: Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine leafroll-associated virus (GLRaV-1 to -4, GLRaV-4 strain 5), Grapevine rupestris stem pitting-associated virus (GRSPaV), and Grapevine fleck virus (GFkV). The evaluated variables were: number of buds burst and not burst, number of branches with or without bunches, total number of buds, number of bunches, fresh bunch weight, total berry weight, rachis weight, number of berries per bunch, average berry weight, total soluble solids, total titratable acidity, pH, pruned branch weight, or rootstock and scion trunk diameters. The negative effects were more pronounced on the plants with virus symptoms; however, it was observed that plants without symptoms were also often infected. The molecular analysis of GRSPaV, GVA, and GLRaV-2, isolates from symptomatic and asymptomatic plants, resulted in a high perc...

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Incidência de vírus em videiras no Nordeste brasileiro e caracterização molecular parcial de isolados virais locais

Cited by 5 publications

References 14 publications

Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil

Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil

High-throughput sequencing applied for the identification of viruses infecting grapevines in Brazil and genetic variability analysis

Desempenho agronômico de videiras com e sem sintomas de viroses, e comparação molecular de isolados virais

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